Structural and functional characterization of "laboratory evolved" cytochrome P450cam mutants showing enhanced naphthalene oxygenation activity

Biochemical and Biophysical Research Communications

Volumn 323, Issue 4, 2004, Pages 1209-1215

  Matsuura, Koji ^a   Tosha, Takehiko ^a   Yoshioka, Shiro ^a   Takahashi, Satoshi ^a   Ishimori, Koichiro ^a   Morishima, Isao ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

C H bond activation; Cytochrome P450; Dioxygen; Electronic absorption; Gas chromatographic analysis; Hydrogen peroxide; Laboratory evolution; Naphthalene oxidation; P420 form; Resonance Raman

Indexed keywords

CAMPHOR 5 MONOOXYGENASE; HYDROGEN PEROXIDE; NAPHTHALENE;

ABSORPTION; ARTICLE; BINDING SITE; CATALYSIS; CATALYST; CELL ASSAY; CHEMICAL REACTION; ENZYME STABILITY; FLUORESCENCE; GAS CHROMATOGRAPHY; HYDROXYLATION; MUTANT; OXYGENATION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RAMAN SPECTROMETRY; STRUCTURE ANALYSIS; WILD TYPE;

AMINO ACID SUBSTITUTION; CAMPHOR 5-MONOOXYGENASE; ENZYME ACTIVATION; ENZYME STABILITY; MUTAGENESIS, SITE-DIRECTED; MUTATION; NAPHTHALENES; OXIDATION-REDUCTION; OXYGEN; PROTEIN CONFORMATION; QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP; RECOMBINANT PROTEINS;

EID: 4644299673     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.08.221     Document Type: Article

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