Purification and characterization of a cathepsin L-like enzyme from the body wall of the sea cucumber Stichopus japonicus

Bioscience, Biotechnology and Biochemistry

Volumn 72, Issue 6, 2008, Pages 1430-1437

  Zhu, Bei Wei ^a   Zhao, Lu Lu ^a   Sun, Li Ming ^a   Li, Dong Mei ^a   Murata, Yoshiyuki ^b   Yu, Lei ^a   Zhang, Lei ^c  

^a DALIAN POLYTECHNIC UNIVERSITY   (China)

^b OKAYAMA UNIVERSITY   (Japan)

^c ZHEJIANG GONGSHANG UNIVERSITY   (China)

Author keywords

Cathepsin L; Characterization; Protease; Purification; Sea cucumber (Stichopus japonicus)

Indexed keywords

AMINO ACIDS; AMMONIUM COMPOUNDS; CHROMATOGRAPHIC ANALYSIS; COLLOIDS; DIFFUSERS (OPTICAL); FOOD ADDITIVES; GEL PERMEATION CHROMATOGRAPHY; GELATION; ION EXCHANGE; PURIFICATION; SULFATE MINERALS; WALLS (STRUCTURAL PARTITIONS);

AMMONIUM SULFATE PRECIPITATION; ARGININE (ARG); BODY WALLS; CATHEPSIN L; INTEGRAL METHODS; PHENYLALANINE (PHE); PURIFIED ENZYMES; SDS-PAGE; SEA CUCUMBER; SEPHADEX; SEPHAROSE; STICHOPUS JAPONICUS;

ENZYMES;

CATHEPSIN; CATHEPSIN L; CYSTEINE PROTEINASE; METAL; PROTEINASE INHIBITOR;

ANIMAL; ARTICLE; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; HISTOLOGY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEA CUCUMBER; TEMPERATURE;

ANIMALS; CATHEPSINS; CYSTEINE ENDOPEPTIDASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; MOLECULAR WEIGHT; PROTEASE INHIBITORS; SEA CUCUMBERS; SUBSTRATE SPECIFICITY; TEMPERATURE;

HOLOTHUROIDEA; STICHOPUS; STICHOPUS JAPONICUS;

EID: 45849144337     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.70741     Document Type: Article

References (36)

