Subcellular localization and functional expression of the glycerol uptake protein 1 (GUP1) of Saccharomyces cerevisiae tagged with green fluorescent protein

Biochemical Journal

Volumn 390, Issue 1, 2005, Pages 145-155

  Bleve, Gianluca ^a   Zacheo, Giuseppe ^a   Cappello, Maria Stella ^a   Dellaglio, Franco ^b   Grieco, Francesco ^a  

^a INSTITUTE OF SCIENCES OF FOOD PRODUCTION   (Italy)

^b UNIVERSITY OF VERONA   (Italy)

Author keywords

Endocytosis; Green fluorescent protein (GFP); GUP1; Localization; Protein topology; Yeast

Indexed keywords

FUNGI; GLYCEROL; YEAST;

ENDOCYTOSIS; GREEN FLUORESCENT PROTEINS (GFP); GUP 1; LOCALIZATION; PROTEIN TOPOLOGY;

PROTEINS;

CARBON; GLUCOSE; GLYCEROL; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN; PROTEIN GLYCEROL UPTAKE 1; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELLULAR DISTRIBUTION; CONFOCAL LASER MICROSCOPY; CULTURE MEDIUM; DOWN REGULATION; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; IMMUNOELECTRON MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; YEAST CELL;

CARRIER PROTEINS; CELL MEMBRANE; ENDOPLASMIC RETICULUM; GENE EXPRESSION REGULATION, FUNGAL; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MUTATION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

AEQUOREA VICTORIA; CHIMAERIFORMES; SACCHAROMYCES CEREVISIAE;

EID: 23944501318     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20042045     Document Type: Article

References (51)

