Ethanol extract of Dunaliella salina induces cell cycle arrest and apoptosis in A549 human non-small cell lung cancer cells

In Vivo

Volumn 22, Issue 3, 2008, Pages 369-378

  Sheu, Ming Jyh ^a   Huang, Guan Jhong ^a   Wu, Chieh Hsi ^a   Chen, Jwo Sheng ^a   Chang, Heng Yuan ^a   Chang, Shu Jen ^a   Chung, Jing Gung ^a,b  

^a CHINA MEDICAL UNIVERSITY   (Taiwan)

^b ASIA UNIVERSITY   (Taiwan)

Author keywords

A549; Apoptosis; Dunaliella salina; NSCLS; p53; carotene

Indexed keywords

9 CIS BETA CAROTENE; ALCOHOL; ALL TRANS BETA CAROTENE; ALPHA CAROTENE; BETA CAROTENE; CHLOROPHYLL; CYCLIN DEPENDENT KINASE; DEATH RECEPTOR; DUNALIELLA SALINA EXTRACT; FAS ANTIGEN; FAS LIGAND; LIPOCORTIN 5; LYCOPENE; NATURAL PRODUCT; PROPIDIUM IODIDE; PROTEIN BAX; PROTEIN P21; PROTEIN P53; XANTHOPHYLL; ZEAXANTHIN;

APOPTOSIS; ARTICLE; CELL CYCLE ARREST; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CHEMICAL COMPOSITION; CONTROLLED STUDY; DRUG EFFICACY; DUNALIELLA SALINA; GEL ELECTROPHORESIS; GREEN ALGA; HUMAN; HUMAN CELL; LUNG NON SMALL CELL CANCER; NITROBLUE TETRAZOLIUM TEST; PROTEIN EXPRESSION; SOLVENT EXTRACTION; STAIN; WESTERN BLOTTING;

