In vivo site-specific recombination using the β-rec/six system

BioTechniques

Volumn 45, Issue 1, 2008, Pages 69-78

  Servert, Pilar ^a   Díaz, Vicente ^a   Lucas, Daniel ^a   De La Cueva, Teresa ^a   Rodriguez, Maria C ^a   García Castro, Javier ^a   Alonso, Juan C ^b   Martínez A, Carlos ^a   González, Manuel A ^a,c   Bernad, Antonio ^a,c  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^b UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^c CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES CNIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BETA SERINE RECOMBINASE; ENHANCED GREEN FLUORESCENT PROTEIN; RECOMBINASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL MATURATION; CELLULAR DISTRIBUTION; EMBRYONIC STEM CELL; FLUORESCENCE MICROSCOPY; GENE LOCUS; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MOLECULAR CLONING; MOUSE; NONHUMAN; RAGE; SPLEEN; T LYMPHOCYTE; THYMUS; TISSUE SPECIFICITY; TRANSGENIC MOUSE;

ANIMALIA; MAMMALIA; MUS MUSCULUS; PROKARYOTA;

EID: 47949115838     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112826     Document Type: Article

References (43)

1. Metzger, D. and R. Feil. 1999. Engineering the mouse genome by site-specific recombination. Curr. Opin. Biotechnol. 10:470-476.
2. Sauer, B. 1993. Manipulation of transgenes by site-specific recombination: use of Cre recombinase. Methods Enzymol. 225:890-900.
3. Sauer, B. 1994. Site-specific recombination: developments and applications. Curr. Opin. Biotechnol. 5:521-527.
4. Rice, P.A. 2005. Resolving integral questions in site-specific recombination. Nat. Struct. Mol. Biol. 12:641-643.
5. Smith, M.C. and H.M. Thorpe. 2002. Diversity in the serine recombinases. Mol. Microbiol. 44:299-307.
6. Diaz, V., F. Rojo, A.C. Martinez, J.C. Alonso, and A. Bernad. 1999. The prokaryotic beta-recombinase catalyzes site-specific recombination in mammalian cells. J. Biol. Chem. 274:6634-6640.
7. Rojo, F. and J.C. Alonso. 1995. The beta recombinase of plasmid pSM19035 binds to two adjacent sites, making different contacts at each of them. Nucleic Acids Res. 23:3181-3188.
8. Alonso, J.C., F. Weise, and F. Rojo. 1995. The Bacillussubtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the beta recombinase of plasmid pSM19035. J. Biol. Chem. 270:2938-2945.
9. Canosa, I., F. Rojo, and J.C. Alonso. 1996. Site-specific recombination by the beta protein from the streptococcal plasmid pSM19035: minimal recombination sequences and crossing over site. Nucleic Acids Res. 24:2712-2717.
10. Rojo, F., F. Weise, and J.C. Alonso. 1993. Purification of the beta product encoded by the streptococcus pyogenes plasmid pSM19035. A putative DNA recombinase required to resolve plasmid oligomers. FEBS Lett. 328:169-173.
11. Sorrell, D.A. and A.F. Kolb. 2005. Targeted modification of mammalian genomes. Biotechnol. Adv. 23:431-469.
12. Gu, H., J.D. Marth, P.C. Orban, H. Mossmann, and K. Rajewsky. 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265:103-106.
13. Ioffe, E. and P. Stanley. 1994. Mice lacking N- acetylglucosaminyltransferase I activity die at mid-gestation, revealing an essential role for complex or hybrid N-linked carbohydrates. Proc. Natl. Acad. Sci. USA 91:728-732.
14. Rossant, J. and A. McMahon. 1999. "Cre"-ating mouse mutants - a meeting review on conditional mouse genetics. Genes Dev. 13:142-145.
15. Agah, R., P.A. Frenkel, B.A. French, L.H. Michael, P.A. Overbeek, and M.D. Schneider. 1997. Gene recombination in postmitotic cells. Targeted expression of Cre recombinase provokes cardiac-restricted, site-specific rearrangement in adult ventricular muscle in vivo. J. Clin. Invest. 100:169-179.
16. Barlow, C., M. Schroeder, J. Lekstrom-Himes, H. Kylefjord, C.X. Deng, A. Wynshaw-Boris, B.M. Spiegelman, and K.G. Xanthopoulos. 1997. Targeted expression of Cre recombinase to adipose tissue of transgenic mice directs adipose-specific excision of loxP-flanked gene segments. Nucleic Acids Res. 25:2543-2545.
17. Chang, B.H., W. Liao, L. Li, M. Nakamuta, D. Mack, and L. Chan. 1999. Liver-specific inactivation of the abetalipoproteinemia gene completely abrogates very low density lipoprotein/low density lipoprotein production in a viable conditional knockout mouse. J. Biol. Chem. 274:6051-6055.
18. Utomo, A.R., A.Y. Nikitin, and W.H. Lee. 1999. Temporal, spatial, and cell type-specific control of Cre-mediated DNA recombination in transgenic mice. Nat. Biotechnol. 17:1091-1096.
