Expression of a bioactive, single-chain choriogonadotropin in Dictyostelium discoideum

European Journal of Biochemistry

Volumn 256, Issue 2, 1998, Pages 359-363

  Heikoop, Judith C ^a,c   Grootenhuis, Peter D J ^a   Blaauw, Mieke ^b   Veldema, Jacob S ^b   Van Haastert, Peter J M ^b   Linskens, Maarten H K ^b  

^a NV ORGANON   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

^c MCKINSEY AND COMPANY   (United States)

Author keywords

Dictyostelium; Follitropin; Gonadotropin; Human choriogonadotropin; Single chain

Indexed keywords

CHORIONIC GONADOTROPIN; LUTEINIZING HORMONE RECEPTOR; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; DICTYOSTELIUM DISCOIDEUM; GENE TRANSFER; HUMAN; IMMUNOASSAY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR BINDING; RNA TRANSLATION; TECHNIQUE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHO CELLS; CHORIONIC GONADOTROPIN; CLONING, MOLECULAR; CRICETINAE; DICTYOSTELIUM; GENE EXPRESSION; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN SORTING SIGNALS; RECEPTORS, LH; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION; TRANSFORMATION, GENETIC;

ANIMALIA; DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM; MAMMALIA;

EID: 0031692251     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2560359.x     Document Type: Article

References (28)