EID: 45949088567     PISSN: 0258851X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Department of Health. Citation from http://www.doh.gov.tw/statistic/ index.htm 2006
2. Saba NF and Khuri FR: Chemoprevention strategies for patients with lung cancer in the context of screening. Clin Lung Cancer 7: 92-99, 2005.
3. Poppel GV and Goldbohm RA: Epidemiological evidences for h-carotene and cancer prevention. Am J Clin Nutr 62: 1393S-1402S, 1995.
4. Nesaretnam K, Radhakrishnan A and Selvaduray KR: Effect of palm oil carotene on breast cancer tumorigenicity in nude mice. Lipids 37: 557-560, 2002.
5. Lai BT: Effects of beta-carotene on lung cancer. Zhonghua Zhong Liu Za Zhi 15: 351-354, 1993.
6. Lin LM, Li BX, Xiao JB, Lin DH and Yang BF: Synergistic effect of all- trans-retinoic acid and arsenic trioxide on growth inhibition and apoptosis in human hepatoma, breast cancer, and lung cancer cells in vitro. World J Gastroenterol 11: 5633-5637, 2005.
7. Kim Y, Chongviriyaphan N, Liu C, Russell RM and Wang XD: Combined antioxidant ({beta}-carotene, {alpha}-tocopherol and ascorbic acid) supplementation increases the levels of lung retinoic acid and inhibits the activation of mitogen-activated protein kinase in the ferret lung cancer model. Carcinogenesis 27: 1410-1419, 2006.
8. Norsa A and Martino V: Somatostatin, retinoids, melatonin, vitamin D, bromocriptine, and cyclophosphamide in advanced non-small-cell lung cancer patients with low performance status. Cancer Biother Radiopharm 21: 68-73, 2006.
9. Chidambara Murthy KN, Vanitha A, Rajesba J, Mahadeva Swamy M, Sowmya PR and Ravishankar GA: In vivo antioxidant activity of carotenoids from DS - a green microalgae. Life Sci 76: 1381-1390, 2005.
10. Ben-Amotz A, Katz A and Avron M: Accumulation of β-carotene in halotolerant algae: purification and characterization of β-carotene rich globules from Dunaliella bardawil (chlorophyceae). J Phycol 18: 529-537, 1982.
11. Xue LX: Experimental study on extract of Dunaliella salina in preventing NSAR-induced cancer of the proventriculus in mice. Zhonghua Yu Fang Yi Xue Za Zhi 27: 350-353, 1993.
12. Nagasawa H, Fujii Y, Kageyama Y, Segawa T and Ben-Amotz A: Suppression by beta-carotene-rich algae Dunaliella bardawil of the progression, but not the development, of spontaneous mammary tumours in SHN virgin mice. Anticancer Res 11: 713-717, 1991.
13. Fujii Y, Ben-Amotz A, Sakamoto S and Nagasawa H: Effects of β-carotene rich algae Dunaliella bardawil on the dynamic changes of normal and neoplastic mammary cells and general metabolism in mice. Anticancer Res 13: 389-393, 1993.
14. Levin G, Yeshurun M and Mokady S: In vivo peroxidative effect of 9-cis-β-carotene compared with that of the all-trans isomer. Nutr Cancer 27: 293-297, 1997.
15. Raja R, Hemaiswarya S, Balasubramanyam D and Rengasamy R: Protective effect of Dunaliella salina (Volvocales, Chlorophyta) against experimentally induced fibrosarcoma on Wistar rats. Microbiol Res 162: 177-184, 2007.
16. Herrero M, Jaime L, Martín-Ivarez PJ, Cifuentes A and Ib ñez E: Optimization of the Extraction of Antioxidants from Dunaliella salina Microalga by Pressurized Liquids, J Agric Food Chem 54(15): 5597-5603, 2006.
17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radic Biol Med 26: 1231-1237, 1999.
18. Mossman T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63, 1983.
19. Ran ZH, Xu Q, Tong JL and Xiao SD: Apoptotic effect of epigallocatechin-3-gallate on the human gastric cancer cell line MKN45 via activation of the mitochondrial pathway. World J Gastroenterol 13: 4255-4259, 2007.
20. Mokady S, Abramovici A and Cogan U: The safety evaluation of Dunaliella bardawil as a potent food supplement. Food Chem Toxicol 4: 221-226, 1989.
21. Noda A, Ning SF, Venable OM, Pereira-Smith OM and Smith JR: Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Exp Cell Res 211: 90-98, 1994.
22. White E: Life, death, and the pursuit of apoptosis, Genes Dev 10: 1-15, 1996.
23. May P and May E: Twenty years of p53 research: structural and functional aspects of the p53 protein. Oncogene 18: 7621-7636, 1999.
24. Yoon JH and Gores GJ: Death receptor-mediated apoptosis and the liver. Hepatology 37: 400-410, 2002.
25. Muller M, Strand S, Hug H, Heinemann EM, Walczak H, Hofmann WJ, Stremmel W, Krammer PH and Galle PR: Drug-induced apoptosis in hepatoma cells is mediated by the CD95 (APO-1/Fas) receptor/ligand system and involves activation of wild-type p53. J Clin Invest 99: 403-413, 1997.
26. Gross A, McDonald JM, Stanely J and Korsmeyer J: Bcl-2 family members and the mitochondria in apoptosis. Genes Dev 13: 1899-1911, 1999.
27. Yang ZY, Perkins ND, Ohno T, Nabel EG and Nabel GJ: The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Nat Med 1: 1052-1056, 1995.
28. Bodzioch M, Dembinska-Kiec A, Hartwich J, Lapicka-Bodzioch K, Banas A, Polus A, Grzybowska J, Wybranska I, Dulinska J, Gil D, Laidler P, Placha W, Zawada M, Balana-Nowak A, Sacha T, Kiec-Wilk B, Skotnicki A, Moehle C, Langmann T and Schmitz G: The microarray expression analysis identifies BAX as a mediator of beta-carotene effects on apoptosis Nutr Cancer 51: 226-235, 2005.
29. Miyashita T and Reed JC: Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 80: 293-299, 1995.
30. Hosotani K, Yoshida M and Kitagawa M: Effects of administration of beta-carotene, ascorbic acid, persimmons, and pods on antioxidative ability in UV-irradiated ODS rats. Int J Vitam Nutr Res 75: 227-234, 2005.
31. Bohm F, Edge R, McGarvey DJ and Truscott TG: Beta-carotene with vitamins E and C offers synergistic cell protection against NOx. FEBS Lett 436: 387-389, 1998.
32. Bohm F: Cartenoids enhance vitamin E antioxidant efficiency. J Am Chem Soc 119: 621-622, 1997.
33. Weitberg AB and Corvese D: Effect of vitamin E and beta-carotene on DNA strand breakage induced by tobacco-specific nitrosamines and stimulated human phagocytes. J Exp Clin Cancer Res 16: 11-14, 1997.
34. Levin G and Mokady S: Antioxidant activity of 9-cis compared to all-trans β-carotene in vitro. Free Radic Biol Med 17: 77-82, 1994.
35. Bitterman N, Melamed Y and Ben-Amotz A: Beta-carotene and CNS oxygen toxicity in rats. J Appl Physiol 76: 1073-1076, 1994.
36. Hieber AD, King TJ, Morioka S, Fukushima LH, Franke AA and Bertram JS: Comparative effects of all-trans beta-carotene vs. 9-cis beta-carotene on carcinogen-induced neoplastic transformation and connexin 43 expression in murine 10T1/2 cells and on the differentiation of human keratinocytes. Nutr Cancer 37: 234-244, 2000.