Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions

Journal of Molecular Microbiology and Biotechnology

Volumn 7, Issue 4, 2004, Pages 182-196

  Inui, Masayuki ^a   Murakami, Shikiko ^a   Okino, Shohei ^a   Kawaguchi, Hideo ^a   Vertès, Alain A ^a   Yukawa, Hideaki ^a  

^a RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH   (Japan)

Author keywords

Corynebacterium glutamicum; Lactate production; NAD+ NADH ratio; Oxygen deprivation; Succinate production

Indexed keywords

GENE PRODUCT; LACTIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; PYRUVATE CARBOXYLASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; SUCCINIC ACID;

ARTICLE; BACTERIAL STRAIN; CORYNEBACTERIUM GLUTAMICUM; GENE DISRUPTION; GENE REPLACEMENT THERAPY; GENETIC CODE; GENETIC ENGINEERING; GLUCOSE INTAKE; HYPOXIA; METABOLIC ACTIVATION; METABOLIC ENGINEERING; NONHUMAN;

ACIDS; ANAEROBIOSIS; CORYNEBACTERIUM; DNA, BACTERIAL; GENE DELETION; GENE DOSAGE; GENES, BACTERIAL; GLUCOSE; L-LACTATE DEHYDROGENASE; LACTIC ACID; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; MUTATION; NAD; PHOSPHOENOLPYRUVATE CARBOXYLASE; PYRUVIC ACID; SEQUENCE ANALYSIS, DNA; SODIUM BICARBONATE; SUCCINIC ACID;

CORYNEBACTERIACEAE; CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM;

EID: 4644247295     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000079827     Document Type: Article

References (59)

1. Billman-Jacobe, H., Wang, L., Kortt, A., Stewart, D., and Radford, A. 1995. Expression and secretion of heterologous proteases by Corynebacterium glutamicum. Appl. Environ. Microbiol. 61:1610-1613.
2. Bunch, P.K., Mat-Jan, F., Lee, N., and Clark, D.P. 1997. The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli. Microbiology 143:187-195.
3. Clark, D.P. 1989. The fermentation pathways of Escherichia coli. FEMS Microbiol. Rev. 5:223-234.
4. Danshina, P.V., Schmalhausen, E.V., Avetisyan, A.V., and Muronetz, V.I. 2001. Mildly oxidized glyceraldehyde-3-phosphate dehydrogenase as a possible regulator of glycolysis. IUBMB Life 51:309-314.
5. Dominguez, H., Nezondet, C., Lindley, N.D., and Cocaign, M. 1993. Modified carbon flux during oxygen limited growth of Corynebacterium glutamicum and the consequences for amino acid overproduction. Biotechnol. Lett. 15:449-454.
6. Donnelly, M.I., Millard, C.S., Clark, D.P., Chen, M.J., and Rathke, J.W. 1998. A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol. Appl. Biochem. Biotechnol. 70-72:187-198.
7. Duggleby, R.G., and Dennis, D.T. 1974. Nicotinamide adenine dinucleotide-specific glyceraldehyde 3-phosphate dehydrogenase from Pisum sativum. Assay and steady state kinetics. J. Biol. Chem. 249:167-174.
8. Feir, H.A., and Suzuki, I. 1969. Pyruvate carboxylase of Aspergillus niger: Kinetic study of a biotin-containing carboxylase. Can. J. Biochem. 47:697-710.
9. Glassner, D.A., and Datta, R. 1992. Process for the production and purification of succinic acid. USA patent 5143834.
10. Gokarn, R.R., Eiteman, M.A., and Altman, E. 2000. Metabolic analysis of Escherichia coli in the presence and absence of the carboxylating enzymes phosphoenolpyruvate carboxylase and pyruvate carboxylase. Appl. Environ. Microbiol. 66:1844-1850.
11. Gottschalk, G. 1985. Bacterial Metabolism. New York, Springer.
12. Gubler, M., Park, S.M., Jetten, M.S.M., Stephanopoulos, G., and Sinskey, A.J. 1994. Effects of phosphoenolpyruvate carboxylase deficiency on metabolism and lysine production in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 40:857-863.
13. Gurr, J.A., and Jones, K.M. 1977. Purification and characterization of pyruvate carboxylase from Arthrobacter globiformis. Arch. Biochem. Biophys. 179:444-455.
14. Hill, R.L., and Bradshaw, R.A. 1969. Fumarase. Methods Enzymol. 8:91-99.
15. Inui, M., Dumay, V., Zahn, K., Yamagata, H., and Yukawa, H. 1997. Structural and functional analysis of the phosphoenolpyruvate carboxylase gene from the purple nonsulfur bacterium Rhodopseudomonas palustris No. 7. J. Bacteriol. 179:4942-4945.
16. Inui, M., Nakata, K., Roh, J.H., Zahn, K., and Yukawa, H. 1999. Molecular and functional characterization of the Rhodopseudomonas palustris No. 7 phosphoenolpyruvate carboxykinase gene. J. Bacteriol. 181:2689-2696.
17. Jain, M.K., Datta, R., and Zeikus, J.G. 1989. Highvalue organic acids fermentation-emerging processes and product; in Ghose, T.K. (ed.): Bioprocess Engineering: The First Generation. Chichester, Ellis Horwood, pp. 366-389.
18. Jetten, M.S.M., and Sinskey, A.J. 1993. Characterization of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum. FEMS Microbiol. Lett. 111:183-188.
19. Jetten, M.S.M., and Sinskey, A.J. 1995. Purification and properties of oxaloacetate decarboxylase from Corynebacterium glutamicum. Antonie Van Leeuwenhoek 67:221-227.
20. Kinoshita, S., Udaka, S., and Shimono, M. 1957. Studies on the amino acid fermentation. I. Production of L-glutamic acid by various microorganisms. J. Gen. Appl. Microbiol. 3:193-205.
21. Klingenberg, M. 1989. Nicotinamide-adenine dinucleotides and dinucleotide phosphates (NAD, NADP, NADH, NADPH); in Bergmeyer, H.U., Bergmeyer, J., and Grassl, M. (eds.): Methods of Enzymatic Analysis. Weinheim, VCH Verlagsgesellschaft, vol. VII, pp. 251-271.
22. Kotrba, P., Inui, M., and Yukawa, H. 2001. The ptsI gene encoding enzyme I of the phosphotransferase system of Corynebacterium glutamicum. Biochem. Biophys. Res. Commun. 289:1307-1313.
23. Laivenieks, M., Vieille, C., and Zeikus, J.G. 1997. Cloning, sequencing, and overexpression of the Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase (pckA) gene. Appl. Environ. Microbiol. 63:2273-2280.
24. Lee, P.C., Lee, W.G., Kwon, S., Lee, S.Y., and Chang, H.N. 1999. Succinic acid production by Anaerobiospirillum succiniciproducens: Effects of the H2/CO2 supply and glucose concentration. Enzyme Microb. Technol. 24:549-554.
25. Lee, P.C., Lee, W.G., Kwon, S., Lee, S.Y., and Chang, H.N. 2000. Batch and continuous cultivation of Anaerobiospirillum succiniciproducens for the production of succinic acid from whey. Appl. Microbiol. Biotechnol. 54:23-27.
26. Liebl, W. 1992. The genus Corynebacterium - nonmedical; in Balows, A., Truper, H.G., Dworkin, M., Harder, W., and Schleifer, K.H. (eds.): The Prokaryotes. New York, Springer, vol. II, pp. 1157-1171.
27. Millard, C.S., Chao, Y.P., Liao, J.C., and Donnelly, M.I. 1996. Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli. Appl. Environ. Microbiol. 62:1808-1810.
28. Molenaar, D., van der Rest, M.E., Drysch, A., and Yucel, R. 2000. Functions of the membraneassociated and cytoplasmic malate dehydrogenases in the citric acid cycle of Corynebacterium glutamicum. J. Bacteriol. 182:6884-6891.
29. Nakata, K., Inui, M., Kos, P.B., Vertes, A.A., and Yukawa, H. 2003. Vectors for the genetics engineering of corynebacteria; in Saha, B.C. (ed.): Fermentation Biotechnology, ACS Symposium Series 862. Washington, American Chemical Society, pp. 175-191.
30. Nakayama, K., Kitada, S., and Kinoshita, S. 1961. Studies on lysine fermentation I. The control mechanism on lysine accumulation by homoserine and threonine. J. Gen. Appl. Microbiol. 7:145-154.
31. Park, S.M., Shaw-Reid, C., Sinskey, A.J., and Stephanopoulos, G. 1997. Elucidation of anaplerotic pathway in Corynebacterium glutamicum via 13C-NMR spectroscopy and GC-MS. Appl. Microbiol. Biotechnol. 47:430-440.
32. Petersen, S., de Graaf, A.A., Eggeling, L., Mollney, M., Wiechert, W., and Sahm, H. 2000. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum. J. Biol. Chem. 275:35932-35941.
33. Petersen, S., Mack, C., de Graaf, A.A., Riedel, C., Eikmanns, B.J., and Sahm, H. 2001. Metabolic consequences of altered phosphoenolpyruvate carboxykinase activity in Corynebacterium glutamicum reveal anaplerotic regulation mechanisms in vivo. Metab. Eng. 3:344-361.
34. Peters-Wendisch, P.G., Eikmanns, B.J., Thierbach, G., Bachmann, B., and Sahm, H. 1993. Phosphoenolpruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production. FEMS Microbiol. Lett. 112:269-274.
35. Peters-Wendisch, P.G., Kreutzer, C., Kalinowski, J., Patek, M., Sahm, H., and Eikmanns, B.J. 1998. Pyruvate carboxylase from Corynebacterium glutamicum: Characterization, expression and inactivation of the pyc gene. Microbiology 144:915-927.
36. Peters-Wendisch, P.G., Schiel, B., Wendisch, V.F., Katsoulidis, E., Mockel, B., Sahm, H., and Eikmanns, B.J. 2001. Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. J. Mol. Microbiol. Biotechnol. 3:295-300.
37. Peters-Wendisch, P.G., Wendisch, V.F., Paul, S., Eikmanns, B.J., and Sahm, H. 1997. Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum. Microbiology 143:1095-1103.
38. 2-fixing enzyme, from Anaerobiospirillum succiniciproducens. J. Gen. Microbiol. 139:223-228.
39. Reinscheid, D.J., Eikmanns, B.J., and Sahm, H. 1994. Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme. J. Bacteriol. 176:3474-3483.
40. Riedel, C., Rittmann, D., Dangel, P., Mockel, B., Petersen, S., Sahm, H., and Eikmanns, B.J. 2001. Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production. J. Mol. Microbiol. Biotechnol. 3:573-583.
41. Ryu, H.W., Kang, K.H., Pan, J.G., and Chang, H.N. 2001. Characteristics and glycerol metabolism of fumarate-reducing Enterococcus faecalis RKY1. Biotechnol. Bioeng. 72:119-124.
42. Ryu, H.W., Kang, K.H., and Yun, J.S. 1999. Bioconversion of fumarate to succinate using glycerol as a carbon source. Appl. Biochem. Biotechnol. 77-79:511-520.
43. Salim, K., Haedens, V., Content, J., Leblon, G., and Huygen, K. 1997. Heterologous expression of the Mycobacterium tuberculosis gene encoding antigen 85A in Corynebacterium glutamicum. Appl. Environ. Microbiol. 63:4392-4400.
44. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Cold Spring Harbor Laboratory Press.
45. Sanger, F., Nicklen, S., and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
46. Schofield, G.M., and Schaal, K.P. 1981. A numerical taxonomic study of members of the Actinomycetaceae and related taxa. J. Gen. Microbiol. 127:237-259.
47. Stols, L., and Donnelly, M.I. 1997. Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant. Appl. Environ. Microbiol. 63:2695-2701.
48. Takahashi, N., and Yamada, T. 1992. Stimulatory effect of bicarbonate on the glycolysis of Actinomyces viscosus and its biochemical mechanism. Oral Microbiol. Immunol. 7:165-170.
49. Terasawa, M., Inui, M., Goto, M., Kurusu, Y., and Yukawa, H. 1991. Depression of by-product formation during L-isoleucine production by a living-cell reaction process. Appl. Microbiol. Biotechnol. 35:348-351.
50. Terasawa, M., Inui, M., Goto, M., Shikata, K., Imanari, M., and Yukawa, H. 1990. Living cell reaction process for L-isoleucine and L-valine production. J. Ind. Microbiol. 5:289-294.
51. Terasawa, M., Yukawa, H., and Takayama, Y. 1985. Production of L-aspartic acid from Brevibacterium by the cell re-using process. Process Biochem. 1:124-128.
52. Underwood, S.A., Buszko, M.L., Shanmugam, K.T., and Ingram, L.O. 2002. Flux through citrate synthase limits the growth of ethanologenic Escherichia coli KO11 during xylose fermentation. Appl. Environ. Microbiol. 68:1071-1081.
53. Uy, D., Delaunay, S., Engasser, J., and Goergen, J. 1999. A method for the determination of pyruvate carboxylase activity during the glutamic acid fermentation with Corynebacterium glutamicum. J. Microbiol. Methods 39:91-96.
54. Velick, S.F., and Furfine, C. 1963. Glyceraldehyde 3-phosphate dehydrogenase; in Lardy, H., and Myrbach, K. (eds.): The Enzymes. New York, Academic Press, vol. 7, pp. 243-273.
55. Vertès, A.A., Hatakeyama, K., Inui, M., Kobayashi, M., Kurusu, Y., and Yukawa, H. 1993a. Replacement recombination in coryneform bacteria: High efficiency integration requirement for non-methylated plasmid DNA. Biosci. Biotechnol. Biochem. 57:2036-2038.
56. Vertès, A.A., Inui, M., Kobayashi, M., Kurusu, Y., and Yukawa, H. 1993b. Presence of mrr- and mcr-like restriction systems in coryneform bacteria. Res. Microbiol. 144:181-185.
57. Wee, Y.J., Yun, J.S., Kang, K.H., and Ryu, H.W. 2002. Continuous production of succinic acid by a fumarate-reducing bacterium immobilized in a hollow-fiber bioreactor. Appl. Biochem. Biotechnol. 98-100:1093-1104.
58. Zeikus, J.G. 1980. Chemical and fuel production by anaerobic bacteria. Annu. Rev. Microbiol. 34:423-464.
59. Zeikus, J.G., Jain, M.K., and Elankovan, P. 1999. Biotechnology of succinic acid production and markets for derived industrial products. Appl. Microbiol. Biotechnol. 51:545-552.