Characterization of Aspergillus flavus strains from Brazilian Brazil nuts and cashew by RAPD and ribosomal DNA analysis

Letters in Applied Microbiology

Volumn 47, Issue 1, 2008, Pages 12-18

  Midorikawa, G E O ^a   Pinheiro, M R R ^a   Vidigal, B S ^a   Arruda, M C ^a   Costa, F F ^a   Pappas, G J ^a   Ribeiro, S G ^c   Freire, F ^b   Miller, R N G ^a  

^a UNIVERSIDADE CATÓLICA DE BRASÍLIA   (Brazil)

^b EMBRAPA AGROINDÚSTRIA TROPICAL   (Brazil)

^c EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA   (Brazil)

Author keywords

Aspergillus flavus; Detection; Internal amplification control; Mycotoxins; PCR; RAPD; rDNA ITS

Indexed keywords

ASPERGILLUS; DNA; POLYMERASE CHAIN REACTION;

AMPLIFICATION CONTROL; ASPERGILLUS FLAVUS; BRAZIL NUT; DETECTION; INTERNAL AMPLIFICATION; INTERNAL AMPLIFICATION CONTROL; POLYMORPHIC DNA; RANDOM AMPLIFIED POLYMORPHIC DNA; RDNA ITS; RIBOSOMAL DNA;

MYCOTOXINS;

AFLATOXIN; FUNGAL DNA; INTERNAL TRANSCRIBED SPACER 1; INTERNAL TRANSCRIBED SPACER 2; PRIMER DNA; RIBOSOME DNA;

BACTERIA; DNA; FUNGI;

CHROMATOGRAPHY; DETECTION METHOD; DNA; FUNGUS; LEGUME; POLYMERASE CHAIN REACTION; REPRODUCTIVE ISOLATION; TOXIN;

ARITHMETIC; ARTICLE; ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS OCCIDENTALE; BRAZIL; CHROMATOGRAPHY; CONTROLLED STUDY; DNA DETERMINATION; FALSE NEGATIVE RESULT; FUNGUS ISOLATION; GENE AMPLIFICATION; GENETIC VARIABILITY; HOST; NONHUMAN; NUCLEOTIDE SEQUENCE; NUT; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; SPECIES DIFFERENTIATION;

ANACARDIUM OCCIDENTALE; ASPERGILLUS FLAVUS; BERTHOLLETIA EXCELSA; FUNGI;

EID: 45249088308     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2008.02377.x     Document Type: Article

References (28)

