A procedure to prepare cultured cells in suspension for electron probe X-ray microanalysis: Application to scanning and transmission electron microscopy

Journal of Microscopy

Volumn 196, Issue 1, 1999, Pages 19-25

  Fernández Segura, Eduardo ^a   Canizares F J ^a   Cubero, M A ^a   Campos, A ^a   Warley, A ^b  

^a UNIVERSITY OF GRANADA   (Spain)

^b ST THOMAS HOSPITAL   (United Kingdom)

Author keywords

Cell culture; Electron probe X ray microanalysis; Potassium; Scanning electron microscope; Scanning transmission electron microscope; Sodium

Indexed keywords

CELLS; ELECTRON PROBE MICROANALYSIS; ELECTRONS; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; POTASSIUM; PROBES; SCANNING ELECTRON MICROSCOPY; SODIUM; WASHING;

ACCELERATING VOLTAGES; CULTURED CELL; DISTILLED WATER; ELECTRON PROBE X RAY MICROANALYSIS; ELEMENTAL CONTENTS; SCANNING ELECTRON MICROSCOPE; SCANNING ELECTRONS; SCANNING MODE; SCANNING TRANSMISSION ELECTRON MICROSCOPES; WASHING SOLUTION;

CELL CULTURE;

ARTICLE; CELL CULTURE; CRYOPRESERVATION; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; IMAGE ANALYSIS; MICROANALYSIS; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

EID: 0032883018     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1999.00588.x     Document Type: Article

References (32)

