15N NMR relaxation studies of Y14F mutant of ketosteroid isomerase: The influence of mutation on backbone mobility

Journal of Biochemistry

Volumn 144, Issue 2, 2008, Pages 159-166

  Lee, Hyeong Ju ^a   Yoon, Ye Jeong ^a   Jang, Do Soo ^a,b   Kim, Chul ^a,c   Cha, Hyung Jin ^a   Hong, Bee Hak ^a   Choi, Kwan Yong ^a   Lee, Hee Cheon ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b GLADSTONE INSTITUTES   (United States)

^c California Institute of Technology   (United States)

Author keywords

Backbone dynamics; Ketosteroid isomerase; Mutant; NMR; Relaxation

Indexed keywords

19 NORTESTOSTERONE HEMISUCCINATE; MUTANT PROTEIN; STEROID; STEROID DELTA ISOMERASE; 19 NORTESTOSTERONE 17 HEMISUCCINATE; 19-NORTESTOSTERONE 17-HEMISUCCINATE; BACTERIAL PROTEIN; DRUG DERIVATIVE; NANDROLONE; NITROGEN; STEROID DELTA-ISOMERASE; TYROSINE;

ARTICLE; CATALYSIS; COMAMONAS TESTOSTERONI; CRYSTAL STRUCTURE; HETERONUCLEAR SINGLE QUANTUM COHERENCE; HYDROGEN BOND; MOLECULAR DYNAMICS; MOTION ANALYSIS SYSTEM; MUTATION; NITROGEN NUCLEAR MAGNETIC RESONANCE; NONHUMAN; NUCLEAR OVERHAUSER EFFECT; PARAMETER; PROTON NUCLEAR MAGNETIC RESONANCE; STEROID ANALYSIS; STEROID BINDING; STRUCTURE ANALYSIS; WILD TYPE; AMINO ACID SUBSTITUTION; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; NUCLEAR MAGNETIC RESONANCE;

COMAMONAS TESTOSTERONI;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BINDING SITES; COMAMONAS TESTOSTERONI; HYDROGEN BONDING; MODELS, MOLECULAR; MUTATION; NANDROLONE; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; STEROID ISOMERASES; TYROSINE;

EID: 48749103749     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvn053     Document Type: Article

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