Affinity ligand selection from a library of small molecules: Assay development, screening, and application

Biotechnology Progress

Volumn 21, Issue 1, 2005, Pages 300-308

  Saraswat, Lakshmi D ^a   Zhang, Huiyun ^a   Hardy, Larry W ^b   Jones, Simon S ^a   Bhikhabhai, Rama ^c   Brink, Charlotte ^c   Bergenstråhle, Annika ^c   Haglund, Rolf ^c   Gallion, Steven L ^a  

^a ARQULE INC   (United States)

^b Aurigene Discovery Technologies   (United States)

^c GE Healthcare Bio Sciences AB   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; COST EFFECTIVENESS; FILTERS (FOR FLUIDS); FLUORESCENCE; LEAD COMPOUNDS;

AFFINITY LIGANDS; DYNAMIC BINDING CAPACITY; FLUORESCAMINES; HUMAN SERUM ALBUMUIN (HSA);

PROTEINS;

LIGAND; SERUM ALBUMIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; COMBINATORIAL CHEMISTRY; ECONOMICS; HUMAN; METHODOLOGY; PHYSIOLOGY; PROTEIN BINDING; SENSITIVITY AND SPECIFICITY;

CHROMATOGRAPHY, AFFINITY; COMBINATORIAL CHEMISTRY TECHNIQUES; HUMANS; LIGANDS; PROTEIN BINDING; SENSITIVITY AND SPECIFICITY; SERUM ALBUMIN;

EID: 13544249733     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp040019w     Document Type: Article

References (34)

