Voltage clamp fluorimetry reveals a novel outer pore instability in a mammalian voltage-gated potassium channel

Journal of General Physiology

Volumn 132, Issue 2, 2008, Pages 209-222

  Vaid, Moninder ^a   Claydon, Thomas W ^a,b   Rezazadeh, Saman ^a   Fedida, David ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b SIMON FRASER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; FLUORESCENT DYE; SHAKER POTASSIUM CHANNEL; VOLTAGE GATED POTASSIUM CHANNEL;

ANIMAL CELL; ARTICLE; CHANNEL GATING; ELECTRIC POTENTIAL; ELECTRONIC SENSOR; FLUORESCENCE; FLUOROMETRY; GENE MUTATION; ION CURRENT; MAMMAL; MEMBRANE CONDUCTANCE; MICROPORE FILTER; MOLECULAR SENSOR; NONHUMAN; OOCYTE; POROSITY; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; VOLTAGE CLAMP; XENOPUS LAEVIS;

ANIMALS; CELL MEMBRANE; FLUOROMETRY; ION CHANNEL GATING; KV1.5 POTASSIUM CHANNEL; MAMMALS; MEMBRANE POTENTIALS; MUTATION; OOCYTES; PATCH-CLAMP TECHNIQUES; POTASSIUM; XENOPUS;

EID: 48649108570     PISSN: 00221295     EISSN: 00221295     Source Type: Journal    
DOI: 10.1085/jgp.200809978     Document Type: Article

References (59)

1. Armstrong, C.M., and A. Loboda. 2001. A model for 4-aminopyridine action on K channels: similarities to tetraethylammonium ion action. Biophys. J. 81:895-904.
2. + channel voltage sensor. Proc. Natl. Acad. Sci. USA. 102:18718-18723.
3. +]: a tale of two inactivation mechanisms. Neuron. 15:951-960.
4. + channel. Nat. Struct. Biol. 7:309-311.
5. Bruening-Wright, A., F. Elinder, and H.P. Larsson. 2007. Kinetic relationship between the voltage sensor and the activation gate in spHCN channels. J Gen. Physiol. 130:71-81.
6. Casde, N.A., S.R. Fadous, D.E. Logothetis, and G.K. Wang. 1994. 4-Aminopyridine binding and slow inactivation are mutually exclusive in rat Kv1.1 and Shaker potassium channels. Mol. Pharmacol. 46:1175-1181.
7. + channel with fluorescence. Neuron. 19:1127-1140.
8. Cha, A., and F. Bezanilla. 1998. Structural implications of fluorescence quenching in the Shaker K+ channel. J. Gen. Physiol. 112: 391-408.
9. + channel fast inactivation. Neuron. 22:73-87.
10. + channel deactivation. J Gen. Physiol. 110:87-100.
11. Claydon, T.W., M. Vaid, S. Rezazadeh, S.J. Kehl, and D. Fedida. 2007a. 4-aminopyridine prevents the conformational changes associated with P/C-type inactivation in Shaker channels. J. Pharmacol. Exp. Ther. 320:162-172.
12. + channels at low pH. J Gen. Physiol. 129:437-455.
13. Cordero-Morales, J.F., V. Jogini, A. Lewis, V. Vasquez, D.M. Cortes, B. Roux, and E. Perozo. 2007. Molecular driving forces determining potassium channel slow inactivation. Nat. Struct. Mol. Biol. 14:1062-1069.
14. + pores. Pflugers Arch. 422:354-363.
15. + channels. J. Gen. Physiol. 126:419-428.
16. Eduljee, C., T.W. Claydon, V. Viswanathan, D. Fedida, and S.J. Kehl. 2007. SCAM analysis reveals a discrete region of the pore turret that modulates slow inactivation in Kv1.5. Am. J. Physiol. Cell Physiol. 292:C1041-C1052.
17. Elinder, F., R. Mannikko, and H.P. Larsson. 2001. S4 charges move close to residues in the pore domain during activation in a K channel. J Gen. Physiol. 118:1-10.
18. Fedida, D., N.D. Maruoka, and S. Lin. 1999. Modulation of slow inactivation in human cardiac Kv1.5 channels by extra- and intracellular permeant cations. J Physiol. 515:315-329.
19. + channel. Neuron. 27:585-595.
20. + channel gating kinetics by deletions in the S3-S4 linker. Proc. Natl. Acad. Sci. USA. 98:9617-9623.
21. Hoshi, T., W.N. Zagotta, and R.W. Aldrich. 1990. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 250:533-538.
22. + channels: effects of alterations in the carboxy-terminal region. Neuron. 7:547-556.
23. Jäger, H., and S. Grissmer. 2001. Regulation of a mammalian Shaker-related potassium channel, hKv1.5, by extracellular potassium and pH. FEES Lett. 488:45-50.
24. 2+. J Physiol. 541:9-24.
25. + at the potassium channel selectivity filter. Biophys. J. 74:1840-1849.
26. Kiss, L., J. LoTurco, and S.J. Korn. 1999. Contribution of the selectivity filter to inactivation in potassium channels. Biophys. J. 76:253-263.
27. Kurata, H.T., and D. Fedida. 2006. A structural interpretation of voltage-gated potassium channel inactivation. Prog. Biophys. Mol. Biol. 92:185-208.
28. Laine, M., M.C. Lin, J.P. Bannister, W.R. Silverman, A.F. Mock, B. Roux, and D.M. Papazian. 2003. Atomic proximity between S4 segment and pore domain in Shaker potassium channels. Neuron. 39:467-481.
29. + channels. Neuron. 27:573-583.
30. Li-Smerin, Y., and KJ. Swartz. 2001. Helical structure of the COOH terminus of S3 and its contribution to the gating modifier toxin receptor in voltage-gated ion channels. J. Gen. Physiol. 117:205-218.
31. + channel. J. Gen. Physiol. 115:33-50.
32. Loboda, A., and C.M. Armstrong. 2001. Resolving the gating charge movement associated with late transitions in K channel activation. Biophys. J. 81:905-916.
33. + channel. Science. 309:897-903.
34. Long, S.B., E.B. Campbell, and R MacKinnon. 2005b. Voltage sensor of Kv1.2: structural basis of electromechanical coupling. Science. 309:903-908.
35. + channel in a lipid membranelike environment. Nature. 450:376-382.
36. + channel. J. Gen. Physiol. 112:377-389.
37. + channel's slow inactivation gate. J. Gen. Physiol. 116:623-636.
38. Lopez-Barneo, J., T. Hoshi, S.H. Heinemann, and R.W. Aldrich. 1993. Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels. Receptors Channels. 1:61-71.
39. Mannuzzu, L.M., M.M. Moronne, and E.Y. Isacoff. 1996. Direct physical measure of conformational rearrangement underlying potassium channel gating. Science. 271:213-216.
40. + channels. Neuron. 12:301-315.
41. + channels. J. Gen. Physiol. 116:181-190.
42. + channel. Proc. Natl. Acad. Sci. USA. 89:2466-2470.
43. Pathak, M., L. Kurtz, F. Tombola, and E. Isacoff. 2005. The cooperative voltage sensor motion that gates a potassium channel. J. Gen. Physiol. 125:57-69.
44. + channel. Neuron. 56:124-140.
45. + channels. Neuron. 11:353-358.
46. Piper, D.R., A. Varghese, M.C. Sanguinetti, and M. Tristani-Firouzi. 2003. Gating currents associated with intramembrane charge displacement in hERG potassium channels. Proc. Natl. Acad. Sci. USA. 100:10534-10539.
47. + channel (Kv1.4) expressed in Xenopus oocytes. J. Physiol. 489:709-721.
48. + channel, revealed by voltage-clamp fluorometry. Proc. Natl. Acad. Sci. USA. 103:12619-12624.
49. Smith, P.L., and G Yellen. 2002. Fast and slow voltage sensor movements in hERG potassium channels. J. Gen. Physiol. 119:275-293.
50. Smith-Maxwell, C.J., J.L. Ledwell, and R.W. Aldrich. 1998a. Role of the S4 in cooperativity of voltage-dependent potassium channel activation. J. Gen. Physiol. 111:399-420.
51. Smith-Maxwell, C.J., J.L. Ledwell, and R.W. Aldrich. 1998b. Uncharged S4 residues and cooperativity in voltage-dependent potassium channel activation. J. Gen. Physiol. 111:421-439.
52. + currents in the "non-conducting" Shaker potassium channel mutant W434F. J. Gen. Physiol. 112:85-93.
53. Wang, Z., and D. Fedida. 2001. Gating charge immobilization caused by the transition between inactivated states in the Kv1.5 channel. Biophys. J. 81:2614-2627.
54. + channel deactivation. J. Physiol. 515:331-339.
55. + channel Kv1.5. Biophys. J. 79:2416-2433.
56. + channel Kv1.5. J. Physiol. 523:575-591.
57. Yang, Y., Y. Yan, and F.J. Sigworth. 1997. How does the W434F mutation block current in Shaker potassium channels? J. Gen. Physiol. 109:779-789.
58. Yellen, G 1998. The moving parts of voltage-gated ion channels. Q. Rev. Biophys. 31:239-295.
59. +-free solutions at physiological pH. Cell Biochem. Biophys. 43:221-230.