Production and characterization of pharmacologically active recombinant human phosphodiesterase 4B in Dictyostelium discoideum

Biotechnology Journal

Volumn 3, Issue 7, 2008, Pages 938-947

  Arya, Ranjana ^a   Aslam, Saima ^b   Gupta, Shivani ^a   Bora, Roop Singh ^a   Vijayakrishnan, Lalitha ^a   Gulati, Pankaj ^a   Naithani, Sudha ^a   Mukherjee, Shohini ^a   Dastidar, Sunanda ^a   Bhattacharya, Alok ^b   Saini, Kulvinder Singh ^a  

^a RANBAXY RESEARCH LABORATORIES   (India)

^b JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

COPD; Dictyostelium discoideum; Expression; Phosphodiesterase; Recombinant

Indexed keywords

CELL DENSITIES; CHRONIC OBSTRUCTIVE PULMONARY DISEASE; COPD; DICTYOSTELIUM DISCOIDEUM; EXPRESSION; HIGH-THROUGHPUT ASSAYS; PHOSPHODIESTERASE; PHOSPHODIESTERASE 4B; RECOMBINANT;

ARSENIC COMPOUNDS;

CHLORINE COMPOUNDS;

9 (2 HYDROXY 3 NONYL)ADENINE; CILOMILAST; DIPYRIDAMOLE; ISOBUTYLMETHYLXANTHINE; PHOSPHODIESTERASE IV; RECOMBINANT PHOSPHODIESTERASE IVB2; RECOMBINANT PROTEIN; ROFLUMILAST; ROLIPRAM; ZAPRINAST;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL DENSITY; CELL LYSATE; CELL STRAIN HEK293; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; DRUG SYNTHESIS; ENZYME KINETICS; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IC 50; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CYCLIC NUCLEOTIDE PHOSPHODIESTERASES, TYPE 4; DICTYOSTELIUM; ENZYME INHIBITORS; GENETIC VECTORS; HUMANS; KINETICS; PLASMIDS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TRANSFORMATION, GENETIC;

DICTYOSTELIUM DISCOIDEUM; MAMMALIA;

EID: 48749128935     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200700256     Document Type: Article

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