Quantitative energy-dispersive X-ray microanalysis of calcium dynamics in cell suspensions during stimulation on a subsecond time scale: Preparative and analytical aspects as exemplified with Paramecium cells

Journal of Structural Biology

Volumn 128, Issue 2, 1999, Pages 187-199

  Hardt, Martin ^a   Plattner, Helmut ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

Calcium; Cilia; EDX; Electron microscopy; Exocytosis; Paramecium; Quenched flow; Secretion; X ray

Indexed keywords

CALCIUM;

ARTICLE; CALCIUM MOBILIZATION; CALCIUM TRANSPORT; CELL ACTIVATION; CELL MEMBRANE; CELL STIMULATION; CELL STRUCTURE; CELL SUSPENSION; CONTROLLED STUDY; CYTOSOL; NONHUMAN; PARAMECIUM; PRIORITY JOURNAL; X RAY MICROANALYSIS;

PARAMECIUM; PROTOZOA;

EID: 0033572328     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.1999.4188     Document Type: Article

References (47)

1. Adler E. M., Augustine G. J., Duffy S. N., Charlton M. P. Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse. J. Neurosci. 11:1991;1496-1507.
2. 2+ signalling requirements. Biochem. J. 337:1999;153-169.
3. Berridge M. J. Elementary and global aspects of calcium signalling. J. Physiol. 499:1997;291-306.
4. Berridge M. J. Neuronal calcium signaling. Neuron. 21:1998;13-26.
5. Berridge M. J., Bootman M. D., Lipp P. Calcium - A life and death signal. Nature. 395:1998;645-648.
6. 2+ buffers. Methods Cell Biol. 40:1994;3-29.
7. Bootman M. D., Berridge M. J. The elemental principles of calcium signaling. Cell. 83:1995;675-678.
8. Buchanan R. A., Leapman R. D., O'Connell M. F., Reese T. S., Andrews S. B. Quantitative scanning transmission electron microscopy of ultrathin cryosections: Subcellular organelles in rapidly frozen liver and cerebellar cortex. J. Struct. Biol. 110:1983;244-255.
9. Chandler J. A. A method for preparing absolute standards for quantitative calibration and measurement of section thickness with X-ray microanalysis of biological ultrathin specimens in EMMA. J. Microsc. 106:1976;291-302.
10. DeBrujin W. C. Ideal standards for quantitative X-ray microanalysis of biological specimens. Scanning Electron Microsc. 1981-II:1981;357-367.
11. Dörge A., Rick R., Gehring K., Thurau K. Preparation of freeze-dried cryosections for quantitative X-ray microanalysis in biological soft tissues. Pflügers Arch. Eur. J. Physiol. 373:1978;85-97.
12. 2+ release from subplasmalemmal stores as a primary event during exocytosis in Paramecium cells. J. Cell Biol. 127:1994;935-945.
13. Goodenough R. D., Stenger V. A. Magnesium, calcium, strontium, barium and radium. Bailar J. C. Comprehensive Inorganic Chemistry. 1973;591-664 Pergamon, Oxford.
14. Hall T. A. Biological X-ray microanalysis. J. Microsc. 117:1979;145-163.
15. 2+ transients induced in Paramecium cells by a polyamine secretagogue. J. Cell Sci. 110:1997;975-983.
16. 2+ diffusion near the membrane: Implications for secretion in neuroendocrine cells. Biophys. J. 72:1997;674-690.
17. Knoll G., Braun C., Plattner H. Quenched flow analysis of exocytosis in Paramecium cells: Time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells. J. Cell Biol. 113:1991;1295-1304.
18. Knoll G., Grässle A., Braun C., Probst W., Höhne-Zell B., Plattner H. A calcium influx is neither strictly associated with nor necessary for exocytotic membrane fusion in Paramecium cells. Cell Calcium. 14:1993;173-184.
19. 2+ stores of probable relevance for exocytosis in Paramecium. Alveolar sacs share some but not all characteristics with sarcoplasmic reticulum. Cell Calcium. 17:1995;335-344.
20. Machemer H. Electrophysiology. Görtz H. D. Paramecium. 1988;185-215 Springer-Verlag, Berlin/Heidelberg.
21. 2+-release channel function. Biochem. J. 337:1999;345-361.
22. 2+ store: A view from the lumen. Trends Biochem. Sci. 23:1998;10-14.
23. 2+ binding in bovine chromaffin cells. Biophys. J. 75:1998;1635-1647.
24. Neher E. Concentration profiles of intracellular calcium in the presence of a diffusible chelator. Exp. Brain Res. 14:1986;80-96.
