Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase

Protein Engineering, Design and Selection

Volumn 21, Issue 5, 2008, Pages 319-327

  Koutsioulis, Dimitris ^a   Wang, Ellen ^b   Tzanodaskalaki, Maria ^c   Nikiforaki, Dimitra ^a   Deli, Alexandra ^a   Feller, Georges ^d   Heikinheimo, Pirkko ^b,e   Bouriotis, Vassilis ^a,c  

^a UNIVERSITY OF CRETE   (Greece)

^b UNIVERSITY OF TROMSØ   (Norway)

^c INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   (Greece)

^d UNIVERSITY OF LIÈGE   (Belgium)

^e UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Catalysis; Directed evolution; Mutagenesis; Psychrophilic enzymes; Thermostability

Indexed keywords

ENZYME ACTIVITY; ENZYMES; FOOD ADDITIVES;

ACTIVE SITES; AFTER-HEAT; ALKALINE PHOSPHATASE; CATALYSIS; DIRECTED EVOLUTION; MICHAELIS-MENTEN KINETICS; MUTAGENESIS; PSYCHROPHILIC ENZYMES; RANDOM MUTAGENESIS; RESIDUAL ACTIVITY; THERMOSTABILITY;

ACTIVATION ENERGY;

ALKALINE PHOSPHATASE;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME STABILITY; EVOLUTION; MICHAELIS MENTEN KINETICS; MICROCALORIMETRY; MOLECULAR MODEL; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; TEMPERATURE DEPENDENCE; THERMOSTABILITY; BINDING SITE; CHEMISTRY; DIFFERENTIAL SCANNING CALORIMETRY; DIMERIZATION; DIRECTED MOLECULAR EVOLUTION; GENETIC RECOMBINATION; GENETICS; KINETICS; METHODOLOGY; MUTATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; TEMPERATURE; THERMODYNAMICS;

ALKALINE PHOSPHATASE; BINDING SITES; CALORIMETRY, DIFFERENTIAL SCANNING; DIMERIZATION; DIRECTED MOLECULAR EVOLUTION; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINATION, GENETIC; TEMPERATURE; THERMODYNAMICS;

EID: 48849097115     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzn009     Document Type: Article

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