1. Hohmann, S. and Mager, W. H. (1997) Yeast Stress Responses, R.G. Landes Co., Austin, TX
2. Hohmann, S. (2002) Osmotic stress signaling and osmoadaptation in yeasts. Microbiol. Mol. Biol. 66, 300-372
3. Blomberg, A. and Adler, L. (1992) Physiology of osmotolerance in fungi. Adv. Microb. Physiol. 33, 145-212
4. Somero, G. N. and Yancey, P. H. (1997) Osmolytes and cell volume regulation: physiological and evolutionary principles. In Handbook of Physiology, Section 14: Cell Physiology (Hoffmann, J. F. and Jamieson J. D., eds.), pp. 441-484, Oxford University Press, New York
5. Lages, F. and Lucas, C. (1997) Contribution to the physiological characterization of glycerol active uptake in Saccharomyces cerevisiae. Biochim. Biophys. Acta 1322, 8-18
6. Blomberg, A. (1997) Osmoresponsive proteins and functional assessment strategies in Saccharomyces cerevisiae. Electrophoresis 18, 1429-1440
7. Holst, B., Lunde, C., Lages, F., Oliveira, R., Lucas, C. and Kielland-Brandt, M. C. (2000) GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae. Mol. Microbiol. 37, 108-124
8. Oelkers, P., Tinkelenberg, A., Erdeniz, N., Cromley, D., Billheimer, J. T. and Sturley, S. L. (2000) A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast. J. Biol. Chem. 275, 15609-15612
9. Bonangelino, C. J., Chavez, E. M. and Bonifacino, J. S. (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol. Biol. Cell 13, 2486-2501
10. Ni, L. and Snyder, M. (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol. Biol. Cell 12, 2147-2170
11. Nelissen, B., De Wachter, R. and Goffeau, A. (1997) Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of Saccharomyces cerevisiae. FEMS Microbiol. Rev. 21, 113-134
12. Hofmann, K. (2000) A superfamily of membrane-bound O-acyltransferases with implications for Wnt signaling. Trends Biochem. Sci. 25, 111-112
13. De Hertogh, B., Carvajal, E., Talla, E., Dujon, B., Baret, P. and Goffeau, A. (2002) Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae. Funct. Integr. Genomics 2, 154-170
14. Neves, L., Lages, F. and Lucas, C. (2004) New insights on glycerol transport in Saccharomyces cerevisiae. FEBS Lett. 565, 160-162
15. Tsien, R. Y. (1998) The green fluorescent protein. Annu. Rev. Biochem. 67,509-544
16. Tran, P. T., Paoletti, A. and Chang, F. (2004) Imaging green fluorescent protein fusions in living fission yeast cells. Methods 33, 220-225
17. Ausubel, F., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (1999) Short Protocols in Molecular Biology, 4th edn, John Wiley, New York
18. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press, Plainview, NY
19. Cormack, B. P., Bertram, G., Egerton, M., Gow, N. A., Falkow, S. and Brown, A. J. (1997) Yeast-enhanced green fluorescent protein (yEGFP) a reporter of gene expression in Candida albicans. Microbiology 143, 303-311
20. Gietz, R. D., Schiestl, R. H., Willems, A. R. and Woods, R. A. (1995) Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11, 355-360
21. Longtine, M. S., McKenzie, III, A., Demarini, D. J., Shah, N. G., Wach, A., Brachat, A., Philippserif P. and Pringle, J. R. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14, 953-961
22. Amberg, D. C., Botstein, D. and Beasley, E. M. (1995) Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction. Yeast 11, 1275-1280
23. Wach, A. (1996) PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12, 259-265
24. Paiva, S., Kruckeberg, A. L. and Casal, M. (2002) Utilization of green fluorescent protein as a marker for studying the expression and turnover of the monocarboxylate permease Jen1p of Saccharomyces cerevisiae. Biochem. J. 363, 737-744
25. Grieco, F., Castellano, M. A., Di Sansebastiano, G. P., Maggipinto, G., Neuhaus, J. M. and Martelli, G. P. (1999) Subcellular localization and in vivo identification of the putative movement protein of olive latent virus 2. J. Gen. Virol. 80, 1103-1109
26. Hay, J., Grieco, F., Druka, A., Pinner, M., Lee, S. C. and Hull, R. (1994) Detection of rice tungro bacilliform virus gene products in vivo. Virology 205, 430-437
27. Garnier, C., Blondel, M. O. and Haguenauer-Tsapis, R. (1996) Membrane topology of the yeast uracil permease. Mol. Microbiol. 21, 1061-1073
28. Novick, P., Field, C. and Schekman, R. (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell (Cambridge, Mass.) 21, 205-215
29. Tschopp, J., Esmon, P. C. and Schekman, R. (1984) Defective plasma membrane assembly in yeast secretory mutants. J. Bacteriol. 160, 966-970
30. Raths, S., Rohrer, J., Crausaz, F. and Riezman, H. (1993) end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J. Cell Biol. 120, 55-65
31. Kruckeberg, A. L., Ye, L., Berden, J. A. and van Dam, K. (1999) Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein. Biochem. J. 339, 299-307
32. Diderich, J. A., Schuurmans, J. M., Van Gaalen, M. C., Kruckeberg, A. L. and Van Dam, K. (2001) Functional analysis of the hexose transporter homologue HXT5 in Saccharomyces cerevisiae. Yeast 18, 1515-1524
33. Ye, L., Berden, J. A., van Dam, K. and Kruckeberg, A. L. (2001) Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae. Yeast 18, 1257-1267
34. Petersson, J., Pattison, J., Kruckeberg, A. L., Berden, J. A. and Persson, B. L. (1999) Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae. FEBS Lett. 462, 37-42
35. Malinska, K., Malinsky, J., Opekarova, M. and Tanner, W. (2003) Visualization of protein compartmentation within the plasma membrane of living yeast cells. Mol. Biol. Cell 14, 4427-4436
36. Hood, J. K., Hwang, W. W. and Silver, P. A. (2001) The Saccharomyces cerevisiae cyclin Clb2p is targeted to multiple subcellular locations by cis- and trans-acting determinants. J. Cell Sci. 114, 589-597
37. Dupre, S. and Haguenauer-Tsapis, R. (2001) Deubiquitination step in the endocytic pathway of yeast plasma membrane proteins: crucial role of Doa4p ubiquitin isopeptidase. Mol. Cell. Biol. 21, 4482-4494
38. Holzer, H. (1976) Catabolite inactivation in yeast. Trends Biochem. Sci. 1,178-181
39. Chvatchko, Y., Howald, I. and Riezman, H. (1986) Two yeast mutants defective in endocytosis are defective in pheromone response. Cell (Cambridge, Mass.) 46, 355-364
40. Horak, J. (2003) The role of ubiquitin in down-regulation and intracellular sorting of membrane proteins: insights from yeast. Biochim. Biophys. Acta 1614, 139-155
41. Holcomb, C. L., Hansen, W. J., Etcheverry, T. and Schekman, R. (1988) Secretory vesicles externalize the major plasma membrane ATPase in yeast. J. Cell Biol. 106, 641-648
42. Azuma, M., Levinson, J. N., Page, N. and Bussey, H. (2002) Saccharomyces cerevisiae Big1p, a putative endoplasmic reticulum membrane protein required for normal levels of cell wall beta-1,6-glucan. Yeast 19, 783-793
43. Rapp, M., Drew, D., Daley, D. O., Nilsson, J., Carvalho, T., Melén, K., De Gier, J.-W. and Von Heijne, G. (2004) Experimentally based topology models for E. coli inner membrane proteins. Protein Sci. 13, 937-945
44. Ki, J. J., Kawarasaki, Y., Gam, J., Harvey, B. R., Iverson, B. L. and Georgiou, G. (2004) A periplasmic fluorescent reporter protein and its application in high-throughput membrane protein topology analysis. J. Mol. Biol. 341, 901-909
45. van der Leij, F. R., Kram, A. M., Bartelds, B., Roelofsen, H., Smid, G. B., Takens, J., Zammit, V. A. and Kuipers, J. R. (1999) Cytological evidence that the C-terminus of carnitine palmitoyltransferase I is on the cytosolic face of the mitochondrial outer membrane. Biochem. J. 341, 777-784
46. Marini, A. M. and Andre, B. (2000) In vivo N-glycosylation of the mep2 high-affinity ammonium transporter of Saccharomyces cerevisiae reveals an extracytosolic N-terminus. Mol. Microbiol. 38, 552-564
47. Klomp, A. E., Juijn, J. A., van der Gun, L. T., van den Berg, I. E., Berger, R. and Klomp, L. W. (2003) The N-terminus of the human copper transporter 1 (hCTR1) is localized extracellularly, and interacts with itself. Biochem. J. 370, 881-889
48. Cartwright, C. P. and Tipper, D. J. (1991) In vivo topological analysis of Ste2, a yeast plasma membrane protein, by using beta-lactamase gene fusions. Mol. Cell. Biol. 11, 2620-2628
49. 2 terminus. J. Biol. Chem. 272, 23623-23630
50. Thomas, B. J. and Rothstein, R. (1989) Elevated recombination rates in transcriptionally active DNA. Cell (Cambridge, Mass.) 56, 619-630
51. Nakamoto, R. K., Rao, R. and Slayman, C. W. (1991) Expression of the yeast plasma membrane [H+]ATPase in secretory vescicles. A new strategy for directed mutagenesis. J. Biol. Chem. 266, 7940-7949