1. Abraham, E.H., Brewslow, J.L., Epstein, J., Chang-Sing, P. & Lechene, C. (1985) Preparation of individual human diploid fibroblasts and study of ion transport. Am. J. Physiol. 248, C154-C164.
2. Boekestein, A., Thiel, F., Stols, A.L.H., Bouw, E. & Stadhouders, A.M. (1984) Surface roughness and the use of a peak to background ratio in the X-ray microanalysis of bio-organic bulk specimens. J. Microsc. 134, 327-333.
3. Borgmann, S., Granitzer, M., Crabbé, J., Beck, F.X., Nagel, W. & Dörge, A. (1994) Electron microprobe analysis of electrolytes in whole cultured epithelial cells. Scanning Microsc. Suppl. 8, 139-148.
4. Buja, L.M., Hagler, H.K., Parsons, D., Chien, K., Reynolds, R.C. & Willerson, J.T. (1985) Alterations of ultrastructure in cultured neonatal rat cardiac myocytes after metabolic inhibition with iodoacetic acid. Lab. Invest. 53, 397-412.
5. Cameron, L.L., Hansen, J.T., Hunter, K.E. & Padilla, G.M. (1986) Elemental concentration gradients between subcellular compartments. J. Cell Sci. 80, 283-297.
6. Echlin, P. & Taylor, S.E. (1986) The preparation and X-ray microanalysis of bulk frozen hydrated vacuolate plant tissue. J. Microsc. 141, 329-412.
7. Fernández-Segura, E., Cañizares, F.J., Cubero, M.a., Revelles, F. & Campos, A. (1997a) Backscattered electron imaging of cultured cells: electron probe X-ray microanalysis using a scanning electron microscope. J. Microsc. 188, 72-78.
8. Fernández-Segura, E., Cañizares, F.J., Cubero, M.A., Revelles, F. & Campos, A. (1997b) Electron probe X-ray microanalysis of cultured epithelial tumor cells with scanning electron microscopy. Cell Biol. Int. 21, 665-669.
9. Hall, S.R., Sigee, D.C. & Beesley, J.E. (1992) Scanning X-ray microanalysis of microcarrier cultured endothelial cells: elemental changes during the transition to confluency and the effect of ionophore A23187. Scanning Microsc. 6, 753-763.
10. Hongpaisan, J., Zhang, A.-L., Mörk, A.-C. & Roomans, G. (1996) Use of primary cell cultures and intact isolated glandular epithelia for X-ray microanalysis. J. Microsc. 184, 22-34.
11. James-Kracke, M.R., Sloane, B.E., Shuman, H., Karp, K. & Somlyo, A.P. (1980) Electron probe analysis of cultured vascular smooth muscle. J. Cell Physiol. 103, 313-322.
12. Larsson, L., Aperia, A. & Lechene, C. (1986) Ionic transport in individual renal epithelial cells from adult and young rats. Acta Physiol. Scand. 126, 321-332.
13. Lechene, C. (1988) Electron probe analysis of transport properties of cultured cells. Electron Microanslysis. Applications in Biology and Medicine (ed. by K. Zierold, H. K. Hagler), pp. 237-249. Springer, Heidelberg.
14. López-Escamez, J.A. & Campos, A. (1994) Standards for X-ray microanalysis of calcified structures. Scanning Microsc. Suppl. 8, 171-185.
15. Rick, R., Dörge, A., Bauer, R., Gehring, K. & Thurau, K. (1979) Quantification of electrolytes in freeze-dried cryosections by electron microprobe analysis. Scanning Electron Microsc. II, 619-626.
16. Roomans, G.M. (1979) Standards for X-ray microanalysis of biological specimens. Scanning Electron Microsc. II, 649-657.
17. Roomans, G.M. (1988) Quantitative X-ray microanalysis of biological specimens. J. Electron Microsc. Tech. 9, 19-44.
18. Roomans, G.M., Hongpaisan, J., Jin, Z., Mork, A.C. & Zhang. A. (1996) In vitro systems and cultured cells as specimens for X-ray microanalysis. Scanning Microsc. Suppl. 10, 359-370.
19. Statham, P. & Pawley, J.B. (1978) A new method for particle X-ray microanalysis based on peak-to-background measurements. Scanning Electron Microsc. I, 469-478.
20. Tvedt, K.E., Halgunset, J., Kopstad, G. & Haugen, O.A. (1988) Quick sampling and perpendicular cryosectioning of cell monolayers for the X-ray microanalysis of diffusible elements. J. Microsc. 151, 49-59.
21. von Euler, A., Pâlsgârd, E., Vult von Steyeren, C. & Romans, G.M. (1993) X-ray microanalysis of epithelial and secretory cells in culture. Scanning Microsc. 7, 191-202.
22. Warley, A. (1986) Elemental concentrations in isolated rat thymocytes prepared for cryofixation in the presence of different media. J. Microsc. 144, 183-191.
23. Warley, A. (1988) X-ray microanalysis of freshly isolated cells in suspension. Electron Probe Microanalysis. Application in Biology and Medicine (ed. by K. Zierold, H. K. Hagler), pp. 169-179. Springer, Heidelberg.
24. Warley, A. (1990) Standards for the application of X-ray microanalysis to biological specimens. J. Microsc: 157, 135-147.
25. Warley, A. (1994) The preparation of cultured cells for X-ray microanalysis. Scanning Microsc. Suppl. 8, 129-138.
26. 3 cells infected with vaccinia virus. J. Cell Sci. 75, 401-409.
27. Warley, A., Cracknell, K.P.B., Cammish, H.B., Twort, C.H.C., Ward, J.P.T. & Hirst, S.J. (1994) Preparation of cultured airway smooth muscle for study of intracellular element concentrations by X-ray microanalysis: comparison of whole cells with cryosections. J. Microsc. 175, 143-153.
28. Wroblewski, J. & Wroblewski, R. (1991) X-ray microanalysis of cultured mammalian cells. X-ray Microanalysis in Biology: Experimental Techniques and Applications (ed. by D. C. Sigee, A. J. Morgan, A. T. Summer and A. Warley), pp. 317-329. Cambridge University Press, Cambridge.
29. Zhang, A.L. & Roomans, G.M. (1997a) Ion transport in cultured pig tracheal submucosal gland acinar cells studied by X-ray microanalysis. Eur. Respir. J. 10, 2204-2209.
30. 2A adrenoreceptors in HT29 cells. Cell Biol. Int. 21, 195-201.
31. Zhang, W. & Roomans, G.M. (1998) Volume-induced chloride transport in HT29 cells studied by X-ray microanalysis. Microsc. Res. Tech. 40, 72-78.
32. Zierold, K. & Schäfer, D. (1988) Preparation of cultured and isolated cells for X-ray microanalysis. Scanning Microsc. 2, 1775-1779.