Chapter 2 Electron Probe X-ray Microanalysis for the Study of Cell Physiology

Methods in Cell Biology

Volumn 88, Issue , 2008, Pages 19-43

  Fernandez Segura, E ^a   Warley, Alice ^b  

^a UNIVERSITY OF GRANADA   (Spain)

^b KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; BIOLOGICAL MODEL; CELL FUNCTION; CRYOPRESERVATION; ELECTRON PROBE MICROANALYSIS; HUMAN; INSTRUMENTATION; METHODOLOGY; REVIEW; SPECTROSCOPY;

ANIMALS; CELL PHYSIOLOGY; CRYOPRESERVATION; ELECTRON PROBE MICROANALYSIS; HUMANS; MODELS, BIOLOGICAL; SPECTRUM ANALYSIS;

EID: 46249124039     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0091-679X(08)00402-0     Document Type: Review

References (54)

1. Abraham E.H., Breslow J.L., Epstein J., Chang-Sing P., and Lechene C. Preparation of individual human diploid fibroblasts and study of ion transport. Am. J. Physiol. Cell Physiol. 248 (1985) C145-C164
2. Alaminos M., Sanchez-Quevedo M.C., Munoz-Avila J.I., Garcia J.M., Crespo P.V., Gonzalez-Andrades M., and Campos A. Evaluation of the viability of cultured corneal endothelial cells by quantitative electron-probe X-ray microanalysis. J. Cell. Physiol. 211 (2007) 692-698
3. Arrebola F., Canizares J., Cubero M.A., Crespo P.V., Warley A., and Fernandez-Segura E. Biphasic behaviour of changes in elemental composition during staurosporine- induced apoptosis. Apoptosis 10 (2005) 1317-1331
4. Arrebola F., Zabiti S., Canizares F.J., Cubero M.A., Crespo P.V., and Fernandez-Segura E. Changes in intracellular sodium,chlorine, and potassium concentrations in staurosporine-induced apoptosis. J. Cell. Physiol. 204 (2005) 500-507
5. Arrebola F., Fernandez-Segura E., Campos A., Crespo P.V., Skepper J.N., and Warley A. Changes in intracellular electrolyte concentrations during apoptosis induced by UV irradiation of human myeloblastic cells. Am. J. Physiol. Cell Physiol. 290 (2006) C638-C649
6. Borgmann S., Granitzer M., Crabbé J., Beck F.X., Nagel W., and Dörge A. Electron microprobe analysis of electrolytes in whole cultured eptithelial cells. Scanning Microsc. Suppl. 8 (1994) 139-148
7. Buja L.M., Hagler H.K., Parsons D.B., Chien K., Reynolds R.C., and Willerson J.T. Alterations of ultrastructure and elemental composition in cultured neonatal rat cardiac myocytes after metabolic inhibition with iodoacetic acid. Lab. Invest. 53 (1985) 397-412
8. Di Francesco A., Desnoyer R.W., Covacci V., Wolf F.I., Romani A., Cittadini A., and Bond M. Changes in magnesium content and subcellular distribution during retinoic acid-induced differentiation of HL60 cells. Arch. Biochem. Biophys. 360 2 (1998) 149-157
9. Dragomir A., and Roomans G.M. X-ray microanalysis of intestinal chloride transport in vitro and in vivo. Microsc. Res. Tech. 50 (2000) 176-181
10. Dragomir A., Andersson C., Aslund M., Hjelte L., and Roomans G.M. Assessment of chloride secretion in human nasal epithelial cells by X-ray microanalysis. J. Microsc. 203 (2001) 277-284
11. Edelman L. Optimal freeze-drying of cryosections and bulk specimens for X-ray microanalysis. Scanning Microsc. Suppl. 8 (1994) 67-81
12. Fernandez-Segura E., Canizares F.J., Cubero M.A., Revelles F., and Campos A. Backscattered electron imaging of cultured cells: Application to electron probe X-ray microanalysis using a scanning electron microscope. J. Microsc. 188 (1997) 72-78
13. Fernandez-Segura E., Canizares F.J., Cubero M.A., Campos A., and Warley A. A procedure to prepare cultured cells in suspension for electron probe X-ray microanalysis: Application to scanning and transmission electron microscopy. J. Microsc. 196 (1999) 19-25
14. Hall T.A. Quantitative electron probe X-ray microanalysis in biology. Scanning Microsc. 3 (1989) 461-466
