The industrialisation of cellular screening

Expert Opinion on Drug Discovery

Volumn 3, Issue 7, 2008, Pages 715-723

  Rees, Stephen ^a   Wise, Alan ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Cell based assays; Compound profiling; High throughput screening

Indexed keywords

AEQUORIN; CYTOTOXIC AGENT; HISTAMINE H1 RECEPTOR AGONIST; HISTAMINE H1 RECEPTOR ANTAGONIST;

ACCURACY; ANALYTIC METHOD; ANALYTICAL ERROR; ANIMAL CELL CULTURE; ARTICLE; ASTROCYTE; AUTOMATION; BINDING AFFINITY; BIOINFORMATICS; CELL ASSAY; CELL COUNT; CELL STRAIN HEK293; CHO CELL; CLINICAL PROTOCOL; COST CONTROL; CRYOPRESERVATION; CULTURE TECHNIQUE; CYTOLOGY; DRUG IDENTIFICATION; DRUG INDUSTRY; DRUG PROTEIN BINDING; DRUG RESEARCH; DRUG SAFETY; DRUG SCREENING; DRUG TARGETING; EMBRYONIC STEM CELL; EUKARYOTE; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL CULTURE; ILLUMINATION; INDUSTRIALIZATION; INTERMETHOD COMPARISON; MEDICAL TECHNOLOGY; MICROTITER PLATE ASSAY; MOLECULAR BIOLOGY; MONOLAYER CULTURE; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PHARMACOLOGICAL PARAMETERS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; QUALITY CONTROL; REPRODUCIBILITY; SIGNAL TRANSDUCTION; STRUCTURE ACTIVITY RELATION; TISSUE CULTURE;

EID: 48049111155     PISSN: 17460441     EISSN: None     Source Type: Journal    
DOI: 10.1517/17460441.3.7.715     Document Type: Article

References (31)

1. Archer JR. History, evolution, and trends in compound management for high throughput screening. Assay Drug Dev Technol 2004;2:675-81
2. Posner BA. High-throughput screening-driven lead discovery: meeting the challenges of finding new therapeutics. Curr Opin Drug Discov Devel 2005;8:487-94
3. Gribbon P, Andreas S. High-throughput drug discovery: what can we expect from HTS? Drug Discov Today 2005;10:17-22
4. Walters WP, Namchuck P. Designing screens: how to make your hits a hit. Nat Rev Drug Discov 2003;2:259-66
5. Pereira DA, Williams JA. Origin and evolution of high throughput screening. Br J Pharmacol 2007;152:53-61
6. Fox S, Farr-Jones S, Sopchak L, et al. High-throughput screening: update on practices and success. J Biomol Screen 2006;11:864-9
7. Moore K, Rees S. Cell-based versus isolated target screening: how lucky do you feel? J Biomol Screen 2001;6:66-74
8. Butcher EC. Can cell systems biology rescue drug discovery? Nat Rev Drug Discov 2005;4:461-7
9. Jones JO, Diamond MI. Design and implementation of cell-based assays to model human disease. ACS Chem Biol 2007;11:718-24
10. Miret JJ, Zhang JS, Min HS, et al. Multiplexed G-protein-coupled receptor Ca2+ flux assays for high-throughput screening. J Biomol Screen 2005;10:780-7
11. Burstein ES, Piu F, Ma JN, et al. Integrative functional assays, chemical genomics and high throughput screening: harnessing signal transduction pathways to a common HTS readout. Curr Pharm Des 2006;12:1717-29
12. Korn K, Krausz E. Cell-based high-content screening of small-molecule libraries. Curr Opin Chem Biol 2007;11:503-10
13. Peters MF, Knappenberger KS, Wilkins D, et al. Evaluation of cellular dielectric spectroscopy, a whole-cell, label-free technology for drug discovery on Gi-coupled. J Biomol Screen 2007;12:312-9
14. Glamann J, Hansen AJ. Dynamic detection of natural killer cell-mediated cytotoxicity and cell adhesion by electrical impedance measurements. Assay Drug Dev Technol 2006;4:555-63
15. Thomsen W, Frazer J, Unett D. Functional assays for screening GPCR targets. Curr Opin Biotechnol 2005;16:655-65
16. Lee HK, Brown SJ, Rosen H, Tobias PS. Application of beta-lactamase enzyme complementation to the high-throughput screening of toll-like receptor signaling inhibitors. Mol Pharmacol 2007;72:868-75
17. Zheng W, Spencer RH, Kiss L. High throughput assay technologies for ion channel drug discovery. Assay Drug Dev Technol 2004;2:543-52
18. Park JY, Arnaout MA, Gupta V. A simple, no-wash cell adhesion-based high-throughput assay for the discovery of small-molecule regulators of the integrin CD11b/CD18. J Biomol Screen 2007;12:406-17
19. Shi YH. Orphan nuclear receptors, excellent targets of drug discovery. Comb Chem High Throughput Screen 2006;9:683-9
20. Allen MD, DiPilato LM, Rahdar M, et al. Reading dynamic kinase activity in living cells for high-throughput screening. ACS Chem Biol 2006;1:371-6
21. Giri R, Srinivas J, Mouli KVVC. An optimal design approach for a cellular manufacturing system. Proc Ins Mech Eng J Eng Man 2007;221:1101-6
22. 5S for Operators: 5 Pillars of the Visual Workplace. Productivity Press; 1996
23. Womack JP. Lean thinking: where have we been and where are we going? Man Eng 2002;129:L2-6
24. Zhu ZR, Puglisi J, Connors D, et al. Use of cryopreserved transiently transfected cells in high-throughput pregnane X receptor transactivation assay. J Biomol Screen 2007;12:248-54
25. Cawkill D, Eaglestone SS. Evolution of cell-based reagent provision. Drug Discov Today 2007;12:820-5
26. Kunapuli P, Zheng W, Weber M, et al. Application of division arrest technology to cell-based HTS: comparison with frozen and fresh cells. Assay Drug Dev Technol 2005;3:17-26
27. Digan ME, Pou C, Niu H, Zhang JH. Evaluation of division-arrested cells for cell-based high-throughput screening and profiling. J Biomol Screen 2005;10:615-23
28. Zhang J-H, Chung DY, Oldenberg KR. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73
29. Stables J, Green A, Marshall F, et al. A bioluminescent assay for agonist activity at potentially any G-protein-coupled receptor. Anal Biochem 1997;252:115-26
30. Stables J, Mattheakis LC, Chang R, Rees S. Recombinant aequorin as reporter of changes in intracellular calcium in mammalian cells. Methods Enzymol 2000;327:456-71
31. Sullivan E, Tucker EM, Dale IL. Measurement of [Ca2+] using the Fluorometric Imaging Plate Reader (FLIPR). Methods Mol Biol 1999;114:125-33