25. 2+ microdomains: New tools for understanding their roles in neurotransmitter release. Neuron. 20:1998;389-399.
26. Nicaise G., Maggio K., Thirion S., Horoyan M., Keicher E. The calcium loading of secretory granules. A possible key event in stimulus-secretion coupling. Biol. Cell. 75:1992;89-99.
27. Nuccitelli R. A Practical Guide to the Study of Calcium in Living Cells. 1994;Academic Press, San Diego/New York/Boston.
28. Plattner H., Bachmann L. Cryofixation: A tool in biological ultrastructural research. Int. Rev. Cytol. 79:1982;237-304.
29. Plattner H., Braun C., Hentschel J. Facilitation of membrane fusion during exocytosis and exocytosis-coupled endocytosis and acceleration of "ghost" detachment in Paramecium by extracellular calcium. A quenched-flow/freeze-fracture analysis. J. Membr. Biol. 158:1997a;197-208.
30. Plattner H., Habermann A., Kissmehl R., Klauke N., Majoul I., Söling H. D. Differential distribution of calcium stores in Paramecium cells. Occurrence of a subplasmalemmal store with a calsequestrin-like protein. Eur. J. Cell Biol. 71:1997b;297-306.
31. Plattner H., Knoll G., Erxleben C. The mechanics of biological membrane fusion. Merger of aspects from electron microscopy and patch-clamp analysis. J. Cell Sci. 103:1992;613-618.
32. Plattner H., Knoll G., Pape R. Synchronization of different steps of the secretory cycle in Paramecium tetraurelia: Trichocyst exocytosis, exocytosis-coupled endocytosis, and intracellular transport. Plattner H. Membrane Traffic in Protozoa. 1993;123-148 JAI Press, London/Greenwich.
33. Plattner H., Lumpert C. J., Knoll G., Kissmehl R., Höhne B., Momayezi M., Glas-Albrecht R. Stimulus-secretion coupling in Paramecium cells. Eur. J. Cell Biol. 55:1991;3-16.
34. Plattner H., Matt H., Kersken H., Haacke B., Stürzl R. Synchronous exocytosis in Paramecium cells. I. A novel approach. Exp. Cell Res. 151:1984;6-13.
35. Plattner H., Stürzl R., Matt H. Synchronous exocytosis in Paramecium cells. IV. Polyamino compounds as potent trigger agents for repeatable trigger-redocking cycles. Eur. J. Cell Biol. 36:1985;32-37.
36. Plattner H., Zingsheim H. P. Electron microscopic methods in cellular and molecular biology. Subcell. Biochem. 9:1983;1-261.
37. Poenie M., Epel D. Ultrastructural localization of intracellular calcium stores by a new cytochemical method. J. Histochem. Cytochem. 35:1987;939-956.
38. Reimer L. Scanning Electron Microscopy: Physics of Image Formation and Microanalysis, 2nd ed. 1998;Springer-Verlag, Berlin/Heidelberg/New York.
39. Roos N., Barnard T. A comparison of subcellular element concentrations in frozen-dried, plastic-embedded, dry-cut sections and frozen-dried cryosections. Ultramicroscopy. 17:1985;335-344.
40. Schmitz M., Zierold K. X-ray microanalysis of ion changes during fast processes of cells, as exemplified by trichocyst exocytosis of Paramecium caudatum. Plattner H. Electron Microscopy of Subcellular Dynamics. 1989;325-339 CRC Press, Boca Raton.
41. Sitte H. Advanced instrumentation and methodology related to cryoultramicrotomy: A review. Scanning Microsc. Suppl. 10:1996;387-466.
42. Somlyo A. V., Gonzales-Serratos H., Shuman H., McClellan G., Somlyo A. P. Calcium release and ion changes in the sarcoplasmic reticulum of tetanized muscle: An electron-probe study. J. Cell Biol. 90:1981;577-594.
43. Stelly N., Mauger J. P., Keryer G., Claret M., Adoutte A. Cortical alveoli of Paramecium: A vast submembraneous calcium storage compartment. J. Cell Biol. 113:1991;103-112.
44. Verkhratsky A., Toescu E. C. Integrative Aspects of Calcium Signalling. 1998;Plenum, New York/London.
45. 2+ stores in invertebrate photoreceptors. Cell Calcium. 18:1995;342-351.
46. Warley A. X-Ray Microanalysis for Biologists. 1997;Portland Press, London/Miami.
47. Wendt-Gallitelli M. F., Isenberg G. Total and free myoplasmic calcium during a contraction cycle: X-ray microanalysis in guinea-pig ventricular myocytes. J. Physiol. 435:1991;349-372.