1. Matzuk, M. M., Hsueh, A. J. W., Lapolt, P., Tsafriri, A., Keene, J. L. & Boime, I. (1990) The biological role of the carboxy-terminal extension of human chorionic gonadotropin β-subunit, Endocrinology 126, 376-383.
2. Merz, W. M. (1996) Biosynthesis of human chorionic gonadotropin: a review, Eur. J. Endocrinol. 135, 269-284.
3. Freedman, R. B. (1989) Protein disulfide isomerase. A multifunctional protein resident in the lumen of the endoplasmic reticulum, Cell 57, 581-589.
4. Feng, W., Matzuk, M. M., Mountjoy, K., Bedows, E., Ruddon, R. W. & Boime, I. (1995) The asparagine-linked oligosaccharides of the human chorionic gonadotropin beta subunit facilitate correct disulfide bond pairing. J. Biol. Chem. 270, 11851-11859.
5. Olijve, W., De Boer, W., Mulders, J. W. M. & Van Wezenbeek, P. M. G. F. (1996) Molecular biology and biochemistry of human recombinant follicle stimulating hormone (Puregon), Mol. Hum. Reprod. 2, 371-382.
6. Puett, D., Huang, J. & Xia, H. (1994) Delineation of subunit and receptor contact sites by site-directed mutagenesis of hCGβ, in Glycoprotein hormones (Lustbader, J. W., Puett, D. & Ruddon, R. W., eds) pp. 59-82, Springer Verlag-New York.
7. Fares, F. A., Suganuma, N., Nishimori, K., LaPolt, P. S., Hsueh, A. J. & Boime, I. (1992) Design of a long-acting follitropin agonist by fusing the C-terminal sequence of chorionic gonadotropin beta subunit to the follitropin beta subunit, Proc. Natl Acad. Sci. USA 89, 4304-4308.
8. Heikoop, J. C., van Beuningen-de Vaan, M. J. A. C. M., van den Boogaart, P. & Grootenhuis, P. D. J. (1997) Evaluation of subunit truncation and the nature of the spacer for single-chain gonadotropins, Eur. J. Biochem. 245, 656-662.
9. Heikoop, J. C., van den Boogaart, P., Mulders, J. & Grootenhuis, P. D. J. (1997) Structure-based design and protein engineering of intersubunit disulfide bonds in gonadotropins, Nature Biotechnol. 15, 658-662.
10. Ghetie, V., Popov, S., Borvak, J., Radu, C., Matesoi, D., Medesan, C., Ober, R. J. & Ward, S. (1997) Increasing the serum persistence of an IgG fragment by random mutagenesis, Nature Biotechnol. 15, 637-640.
11. Slade, M., Emslie, K. & Williams, K. L. (1997) Expression of recombinant glycoproteins in the simple eukaryote Dicyostelium discoideum, Biotech. Genet. Eng. Rev. 14, 1-35.
12. Jung, E. & Williams, K. L. (1997) The production of recombinant glycoproteins with special reference to simple eukaryotes including Dictyostelium discoideum, Biotechnol. Appl. Biochem. 25, 3-8.
13. Voith, G. & Dingermann, T. (1995) Expression of the human muscarinic receptor gene m2 in Dictyostelium discoideum, Biotehnology 13, 1225-1229.
14. Cohen, N. R., Knecht, D. A. & Lodish, H. F. (1996) Functional expression of rat GLUT 1 glucose transporter in Dictyostelium discoideum, Biochem. J. 315, 971-975.
15. Dingermann, T., Troidl, E.-M., Broker, M. & Nerke, K. (1991) Expression of the human antithrombin III in the cellular slime mould Dictyostelium discoideum, Appl. Microbiol. Biotechnol. 35, 496-503.
16. Kim, J.-Y., Caterina, M. J., Milne, J. L. S., Lin, K. G. Borleis, J. A. & Devreotes, P. N. (1997) Random mutagenesis of the cAMP chemoattractant receptor, cAR1, of Dictyostelium, J. Biol. Chem. 272, 2060-2068.
17. Hughes, J. E., Kiyosawa, H. & Welker, D. L. (1994) Plasmid maintenance functions encoded on Dictyostelium discoideum nuclear plasmid Dpdl, Mol. Cell. Biol. 14, 6117-6124.
18. Sutoh, K. (1993) A transformation vector for Dictyostelium discoideum with a new selectable marker bsr, Plasmid 30, 150-154.
19. Kumagai, A., Pupillo, M. Gundersen, R., Miake-Lye, R., Devreotes, P. N. & Firtel, R. A. (1989) Regulation and function of G alpha protein subunits in Dictyostelium, Cell 57, 265-275.
20. Early, H. A., Williams, J. G., Meyer, H. E., Por, S. B., Smith, E., Williams, K. L. & Gooley, A. A. (1988) Structural characterization of Dictyostelium discoideum prespore-specific gene D19 and of its product, cell surface glycoprotein PsA, Mol. Cell. Biol. 8, 3458-3466.
21. Dittrich, W., Williams, K. L. & Slade, M. B. (1994) Production and secretion of recombinant proteins in Dictyostelium discoideum, Biotechnology 12, 614-618.
22. Mann, S. K. O., Devreotes, P. N., Elliott, S., Jermyn, K., Kuspa, A., Fechheimer, M., Furukawa, R., Parent, C., Segall, J., Shaulsky, G., Vardy, P. H., Williams, J., Williams, K. L. & Firtel, R. A. (1994) Cell biological, molecular genetic, and biochemical methods to examine Dictyostelium, in Cell biology: a laboratory handbook (Celis, J. H., ed.) vol. 1, pp. 412-452, Academic Press.
23. Jia, X.-C., Oikawa, M., Bo, M., Tanaka, T., Ny, T., Boime, I. & Hsueh, A. J. W. (1991) Expression of human luteinizing hormone (LH) receptor: interaction with LH and chorionic gonadotropin from human but not equine, rat, and ovine species, Mol. Endocrinol. 5, 759-768.
24. Sharp, P. M. & Devine, M. (1989) Codon usage and gene expression level in Dictyostelium discoideum: highly expressed genes do 'prefer' optimal codons, Nucleic Acids Res. 17, 5029-5039.
25. Huth, J. R., Mountjoy, K., Perini, F. & Ruddon, R. W. (1992) Intracellular folding pathway of human chorionic gonadotropin β subunit, J. Biol. Chem. 267, 8870-8879.
26. Lapthorn, A. J., Harris, D. C., Littlejohn, A., Lustbader, J. W., Canfield, R. E., Machin, K. J., Morgan, F. J. & Isaacs, N. W. (1994) Crystal structure of human chorionic gonadotropin, Nature 369, 455-461.
27. Reddy, B. V. B., Bartozewicz, Z. & Rebois, R. V. (1996) Modification of the sialic acid residues of choriogonadotropin affects signal transduction, Cell Signal 8, 35-41.
28. Robertson, W. R., Lambert, A., Mitchell, R. & Talbot, J. A. (1997) The interpretation of immuno- and bioassays of human FSH, in FSH action and intraovarian regulation (Fauser, B. C. J. M., ed.) pp. 33-50, Parthenon, New York.