Enzyme activity fingerprinting with substrate cocktails

Journal of the American Chemical Society

Volumn 126, Issue 36, 2004, Pages 11116-11117

  Goddard, Jean Philippe ^a   Reymond, Jean Louis ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ESTERASE; PHOSPHATASE; PHOSPHOTRANSFERASE; PROTEINASE; TRIACYLGLYCEROL LIPASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ACTIVITY FINGERPRINTING; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; PARAMETER; PROTEIN EXPRESSION; REACTION ANALYSIS; REPRODUCIBILITY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STEREOSPECIFICITY; TECHNIQUE;

ENZYMES; ESTERASES; LIPASE; PEPTIDE MAPPING;

EID: 4544222488     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0478330     Document Type: Article

References (40)

1. Steinmetz, L. M.; Davis, R. W. Nat. Rev. Genet. 2004, 5, 190-201.
2. (a) Gul, S.; Sreedharan, S. K.; Brocklehurst, K. In Enzyme Assays: A Practical Approach; Eisenthal, R., Danson, M., Eds.; Oxford University Press: New York, 2002.
3. (b) Reetz. M. T. Angew. Chem., Int. Ed. 2001, 40, 284-310.
4. (c) Wahler, D.; Reymond, J.-L. Curr. Opin. Chem. Biol, 2001, 5, 152-158.
5. (d) Beisson, F.; Tiss, A.; Rivière, C.; Verger, R. Eur. J. Lipid Sci. Technol. 2000, 133-153.
6. (e) Wahler, D.; Reymond, J.-L. Curr. Opin. Biotechnol. 2001, 12, 535-544.
7. (a) Schmid, A.; Dordick, J. S.; Hauer, B.; Kiener, A.; Wubbolts, M.; Witholt, B. Nature 2001, 409, 258-68.
8. (b) Marrs, B.; Delagrave, S.; Murphy, D. Curr. Opin. Microbiol. 1999, 2, 241-245.
9. Reymond, J.-L.; Wahler, D. ChemBioChem 2002, 3, 701-708.
10. Maly, D. J.; Huang, L.; Ellman, J. A. ChemBioChem 2002, 3, 16-37.
11. (a) Breddam, K.; Meldal, M. Eur. J. Biochem. 1992, 206, 103-107.
12. (b) Meldal, M.; Svendsen, I.; Breddam, K.; Auzanneau, F. I. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 3314-3318.
13. (c) St. Hilaire, P. M.; Willert, M.; Juliano, M. A.; Juliano, L.; Meldal, M. J. Comb. Chem. 1999, 1, 509-523.
14. (a) Thornberry, N. A.; Rano, T. A.; Peterson, E. P.; Rasper, D. M.; Timkey, T.; Garcia-Calvo, M.; Houtzager, V. M.; Nordstrom, P. A.; Roy, S.; Vaillancourt, J. P.; Chapman, K. T.; Nicholson, D. W. J. Biol. Chem. 1997, 272, 17907-17911.
15. (b) Harris, J. H.; Brackes, B. J.; Leonetti, F.; Mahrus, S.; Ellmann, J. A.; Craik, C. S. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 7754-7759.
16. (c) Harris, J. L.; Alper, P. B.; Li, J.; Reichsteiner, M.; Backes, B. J. Chem. Biol. 2001, 8, 1131-1141.
17. (d) Rano, T. A.; Timkey, T.; Peterson, E. P.; Rotonda, J.; Nicholson, D. W.; Becker, J. W.; Chapman, K. T.; Thornberry, N. A. Chem. Biol. 1997, 4, 149-155.
18. (e) Pinilla, C.; Appel, J. R.; Blanc, P.; Houghten, R. A. Biotechniques 1992, 13, 901-905.
19. (a) Liu, Y.; Patricelli, M. P.; Cravatt, B. F. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 14694-14699.
20. (b) Kidd, D.; Liu, Y.; Cravatt, B. F. Biochemistry 2001. 40, 4005-4015.
21. (c) Bogyo, M.; Verhelst, S.; Bellingard-Dubouchaud, V.; Toba, S.; Greenbaum, D. Chem. Biol. 2000, 7, 27-38.
22. (d) Greenbaum, D.; Medzihradszky, K. F.; Burlingame, A.; Bogyo, M. Chem. Biol. 2000, 7, 569-581.
23. (e) Nazif, T.; Bogyo, M. Proc. Natl Acad. Sci. U.S.A. 2001, 98, 2967-2972.
24. Sheppeck. J. E., II; Kar, H.; Gosink, L.; Wheatlev, J. B.; Gjerstad, E.; Loftus, S. M.; Zubiria, A. R.; Jane, J. W. Bioorg. Med. Chem. Lett. 2000, 10, 2639-2642.
25. (a) Salisbury, C. M.; Maly, D. J.; Ellman, J. A. J. Am. Chem. Soc. 2002, 124, 14868-14870.
26. (b) Zhu. Q.; Uttamchandani, M.; Li, D.; Lesaicherre, M. L.; Yao, S. Q. Org. Lett. 2003, 5, 1257-1260.
27. Toepert, F.; Knaute, T.; Guffler, S.; Pires, J.-R.; Matzdorf, T.; Oschkinat, H.; Schneider-Mergener, J. Angew. Chem., Int. Ed. 2003, 10, 1136-1140.
28. Liu, A. M. F.; Somers, N. A.; Kazlauskas, R. J.; Brush, T. S.; Zocher, F.; Enzelberger, M. M.; Bornscheuer, U. T.; Horsman, G. P.; Mezzetti. A.; Schmidt-Dannert. C.; Schmid, R. D. Tetrahedron: Asvmmetry 2001, 12, 545-556.
29. (a) Wahler, D.; Badalassi, F.; Crotti, P.; Reymond, J.-L. Angew. Chem., Int. Ed. 2001, 40, 4457-4460.
30. (b) Wahler, D.; Badalassi, F.; Crotti, P.; Reymond, J.-L. Chem. Eur. J. 2002, 8, 3211-3228.
31. (c) Wahler, D.; Boujard, O.; Lefèvre, F.; Reymond, J.-L. Tetrahedron 2004, 60, 703-710.
32. (d) Grognux, J.; Reymond, J.-L. ChemBioChem 2004, 5, 826-831.
33. Abecassis, V.; Pompon, D.; Truan, G. Nucleic Acids Res. 2000, 28, e88.
34. (a) Badalassi, F.; Wahler, D.; Klein, G.; Crotti, P.; Reymond. J.-L. Angew. Chem., Int. Ed. 2000, 39, 4067-4070.
35. (b) Gonzalez-Garcia, E. M.; Grognux, J.; Wahler, D.; Reymond, J.-L. Helv. Chim. Acta 2003, 86, 2458-2470.
36. (c) Nyfeler, E.; Grognux, J.; Wahler, D.; Reymond, J.-L. Helv. Chim. Acta 2003, 86, 2919-2927.
37. (a) http://www.unesco.org/idams.
38. (b) http://www.visualstats.org/.
39. Chatfield, C.; Collins, A. J. Introduction to Multivariate Analysis; Chapman and Hall, Ltd.: London. 1983.
40. The differentiating ability of our lipase cocktail is similar in its optically pure form (40 enantiomers in two HPLC analyses). An earlier report of lipase reactivity analysis by GC using a mixture of five different triglyceride substrates did not allow significant enzyme differentiation; see: Berger, M.; Schneider, M. P. Biotechnol. Lett. 1991, 13, 641-645.