15. Hall T.A., and Gupta B.L. EDS quantitation and application to biology. In: Joy D.C., Romig Jr. A.D., and Goldstein J.R. (Eds). Principles of Analytical Electron Microscopy (1986), Plenum Press, New York
16. Hardt M., and Plattman H. Quantitative energy-dispersive X-ray microanalayasis of calcium dynamics in cell suspensions during stimulation on a subsecond time scale: Preparation and analytical aspects as exemplified with Paramecium cells. J. Struct. Biol. 128 (1999) 187-199
17. Ingram P., Shelburne J.D., Roggli V.L., and LeFurgey A. Biomedical Applications of Microprobe Analysis (1999), Academic Press, San Diego, CA
18. Ingram P., Shelburne J.D., and LeFurgey A. Principles and instrumentation. In: Ingram P., Shelburne J., Roggli V., and LeFurgey A. (Eds). Biomedical Applications of Microprobe Analysis (1999), Academic Press, San Diego, CA 1-57
19. James-Kracke M.R., Sloane B.F., Shuman H., Karp R., and Somlyo A.P. Electron probe analysis of cultured vascular smooth muscle. J. Cell. Physiol. 103 (1980) 313-322
20. Laquerriere P., Banchet V., Michel J., Zierold K., Balsossier G., and Bonhomme P. X-ray microanalysis of organic thin sectionsin TEM using an UTW Si(Li) detector: Comparison of quantification methods. Microsc. Res. Tech. 52 (2001) 231-238
21. Leapman R.D. Detecting single atoms of calcium and iron in biological structures by electron energy-loss spectrum imaging. J. Microsc. 210 (2003) 5-15
22. LeFurgey A., Davilla S.D., Kopf D.A., Sommer J.R., and Ingram P. Real time quantitative elemental analysis and mapping: Microchemical imaging in cell physiology. J. Microsc. 165 (1992) 191-223
23. -. Comp. Biochem. Physiol. A 128 (2001) 385-394
24. LeFurgey A., Gannon M., Blum J., and Ingram P. Leishmania donovani amastigotes mobilize organic and inorganic osmolytes during regulatory volume decrease. J. Eukaryotic Microbiol. 52 (2005) 277-289
25. Marshall A.T. Light element X-ray microanalysis in Biology. Scanning Microsc. Suppl. 8 (1994) 187-201
26. Marshall A.T., and Patak A. Use of ultra-thin window detectors for biological microanalysis. Scanning Microsc. 7 (1993) 677-691
27. Nkamgeue E.M., Adnet J.-J., Bernard J., Zierold K., Kilian L., Jallot E., Benhayoune H., and Bonhomme P. In vitro effects of zirconia and alumina particles on human blood monocyte-derived macrophages: X-ray microanalysis and flow cytometric studies. J. Biomed. Mater. Res. 52 (2000) 587-594
28. Roomans G.M. The correction for extraneous background in quantitative X-ray microanalysis of biological thin sections: Some practical aspects. Scanning Microsc. 2 (1988) 311-318
29. Roomans G.M. Quantitative X-ray microanalysis of biological specimens. J. Electron Microsc Tech. 9 (1988) 19-43
30. Roomans G.M. X-ray microanalysis of epithelial cells in culture. In: Wise C. (Ed). Epithelial Cell Culture Protocols (2002), Humana Press, Totowa, NJ
31. Salido M., Vilches J., Lopez A., and Roomans G.M. X-ray microanalysis of etoposide-induced apoptosis in the PC-3 prostatic cancer cell line. Cell Biol. Int. 25 (2001) 499-508
32. Sanchez-Quevedo M.C., Alaminos M., Capitan L.M., Moreau G., Gorzon I., Crespo P.V., and campos A. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol. Histopathol. 22 (2007) 631-640
33. Scott D.A., Docampo R., Dvorak J.A., Shi S., and Leapman R.D. In situ compositional analysis of acidocalciosomes in Trypanosomi cruzi. J. Biol. Chem. 272 4 (1997) 28020-28029
34. Serretnyk Z., Krjukova J., Gaston B., Zaman K., Hjelte L., Roomans G.M., and Dragomir A. Activation of chloride transport in CF airway epithelial cell lines and primary CF nasal epithelial cells by S-nitrosoglutathione. Resp. Res. (2006) DOI: 10.11861/1645-9921-7-124