1. Arrus, K., Blank, G., Abramson, D., Clear, R. Holley, R.A. (2005) Aflatoxin production by Aspergillus flavus in Brazil nuts. J Stored Prod Res 41, 513 527.
2. Castrillon, A.L. Purchio, A. (1988) Ocorrência de aflatoxinas em castanha do Para (Bertholletia excelsa Humb. & Bonpl.). Acta Amazonica 18, 49 56.
3. Chen, R.S., Chen, R.S., Tsay, J.G. Huang, Y.F. (2002) Polymerase chain reaction-mediated characterization of molds belonging to the Aspergillus flavus group and detection of Aspergillus parasiticus in peanut kernels by a multiplex polymerase chain reaction. J Food Protect 65, 840 844.
4. Codex Alimentarius 2003) Hazard analysis and critical control point (HACCP) system and guidelines for its application. ANNEX to Recommended International Code of Practice/General Principles of Food Hygiene. CAC/RCP 1-1969, Rev 4. Rome : FAO/WHO Codex Alimentarius Commission.
5. Freire, F.C.O., Kozakiewicz, Z. Paterson, R.R.M. (1999) Mycoflora and mycotoxins of Brazilian cashew kernels. Mycopathologia 145, 95 103.
6. Freire, F.C.O., Kozakiewicz, Z. Paterson, R.R.M. (2000) Mycoflora and mycotoxins in Brazilian black pepper, white pepper and Brazil nuts. Mycopathologia 149, 13 19.
7. Geisen, R. (1998) PCR methods for the detection of mycotoxin-producing fungi. In Applications of PCR in Mycology ed. Bridge, P.D., Arora, D.K., Reddy, C.A. Elander, R.P. pp. 243 266. Wallingford, Oxon : CAB International.
8. Griffin, G.J., Smith, E.P. Robinson, T.J. (2001) Population patterns of Aspergillus flavus group and A. niger group in field soil. Soil Biol Biochem 33, 253 257.
9. Khan, Z.K., Chowdhary, L., Gyanchandani, A., Goswami, M., Farooqui, N. Ranade, S.A. (1998) Aspergillus fumigatus strains recovered from immunocompromised patients (ICP): subtyping of strains by RAPD analysis. Biochem Mol Biol Int 46, 537 548.
10. Kovach, W.L 1999) MVSP - A Multivariate Statistical Package for Windows, ver 3.1. Pentraeth, Wales, UK : Kovach Computing Services.
11. Kumeda, Y. Asao, T. (1996) Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section flavi. Appl Environ Microb 62, 2947 2952.
12. Lin, M.T. Dianese, J.G. (1976) A coconut agar medium for rapid detection of aflatoxin production by Aspergillus species. Phytopathology 66, 1466 1469.
13. Lourenço, A., Durigon, E. L., Zanotto, P., Madeira, J. E. G. C., de Almeida, A. P. Correa, B. (2007) Genetic diversity of environmental Aspergillus flavus strains in the state of São Paulo, Brazil by random amplified polymorphic DNA. Mem Inst Oswaldo Cruz 102, 687 692.
14. Niessen, L., Schmidt, H., Muhlencoert, E., Farber, P., Karolewiez, A. Geisen, R. (2005) Advances in the molecular diagnosis of ochratoxin A-producing fungi. Food Addit Contam 22, 324 334.
15. Paterson, R.R.M. (2006) Identification and quantification of mycotoxigenic fungi by PCR. Process Biochem 41, 1467 1474.
16. Paterson, R.R.M. (2007) Internal amplification controls have not been employed in diagnostic fungal PCR hence potential false negative results. J Appl Microbiol 102, 1 10.
17. Pitt, J.L. Hocking, A.D 1991) Significance of fungi in stored products. In Fungi and Mycotoxins in Stored Products, Vol 36, ed. Champ, B.R., Highley, E., Hocking, A.D. Pitt, J.L. pp. 16 21. Canberra : ACIAR Proc.
18. Pitt, J.L. Hocking, A.D 1997) Fungi and Food Spoilage. London : Blackie Academic & Professional.
19. Pitt, J.L., Rocking, A.D., Bhudhasamai, K., Miscamble, B.F., Wheeler, K.A. Tamboon, E.K.P. (1993) The normal mycoflora of commodities from Thailand. 1. Nuts and oilseeds. Int J Food Microbiol 20, 211 226.
20. Raeder, U. Broda, P. (1985) Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1, 17 20.
21. Rozen, S. Skaletsky, H.J. (2000) Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols: Methods in Molecular Biology ed. Krawetz, S. Misener, S. pp. 365 386 Totowa, NJ : Humana Press.
22. Salay, E 2003) Case Study: Reducing Mycotoxins in Brazilian Crops. In Food Safety in Food Security and Food Trade ed. Unnevehr, L.J. Brief 15 of 17. IFPRI 2020 Vision Focus No. 10. Washington, DC : International Food Policy Research Institute.
23. Scherm, B., Palomba, M., Serra, D., Marcello, A. Migheli, Q. (2005) Detection of transcripts of the aflatoxin genes af lD, aflO, and aflP by reverse transcription-polymerase chain reaction allows differentiation of aflatoxin-producing and non-producing isolates of Aspergillus flavus and Aspergillus parasiticus. Int J Food Microbiol 98, 201 210.
24. Shapiro, R., Paster, N., Eyal., O., Menasherov, M., Mett, A. Salomon, R. (1996) Detection of aflatoxigenic molds in grains by PCR. Appl Environ Microb 62, 3270 3273.
25. Singh, K., Frisvad, J.C., Thrane, U. Mathur, S.B 1991) An Illustrated Manual on Identification of Some Seed-Borne Aspergilli, Fusaria, Penicillia and their Mycotoxins. Jordbrugsforlaget, Frederiksberg, Denmark : Danish Government Institute of Seed Pathology for Developing Countries, Denmark, and Department of Biotechnology, The Technical University of Denmark.
26. Sugita, C., Makimura, K., Uchida, K., Yamaguchi, H. Nagai, A. (2004) PCR identification system for the genus Aspergillus and three major pathogenic species: Aspergillus fumigatus, Aspergillus flavus and Aspergillus niger. Med Mycol 42, 433 437.
27. Thompson, J.D., Higgins, D.G. Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673 4680.
28. White, T.J., Bruns, T., Lee, S. Taylor, J.W. (1990) Amplification and direct sequencing of fungal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications ed. Innis, M.A., Gelgard, D.H., Sninsky, J.J. White, T.J. pp. 315 322 New York : Academic Press.