1. Kirschke, H., Barrett, A. J., and Rawlings, N. D., Lysosomal cysteine proteases. In "Protein Profile" Vol. 2, ed. Sheterline, P., Academic Press, London, pp. 1587-1643 (1995).
2. Kazunobu, T., Kiyoshi, O., Yukinori, N., Yukinori, N., Kenji, H., and Tadashi, I., Molecular characterization of cathepsin L from hepatopancreas of the carp Cyprinus carpio. Comp. Biochem. Physiol. B, 137, 107-114 (2004).
3. Kramer, G., Paul, A., Kreusch, A., Schülwer, S., Wiederanders, B., and Schilling, K., Optimized folding and activation of recombinant procathepsin L and S produced in Escherichia coli. Protein Expr. Purif., 54, 147-156 (2007).
4. Mason, R. W., and Barrett, A. J., Purification and properties of human liver cathepsin L. Biochem. Soc. Trans., 12, 1111 (1984).
5. Mason, R. W., Taylor, M. A. J., and Etherington, D. J., The purification and properties of cathepsin L from rabbit liver. J. Biochem., 217, 209-217 (1984).
6. Lee, J. J., Chen, H. C., and Jiang, S. T., Purification and characterization of proteinases identified as cathepsin L and L-like proteinase from mackerel (Scomber australasicus). Biosci. Biotechnol. Biochem., 57, 1470-1476 (1993).
7. Heu, M. S., Kim, H. R., Cho, D. M., Godber, J. S., and Pyeun, J. H., Purification and characterization of cathepsin L-like enzyme from the muscle of anchovy Engraulis japonica. Comp. Biochem. Physiol. B, 138, 523-529 (1997).
8. Ho, M. L., Chen, G. H., and Jiang, S. T., Effect of mackerel cathepsins L and L-like, and calpain on the degradation of mackerel surimi. Fish. Sci., 66, 558-568 (2000).
9. Kirschke, H., and Barrett, A. J., Chemistry of lysosomal proteases. In "Lysosomes: Their Role in Protein Breakdown," eds. Glaumann, H., and Ballard, F. J., Academic Press, London, pp. 193-238 (1987).
10. Visessanguan, W., Menino, A. R., Kim, S. M., and An, H., Cathepsin L: a predominant heat-activated proteinase in arrowtooth flounder muscle. Agric. Food Chem., 49, 2633-2640 (2001).
11. Dai, Z. Y., A review on autolysis in fish. J. Zhejiang Fish College, 9, 51-56 (1990).
12. Zhang, C. H., Deng, S. G., and Yang, L. M., Effect of ultraviolet ray and temperature on quick autolysis of shrimp. J. Zhanjiang Fish College, 14, 51-56 (1994).
13. Goll, D. E., Olsuka, Y., and Nagainis, P. A., Role of muscle proteinases in maintenance of muscle integrity and mass. J. Food Biochem., 7, 137 (1983).
14. Nishimura, K., Kawamura, Y., and Matob, T., Classification of proteases in Antarctic krill. Agric. Biol. Chem., 47, 2577-2583 (1983).
15. Qi, H., Dong, X. P., Cong, L. N., Gao, Y., Liu, L., Mikiro, T., and Zhu, B. W., Purification and characterization of a cysteine-like protease from the body wall of the sea cucumber Stichopus japonicus. Fish Physiol. Biochem., 33, 181-188 (2007).
16. Lowry, O. H., Rosebrough, N. J., and Farr, A. L., Protein measurement with the folin phenol reagent. Boil. Chem., 193, 265-275 (1951).
17. Barrett, A. J., and Kirschke, H., Cathepsin B, cathepsin H and cathepsin L. Meth. Enzymol., 80, 535-561 (1981).
18. Laemmli, U. K., Cleavage of structure proteins during the assembly of head of bacteriophage T4. Nature, 277, 680-685 (1970).
19. Lineweaver, H., and Burk, D., The determination of enzyme dissociation constants. J. Am. Chem. Sci., 56, 665-666 (1934).
20. Visessanguan, W., Benjakul, S., and An, H., Purification and characterization of cathepsin L in arrowtooth flounder (Atheresthes stomias) muscle. Comp. Biochem. Physiol. B, 134, 477-487 (2003).
21. Pike, R., Coetzer, T., and Dennison, C., Proteolytically active complexes of cathepsin L and a cysteine protease inhibitor: purification and demonstration of their formation in vitro. Arch. Biochem. Biophys., 294, 623-629 (1992).
22. An, H., Margo, Y., Thomas, S. A., and Michael, T. M., Isolation and activation of cathepsin L-inhibitor complex from Pacific whiting (Merluccius productus). J. Agric. Food Chem., 43, 327-330 (1995).
23. Yamashita, M., and Konagaya, S., An enzyme-inhibitor complex of cathepsin L in the white muscle of chum salmon (Oncorhynchus keta) in spawning migration. Comp. Biochem. Physiol. B, 103, 1005-1010 (1992).
24. Kawada, A., Hara, K., Kominami, E., Tezuka, T., Takahashi, M., and Takahara, H., Precursor of rat epidermal cathepsin L: purification and immunohistochemical localization. Dermatol. Sci., 23, 36-45 (2000).
25. Liu, H., Yin, L. J., Zhang, N., Li, S. H., and Ma, C. W., Purification and characterization of cathepsin L from the muscle of Silver Carp (Hypophthalmichthys molitrix). Agric. Food Chem., 54, 9584-9591 (2006).
26. Kargel, H. J., Dettmer, R., Etzold, G., Kirschke, H., Bohley, P., and Langner, J., Action of cathepsin L on the oxidized B-chain of bovine insulin. FEBS Lett., 114, 257-260 (1980).
27. Aranishi, F., Hara, K., Osatomi, K., and Ishihara, T., Purification and immunological properties of cathepsin B in carp Cyprinus carpio. Comp. Biochem. Physiol. B, 114, 371-376 (1996).
28. Aranishi, F., Hara, K., Osatomi, K., and Ishihara, T., Purification and characterization of cathepsin B from hepatopancreas of carp Cyprinus carpio. Comp. Biochem. Physiol. B, 117, 579-587 (1997).
29. Bando, Y., Kominami, E., and Katunuma, N., Purification and tissue distribution of rat cathepsin L. J. Biochem., 100, 35-42 (1986).
30. Baricos, W. H., Zhou, Y., Mason, R. W., and Barrett, A. J., Human kidney cathepsin B and L (characterization and potential role in degradation of glomerular basement membrane). J. Biochem., 252, 301-304 (1988).
31. Yamashita, M., and Konagaya, S., Participation of cathepsin L into extensive softening of the muscle of chum salmon caught during spawning migration. Bull. Jpn. Soc. Sci. Fish., 56, 1271-1277 (1990).
32. Coppes, Z., Martinez, G., and Hirschhorm, M., pH and temperature effects of the Km value of muscle lactate dehydrogenase isozyme LDH-A4 from fishes of the family sciaenidae (pereijormes). Comp. Biochem. Physiol. B, 103, 869-874 (1992).
33. Kariya, Y., Watabe, S., Hashimoto, K., and Yoshida, K., Occurrence of chondroitin sulfate E in glycosaminoglycan isolated from the body wall of sea cucumber Stichopus japonicus. J. Biol. Chem., 265, 5081-5085 (1990).
34. Kariya, Y., Watabe, S., Ochiai, Y., Hashimoto, K., and Murata, K., Glycosaminoglycan from the body wall of sea cucumber Stichopus japonicus. Comp. Biochem. Physiol., 95B, 387-392 (1990).
35. Kariya, Y., Watabe, S., Ochiai, Y., Murata, K., and Hashimoto, K., Glycosaminoglycan involved in the cation-induced change of body wall structure of sea cucumber Stichopus japonicus. Connect. Tissue Res., 25, 149-159 (1990).
36. Kariya, Y., Watabe, S., Kyogashima, M., Ishihara, M., and Ishii, T., The structure of branching fucans in the glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus. Carbohydr. Res., 297, 273-279 (1997).