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Volumn 18, Issue 16, 2008, Pages 4633-4637

A novel peptide from α5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat49-57 with cytotoxic and apoptotic effects against human cancer cells

Author keywords

Anticancer; Apoptosis; Asterina pectinifera; Cyclin B; Peptide

Indexed keywords

ARGINYLLYSYLLYSYLARGINYLARGINYLGLUTAMINYLARGINYLARGINYLARGININE; CYCLIN B; LEUCYLISOLEUCYLALANYLALANYLTYROSYLGLUTAMYLGLUTAMYLMETHIONINE; LEUCYLISOLEUCYLALANYLALANYLTYROSYLGLUTAMYLGLUTAMYLMETHIONYLGLYCYL ARGINYLLYSYLLYSYLARGINYLARGINYLGLUTAMINYLARGINYLARGINYLARGININE; LYSYLALANYLGLUTAMINYLISOLEUCYLARGINYLALANYLMETHIONYLGLUTAMYLCYSTEINYL ASPARAGINYLISOLEUCYLLEUCINE; LYSYLALANYLGLUTAMINYLISOLEUCYLARGINYLALANYLMETHIONYLGLUTAMYLCYSTEINYL ASPARAGINYLISOLEUCYLLEUCYLGLYCYLARGINYLLYSYLLYSYLARGINYLARGINYLGLUTAMINYL ARGINYLARGINYLARGININE; TYROSYLVALYLASPARAGINYLASPARTYLISOLEUCYLTYROSYLLEUCYLTYROSINE; TYROSYLVALYLASPARAGINYLASPARTYLISOLEUCYLTYROSYLLEUCYLTYROSYLGLYCYL ARGINYLLYSYLLYSYLARGINYLARGINYLGLUTAMINYLARGINYLARGINYLARGININE; UNCLASSIFIED DRUG; ANTINEOPLASTIC AGENT; PEPTIDE; PEPTIDE FRAGMENT; TAT PEPTIDE (49 57), HUMAN IMMUNODEFICIENCY VIRUS 1; TAT PEPTIDE (49-57), HUMAN IMMUNODEFICIENCY VIRUS 1; TRANSACTIVATOR PROTEIN;

EID: 48649083075     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2008.07.017     Document Type: Article
Times cited : (4)

References (40)
  • 33
    • 48649102832 scopus 로고    scopus 로고
    • note
    • 3 cells/well. Cells were incubated at 37 °C until they adhered, then the culture medium was discarded and 180 μL serum-free medium containing various concentrations of peptide was added. Twenty microliters of FBS were added to each well after the plate was incubated for 3 h. Cells were then incubated for 24, 48, and 72 h at 37 °C and 20 μL of MTT (Sigma) solution (5 mg/mL in PBS) was added. Cells were incubated at 37 °C for 4 h, and then 200 μL of DMSO (Merck) was added to each well. Plates were read on a Microplate Reader (BioRad 680) at a wavelength of 570 nm.
  • 35
    • 48649091862 scopus 로고    scopus 로고
    • note
    • 5 cells was re-suspended in 95 μL PBS, then 5 μL 0.01% acridine orange (Sigma) was added. Cells were mixed at room temperature, and then a drop of cells was enveloped with a coverslip on the slide and observed under a fluorescence microscope.
  • 38
    • 48649110538 scopus 로고    scopus 로고
    • note
    • 6 cells were re-suspended and fixed in 70% ice-cold ethanol overnight at 4 °C. After brief centrifugation, cells were washed with PBS and incubated for 30 min at 37 °C with 1 mL propidium iodide (PI, Sigma) solution (50 μg/mL PI, 50 μg/mL RNase, 10 μL/mL Triton X-100, 1 mg/mL Sodium citrate). Samples were analyzed using a FAC Scan flow cytometer (Becton Dickinson, US). ModFit software (Verity Software House, Inc.) was used to analyze the data for 20,000 cells in each test.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.