Engineering the properties of a cold active enzyme through rational redesign of the active site

European Journal of Biochemistry

Volumn 268, Issue 19, 2001, Pages 5074-5080

  Tsigos, Iason ^a   Mavromatis, Konstantinos ^b   Tzanodaskalaki, Maria ^a   Pozidis, Charalambos ^a   Kokkinidis, Michael ^a,b   Bouriotis, Vassilis ^a,b  

^a INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   (Greece)

^b UNIVERSITY OF CRETE   (Greece)

Author keywords

Alkaline phosphatase; Cold adaptation; Psychrophiles; Psychrophilic microorganisms; Structural flexibility

Indexed keywords

ALANINE; ALKALINE PHOSPHATASE; HISTIDINE; MAGNESIUM ION; TRYPTOPHAN;

ARTICLE; BINDING SITE; COLD ACCLIMATIZATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME MODIFICATION; ENZYME STABILITY; MUTAGENESIS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY;

ALKALINE PHOSPHATASE; BASE SEQUENCE; BINDING SITES; DNA PRIMERS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; TEMPERATURE;

EID: 0034787098     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02432.x     Document Type: Article

References (28)

1. Cold-adapted enzymes: From fundamentals to biotechnology
2. Toward a molecular understanding of cold activity of enzymes from psychrophiles
3. Purification and characterization of an alcohol dehydrogenase from the Antarctic psychrophile Moraxella sp. TAE123
4. Structural, kinetic, and calorimetric characterization of the cold-active phosphoglycerate kinase from the antarctic Pseudomonas sp. TACII18
5. Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties
6. Psychrophilic enzymes: Revisiting the thermodynamic parameters of activation may explain local flexibility
7. Psychrophilic enzymes: Molecular basis of cold adaptation
8. Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor
9. Structural adaptations of the cold-active citrate synthase from an Antarctic bacterium
10. Structural basis for cold adaptation. Sequence, biochemical properties, and crystal structure of malate dehydro-genase from a psychrophile Aquaspirillium arcticum
11. Hot spots in cold adaptation: Localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes
12. Directed evolution study of temperature adaptation in a psychrophilic enzyme
13. Subtilisin from psychrophilic antarctic bacteria: Characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold
14. A cold-adapted protease engineered by experimental evolution system
15. Alkaline phosphatase from the Antarctic strain TAB5. Properties and psychrophilic adaptations
16. A fine structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. 1. Purification and characterization of alkaline phosphatase
17. Gene splicing by overlap extension: Tailor-made genes using the polymerase chain reaction
18. Reaction mechanism of alkaline phosphatase based on crystal structures. Two-metal ion catalysis
19. SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling
20. Cleavage of the structural proteins during the assembly head of bacteriophage T4
21. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
22. Mutations at positions 153 and 328 in Escherichia coli alkaline phosphatase provide insight towards the structure and function of mammalian and yeast alkaline phosphatases
23. Metal-mediated protein stabilization
24. A correlation between the loss of hydrophobic core packing interactions and protein stability
25. The mechanism of the alkaline phosphatase reaction: Insights from NMR, crystallography and site-specific mutagenesis
26. Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins