High-throughput screening of chromatographic separations: I. method development and column modeling

Biotechnology and Bioengineering

Volumn 100, Issue 4, 2008, Pages 605-618

  Coffman, Jonathan L ^a   Kramarczyk, Jack F ^a   Kelley, Brian D ^a,b  

^a PFIZER INC   (United States)

^b GENENTECH INC   (United States)

Author keywords

Batch binding; Chromatography; Column modeling; High throughput screening; Method development

Indexed keywords

(E ,3E) PROCESS; (I ,J) CONDITIONS; 96 WELL; ANION EXCHANGES; BIOPHARMACEUTICALS; CHROMATOGRAPHIC COLUMNS; CHROMATOGRAPHIC PURIFICATION; CHROMATOGRAPHIC RESINS; CHROMATOGRAPHIC SEPARATIONS; CONTACT TIME; FILTER PLATES; HIGH THROUGHPUT SCREENING (HTS); HYDROPHOBIC INTERACTIONS; INCUBATION TIME; KINETICS AND THERMODYNAMICS; METHOD DEVELOPMENT; PARAMETER SPACES; PROTEIN PURIFICATION; PURIFICATION PROCESSES; REPRODUCIBILITY;

CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; COLUMNS (STRUCTURAL); FLOW INTERACTIONS; FLUIDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY; MATERIALS HANDLING; MIXING; OPTIMIZATION; PURIFICATION; SEPARATION; THEOREM PROVING; THROUGHPUT;

RESINS;

AGAROSE; CYCLIC GMP; HYDROXYAPATITE; IMMUNOGLOBULIN FC FRAGMENT; MONOCLONAL ANTIBODY; POLY(METHYL METHACRYLATE); RESIN; SEPHAROSE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CENTRIFUGATION; CERAMICS; CHROMATOGRAPHIC COLUMN; DEVICE; ELUTION; EVALUATION; FILTER; HIGH THROUGHPUT SCREENING; INCUBATION TIME; KINETICS; LIQUID; PARAMETER; PERFORMANCE; PIPETTE; PROCESS OPTIMIZATION; PROTEIN PURIFICATION; REPRODUCIBILITY; ROBOTICS; THERMODYNAMICS;

CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; FILTRATION; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; ION EXCHANGE RESINS; KINETICS; PROTEOMICS; RECOMBINANT FUSION PROTEINS; RESEARCH;

EID: 46249092863     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21904     Document Type: Article

References (37)