35. Simm C., Lahner B., Salt D., LeFurgey A., Ingram P., Yandell B., and Eide D.J. The yeast vacuole in zinc storage and intracellular zinc distribution. Eukaryotic Cell (2007) DOI: 10.1128/EC.00077-07
36. Sitte H., Neumann K., and Edelman L. Safety rules for cryopreparation. In: Steinbrecht R.A., and Zierold K. (Eds). Cryotechniques in Biological Electron Microscopy (1987), Springer-Verlag, Berlin, Heidelberg 285-289
37. Skepper J.N., Karydis I., Garnett M.R., Hegyi L., Hardwick S.J., Warley A., Mitchinson M.J., and Cary N.R.B. Changes in element concentrations are associated with early stages of apoptosis in human monocyte-macrophages exposed to oxidised low-density lipoprotein: An X-ray microanalysis study. J. Pathol. 188 (1999) 100-106
38. 2+, and other ions in rapidly frozen cells. Methods Enzymol. 172 (1989) 203-229
39. Statham P.J., and Pawley J.B. A new method for particle X-ray microanalysis based on peak to background measurements. Scanning Electron Microsc. I (1978) 469-477
40. Steinbrecht R.A., and Zierold K. A cryoembedding method for cutting ultrathin cryosections from small frozen specimens. J. Microsc. 136 (1984) 69-75
41. Warley A. Standards for the application of X-ray microanalysis to biological specimens. J. Microsc. 157 (1990) 135-147
42. Warley A. Quantitative X-ray microanalysis of thin sections in biology: Appraisal and interpretation of results. In: Sigee D.C., Morgan A.J., Sumner A.T., and Warley A. (Eds). X-ray Microanalysis in Biology. Experimental Techniques and Applications (1993), Cambridge University Press, Cambridge 47-57
43. Warley A. X-ray microanalysis for biologists. In: Glauert A.M. (Ed). Practical Methods in Electron Microscopy Vol. 16 (1997), Portland Press, London
44. Warley A. Potassium concentration is reduced in cultured rabbit tracheal smooth muscle cells after withdrawal of serum. Cell Biol. Int. 25 (2001) 691-695
45. Warley A., and Gupta B.L. Quantitative biological X-ray microanalysis. In: Harris J.R. (Ed). Electron Microscopy in Biology: A practical Approach (1991), IRL Press, Oxford 243-281
46. Warley A., and Skepper J.N. Long freeze-drying times are not necessary during the preparation of thin sections for X-ray microanalysis. J. Microsc. 198 (2000) 116-123
47. Warley A., Cracknell K.P.B., Cammish H.B., Twort C.H.C., Ward J.P.T., and Hirst S.J. Preparation of cultured airway smooth muscle for study of intracellular element concentrations by X-ray microanalysis:Comparisons of whole cells with cryosections. J. Microsc. 175 (1994) 143-145
48. Wendt-Gallitelli M.F., and Isenberg G. Total and free myoplasmic calcium during a contraction cycle: X-raymicroanalysis in guinea-pig ventricular myocytes. J. Physiol. 435 (1991) 349-352
49. Zhang B.-X., Desnoyer R.W., and Bond M. Extracellular adenosine triphosphate triggers arrythmias and element redistribution in electrically stimulated rat cardiac myocytes. Microsc. Microanal. 7 (2001) 48-55
50. Zierold K. X-ray microanalysis of freeze-dried and frozen-hydrated cryosections. J. Electron Microsc. Tech. 9 (1988) 65-82
51. Zierold K. Cryofixation methods for ion localization in cells by electron probe X-ray microanalysis: A review. J. Microsc. 161 (1991) 357-366
52. Zierold K. Effects of cadmium on electrolyte ions in cultured rat hepatocytes studied by X-ray microanalysis of cryosections. Toxicol. Appl. Pharmacol. 144 (1997) 70-76
53. Zierold K. Heavy metal cytotoxicity studied by electron probe X-ray microanalysis of cultured rat hepatocytes. Toxicology in vitro 14 (2000) 557-563
54. Zierold K., Michel J., Terryn C., and Balossier G. The distribution of light elements in biological cells measured by electron probe X-ray microanalysis of cryosections. Microsc. Microanal. 11 (2005) 138-145