19. Zubko, E., C. Scutt, and P. Meyer. 2000. Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat. Biotechnol. 18:442-445.
20. Awatramani, R., P. Soriano, C. Rodriguez, J.J. Mai, and S.M. Dymecki. 2003. Cryptic boundaries in roof plate and choroid plexus identified by intersectional gene activation. Nat. Genet. 35:70-75.
21. Chen, L. and S.L. Woo. 2005. Complete and persistent phenotypic correction of phenylketonuria in mice by site-specific genome integration of murine phenylalanine hydroxylase cDNA. Proc. Natl. Acad. Sci. USA 102:15581-15586.
22. Ginsburg, D.S. and M.P. Calos. 2005. Site-specific integration with phiC31 integrase for prolonged expression of therapeutic genes. Adv. Genet. 54:179-187.
23. Lewandoski, M. 2001. Conditional control of gene expression in the mouse. Nat. Rev. Genet. 2:743-755.
24. Servert, P., J. Garcia-Castro, V. Diaz, D. Lucas, M.A. Gonzalez, A.C. Martinez, and A. Bernad. 2006. Inducible model for beta-six-mediated site-specific recombination in mammalian cells. Nucleic Acids Res. 34:e1.
25. Meyers, E.N., M. Lewandoski, and G.R. Martin. 1998. An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat. Genet. 18:136-141.
26. Murray, S.A., E.A. Carver, and T. Gridley. 2006. Generation of a Snail1 (Snai1) conditional null allele. Genesis 44:7-11.
27. Diaz, V., P. Servert, I. Prieto, M.A. Gonzalez, A.C. Martinez, J.C. Alonso, and A. Bernad. 2001. New insights into host factor requirements for prokaryotic beta-recombinase-mediated reactions in mammalian cells. J. Biol. Chem. 276:16257-16264.
28. Gagneten, S., Y. Le, J. Miller, and B. Sauer. 1997. Brief expression of a GFPcre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Nucleic Acids Res. 25:3326-3331.
29. Orban, P.C., D. Chui, and J.D. Marth. 1992. Tissue- and site-specific DNA recombination in transgenic mice. Proc. Natl. Acad. Sc. USA 89:6861-6865.
30. Bouvier, G., F. Watrin, M. Naspetti, C. Verthuy, P. Naquet, and P. Ferrier. 1996. Deletion of the mouse T-cell receptor beta gene enhancer blocks alphabeta T-cell development. Proc. Natl. Acad. Sci. USA 93:7877-7881.
31. Rojo, F. and J.C. Alonso. 1994. A novel site-specific recombinase encoded by the Streptococcus pyogenes plasmid pSM19035. J. Mol. Biol. 238:159-172.
32. Alonso, J.C., C. Gutierrez, and F. Rojo. 1995. The role of chromatin-associated protein Hbsu in beta-mediated DNA recombination is to facilitate the joining of distant recombination sites. Mol. Microbiol. 18:471-478.
33. Wildin, R.S., A.M. Garvin, S. Pawar, D.B. Lewis, K.M. Abraham, K.A. Forbush, S.F. Ziegler, J.M. Allen, and R.M. Perlmutter. 1991. Developmental regulation of lck gene expression in T lymphocytes. J. Exp. Med. 173:383-393.
34. Schorle, H., T. Holtschke, T. Hunig, A. Schimpl, and I. Horak. 1991. Development and function of T cells in mice rendered interleukin-2 deficient by gene targeting. Nature 352:621-624.
35. Kuhn, R., K. Rajewsky, and W. Muller. 1991. Generation and analysis of interleukin-4 deficient mice. Science 254:707-710.
36. Sauer, B. and N. Henderson. 1988. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc. Natl. Acad. Sci. USA 85:5166-5170.
37. Dymecki, S.M. 1996. Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice. Proc. Natl. Acad. Sci. USA 93:6191-6196.
38. Thyagarajan, B., M.J. Guimaraes, A.C. Groth, and M.P. Calos. 2000. Mammalian genomes contain active recombinase recognition sites. Gene 244:47-54.
39. Schmidt, E.E., D.S. Taylor, J.R. Prigge, S. Barnett, and M.R. Capecchi. 2000. Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids. Proc. Natl. Acad. Sci. USA 97:13702-13707.
40. Loonstra, A., M. Vooijs, H.B. Beverloo, B.A. Allak, E. van Drunen, R. Kanaar, A. Berns, and J. Jonkers. 2001. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc. Natl. Acad. Sci. USA 98:9209-9214.
41. McGuire, E.A., C.E. Rintoul, G.M. Sclar, and S.J. Korsmeyer. 1992. Thymic overexpression of Ttg-1 in transgenic mice results in T-cell acute lymphoblastic leukemia/lymphoma. Mol. Cell. Biol. 12:4186-4196.
42. Omri, B., C. Blancher, B. Neron, M.C. Marty, J. Rutin, T.J. Molina, B. Pessac, and P. Crisanti. 1998. Retinal dysplasia in mice lacking p56lck. Oncogene 16:2351-2356.
43. Gronlund, J.T., C. Stemmer, J. Lichota, T. Merkle, and K.D. Crasser. 2007. Functionality of the beta/six site-specific recombination system in tobacco and Arabidopsis: a novel tool for genetic engineering of plant genomes. Plant Mol. Biol. 63:545-556.