1. Albala JS, Franke K, McConnell IR, Pak KL, Folta PA, Rubinfeld B, Davies AH, Lennon GG, Clark R. 2000. From genes to proteins: high-throughput expression and purification of the human proteome. J Cell Biochem 80(2):187-191.
2. Brumbaugh EE, Ackers GK. 1971. Molecular sieve studies of interacting protein systems. VII. Direct optical scanning method for ligand-macromolecule binding studies. Anal Biochem 41(2):543-559.
3. Chapman T. 2005. Pure but not simple. Nature 434:795-798.
4. Charlton H, Galarza B, Leriche K, Jones R. 2006. Chromatography process development using 96-well microplate formats. BioPharm (June Supp):20-26, 42.
5. Cramer SM, Rege K, Dordick JS. 2001. Feb. 23, 2001. High Throughput Screening of Potential Displacer Molecules. US. patent application 09/ 791,317.
6. Emili AQ, Cagney G. 2000. Large-scale functional analysis using peptide or protein arrays. Nat Biotechnol 18(4):393-397.
7. Garr CD, Schultz L, Cameron LM. 2004. Purification and analysis of parallel libraries. In: Kyranos IN, editor. Purification and Analysis of Parallel libraries. Amsterdam: Elsevier.
8. Graham EE, Fook CF. 1982. Rate of protein absorption and desorption on cellulosic ion exchangers. AIChE J 28(2):245-250.
9. Hubbuch J, Linden T, Knieps E, Thommes J, Kula M-R. 2002. Dynamics of protein uptake within the adsorbent particle during packed bed chromatography. Biotechnol Bioeng 80(4):359-368.
10. Kelley BD, Switzer M, Bastek P, Kramarczyk JF, Yu T, Coffman JC. 2008. High-throughput screening of chromatographic separations: 4. Ion Exchange. Biotechnol Bioeng, in press.
11. Kramarczyk JF. 2003. High-Throughput Screening of Chromatographic Resins and Excipients for Optimizing Selectivity. Medford, MA: Tufts University.
12. Kramarczyk JF, Kelley BD, Coffman JC. 2008. High-throughput screening of chromatographic separations: II. Hydrophobic Interaction. Biotechnol Bioeng 100:707-720.
13. Lacki KM, Bergander T, Gronberg A, Oberg K. 2007. High throughput process development: Determination of dynamic binding capacity using microtiter plates filled with chromatography resins. Abstracts of Papers, 234th ACS National Meeting. Boston, MA, United States: August 19-23, 2007:BIOT-470.
14. Lesley SA. 2001. High-throughput proteomics: protein expression and purification in the postgenomic world. Protein Expr Purif 22(2): 159-164.
15. Li R, Dowd V, Stewart DJ, Burton SJ, Lowe CR. 1998. Design, synthesis, and application of a protein A mimetic. Nat Biotechnol 16(2):190-195.
16. Linden T, Ljunglof A, Kula M-R, Thommes J. 1999. Visualizing two-component protein diffusion in porous adsorbants by confocal scanning laser microscopy. Biotechnol Bioeng 65(6):622-630.
17. Liu J, Yang T, Ladiwala A, Cramer SM, Breneman CM. 2006. High throughput determination and QSER modeling of displacer DC 50 values for ion exchange systems. Sep Sci Technol 41(14):3079-3107.
18. Lowe CR. 2001. Combinatorial approaches to affinity chromatography. Curr Opin Chem Biol 5(3):248-256.
19. Mazza CB, Rege K, Breneman CM, Sukumar N, Dordick JS, Cramer SM. 2002a. High-throughput screening and quantitative structure-efficacy relationship models of potential displacer molecules for ion-exchange systems. Biotechnol Bioeng 80(1):60-72.
20. Mazza CB, Rege K, Breneman CM, Sukumar N, Dordick JS, Cramer SM. 2002b. High-throughput screening and quantitative structure-efficacy relationship models of potential displacer molecules for ion-exchange systems. Biotechnol Bioeng 80(1):60-72.
21. Merson JR. Pfizer Lt, assignee. 1991. An Assay Tray Assembly. patent application 91303083.9.
22. Pottorf RS, Player MR. 2004. Process technologies for purity enhancement of large discovery libraries. Curr Opin Drug Discov Devel 7(6):777-783.
23. Rege K, Tugcu N, Cramer SM. 2003. Predicting column performance in displacement chromatography from high throughput screening batch experiments. Sep Sci Technol 38(7):1499-1517.
24. Rege K, Ladiwala A, Cramer SM. 2005a. Multidimensional high-throughput screening of displacers. Anal Chem 77:6818-6827.
25. Rege K, Pepsin M, Flacon B, Steele L, Heng M. 2005b. High-throughput process development for recombinant protein purification. Biotechnol Bioeng 93(4):618-630.
26. Saraswat LD, Zhang H, Hardy LW, Jones SS, Bhikhabhai R, Brink C, Bergenstrahle A, Haglund R, Gallion SL. 2005. Affinity ligand selection from a library of small molecules: assay development, screening, and application. Biotechnol Prog 21(1):300-308.
27. Scopes R. 1987. Protein Purification: Principles and Practice. New York: Springer.
28. Shepard CC, Tiselius A. 1949. The chromatography of proteins. The effect of salt concentration and pH on the adsorption of proteins to silica gels. Discuss. Faraday Soc. 7:275-285.
29. Stein A, Kiesewetter A. 2006. Cation exchange chromatography in antibody purification: pH optimized screening for optimized binding and HCP removal. J. Chromatogr. B 848:151-158.
30. Sunasara KM, Xia F, Gronke RS, Cramer SM. 2003. Application of hydro-phobic interaction displacement chromatography for an industrial protein purification. Biotechnol Bioeng 82(3):330-339.
31. Tecan. 2008. RSP Operating Manual Report nr 390 783 V2.
32. Thiemann J, Jankowski J, Rykl J, Kurzawski S, Pohl T, Wittmann-Liebold B, Schluter H. 2004. Principle and application of the protein-parameter screening system. J. Chromatogr. A 1043:73-80.
33. Venn RF, Merson JR, Cole S, Macrae P. 2004. 96-well solid-phase extraction: A brief history of its development. J. Chromatogr. B 817:77-80.
34. Wenger MD, DePhillips P, Price CE, Bracewell DG. 2007. An automated microscale chromatographic purification of virus-like particles as a strategy for process development. Biotechnol Appl Biochem 47:131-139.
35. Wensel DL, Kelley BD, Coffman JC. 2008. High-throughput screening of chromatographic separations: 3. Monoclonal Antibodies on Hydro-xyapatite. Biotechnol Bioeng, in press.
36. Wierling PS, Bogumil R, Knieps-Grünhagen E, Hubbuch J. 2007. High-throughput screening of packed-bed chromatography coupled with SELDI-TOF MS analysis: Monoclonal antibodies versus host cell protein. Biotechnol Bioeng 98(2):440-450.
37. Yoshida H, Yoshikawa M, Kataoka T. 1994. Parallel transport of BSA by surface and pore diffusion in strongly basic chitosan. AIChE J 40(12): 2034-2044.