1. Scouten, W. H. Affinity Chromatography, Bio-Selective Adsorption in Inert Matrices; Wiley: New York, 1981.
2. Travis, J.; Pannell, R. Selective removal of albumin from plasma by affinity chromatography. Clin. Chim. Acta 1973, 49 (1), 49-52.
3. Porath, J.; Maisano, F.; Belew, M. Thiophilic adsorption-a new method for protein fractionation. FEBS Lett. 1985, 185 (2), 306-10.
4. El-Kak, A.; Vijaylakshmi, M. A. Study of the separation of mouse monoclonal antibodies by pseudobioaffinity chromatography using matrix-linked histidne and histamine. J. Chromatogr., B: Biomed. Sci. Appl. 1991, 570, 29-41.
5. Li, R.; Dowd, V.; Stewart, D. J.; Burton, S. J.; Lowe, C. R. Design, synthesis, and application of a protein A mimetic. Nat. Biotechnol. 1998, 16 (2), 190-195.
6. Ngo, T. T.; Khatter, N. Chemistry and preparation of affinity ligands useful in immunoglobulin isolation and serum protein separation. J. Chromatogr. 1990, 510, 281-91.
7. Drews, J. Genomics sciences and the medicine of tomorrow. Nat. Biotechnol. 1996, 14, 1516-1518.
8. Ehrlich, G. K.; Bailon, P.; Berthold, W. Phage display technology: Identification of peptides as model ligands for affinity chromatography. Methods Mol. Biol. 2000, 147, 209-220.
9. Melissis, S. C.; Rigden, D. J.; Clonis, Y. D. New family of glutathionyl-biomimetic ligands for affinity chromatography of glutathione-recognizing enzymes. J. Chromatogr., A 2001, 917, 29-42.
10. Ehrlich, G. K.; Bailon, P. Identification of model peptides as affinity ligands for the purification of humanized monoclonal antibodies by means of phage display. J. Biochem. Biophys. Methods 2001, 49, 443-454.
11. Verdoliva, A.; Basile, G.; Fassina, G. Affinity purification of immunoglobulins from chicken egg yolk using a new synthetic ligand. J. Chromatogr., B: Biomed. Sci. Appl. 2000, 749, 233-242.
12. Teng, S. F.; Sproule, K.; Husain, A.; Lowe, C. R. Affinity chromatography on immobilized "biomimetic" ligands. Synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand. J. Chromatogr., B: Biomed. Sci. Appl. 2000, 740 (1), 1-15.
13. Sproule, K.; Morill, P.; Pearson, J. C.; Burton, S. J.; Hejnaes, K. R.; Valore, H.; Ludvigsen, S.; Lowe, C. R. New strategy for the design of ligands for the purification of pharmaceutical proteins by affinity chromatography. J. Chromatogr., B: Biomed. Sci. Appl. 2000, 740 (1), 17-33.
14. Gurgel, P. V.; Carbonell, R. G.; Swaisgood, H. E. Identification of peptide ligands generated by combinatorial chemistry that bind alpha-lactalbumin. Sep. Sci. Technol. 2001, 36 (11), 2411-2431.
15. Fassina, G.; Ruvo, M.; Palombo, G.; Verdoliva, A.; Marino, M. Novel ligands for the affinity-chromatographic purification of antibodies. J. Biochem. Biophys. Methods 2001, 49, 481-490.
16. Wang, Y.; Li, T. Identification of affinity ligands for protein purification from synthetic chemical combinatorial libraries. Biotechnol. Prog. 2002, 18, 524-529.
17. Liang, R. et al., Parallel synthesis and screening of a solid-phase carbohydrate library. Science 1996, 274, 1520-1522.
18. Czarnik, A.; Keene, J. D. Combinatorial chemistry. Curr. Biol. 1998, 8, R705-R707.
19. Regberg, T.; Lindquist, C.; Pilotti, A.; Ellström, C.; Eckersten, A.; Fägerstam, L.; Shinohara, Y.; Öberg, K.; Gallion, S. L.; Cheng Tu, C.; Hogan, J. C. Discovery of novel affinity ligands using structure-guided, high-throughput combinatorial chemistry. J. Chromatogr. Submitted for publication.
20. Lenz, G. R.; Nash, H. M.; Jindal, S. Chemical ligands, genomics and drug discovery. Drug Discovery Today 2000, 5 (4), 145-156.
21. Gordon, E. M.; Barrett, R. W.; Dower, W. J.; Fodor, S.; Gallop, M. A. Applications of combinatorial technologies to drug discovery. 2. Combinatorial organic synthesis, library screening strategies, and future directions. J. Med. Chem. 1994, 37 (10), 1385-1401.
22. Gallop, M. A.; Barrett, R. W.; Dower, W. J.; Fodor, S.; Gordon, E. M. Applications of combinatorial technologies to drug discovery. 1. Background and peptide combinatorial libraries. J. Med. Chem. 1994, 37 (9), 1233-1251.
23. Roque, A. C.; Taipa, M. A.; Lowe, C. R. A new method for the screening of solid-phase combinatorial libraries for affinity chromatography. J. Mol. Recognit. 2004, 17 (3), 262-7.
24. Rajur, S. B.; Johnson, A.; Varady, L. Combinatorial synthesis of N-substituted α-amino acids on Sepharose. In Innovations and Perspectives in Solid-Phase Synthesis and Combinatorial Libraries 2000; Epton, R., Ed.; Mayflower Worldwide: Kingswinford, England, 2001; pp 105-108.
25. Ditto, L.; Nicewonger, R.; Varady, L. Combinatorial synthesis of a benzimidazole array on Sepharose. Abstracts of Papers, 219th National Meeting of the American Chemical Society; American Chemical Society: Washington, DC, 2000; ORGN 176.
26. Varady, L.; Rajur, S. B.; Nicewonger, R. B.; Guo, M. J.; Ditto, L.; Johnson, A.; Saraswat, L.; Zhang, H.; Rooney, M.; Gallion, S. Design, synthesis and screening of combinatorial libraries on Sepharose 4FF. Presented at the 19th International Symposium on the Separation of Proteins, Peptides, and Polynucleotides, Delray Beach, Florida, 1999
27. Kessler, M. A.; Meinitzer, A.; Wolfbeis, O. S. Albumin blue 580 fluorescence assay for albumin. Anal. Biochem. 1997, 248, 180-182.
28. Peters, T. All about Albumin; Academic Press: New York, 1996; p 25.
29. Nishikata, M.; Ishii, S.-I. Frontal chromatography: Theory for its application to studies on specific interactions of biomolecules. J. Chromatogr. 1986, 376, 33-47.
30. Cohn, E. J. J. Am. Chem. Soc. 1946, 68, 459.
31. Fogel, P.; Collette, P.; Dupront, A.; Garyantes, T.; Guedin, D. The confirmation rate of primary hits: A predictive model. J Biomol. Screening 2002, 7 (3), 175-190.
32. Kragh-Hansen, U. Molecular aspects of ligand binding to serum albumin. Pharmacol. Rev. 1981, 33, 17-53.
33. Berglöf, E.; Curling, J. M. Chromatographic preparation and in vitro properties of albumin from human plasma. J. Appl. Biochem. 1983, 5, 282-2934.
34. Curling, J. M. Albumin purification by ion exchange chromatography. In Methods of Plasma Protein Fractionation; Curling, J. M., Ed.; Academic Press: New York, 1980; pp 78-91.