Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus Section Flavi

Applied and Environmental Microbiology

Volumn 62, Issue 8, 1996, Pages 2947-2952

  Kumeda, Yuko ^a   Asao, Tsutomu ^a  

^a OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ETHIDIUM BROMIDE; FORMAMIDE; RIBOSOME DNA;

ARTICLE; ASPERGILLUS; GENE AMPLIFICATION; ISOTOPE LABELING; POLYMERASE CHAIN REACTION; PROTEIN DENATURATION; SINGLE STRAND CONFORMATION POLYMORPHISM; SPECIES DIFFERENCE; SPECIES DIFFERENTIATION;

EID: 0029760862     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.8.2947-2952.1996     Document Type: Article

References (28)

1. Ainsworth, P. J., L. C. Surh, and M. B. Coulter-Mackie. 1991. Diagnostic single strand conformational polymorphism (SSCP): a simplified non-radioisotopic method as applied to a Tay-Sachs B1 variant. Nucleic Acids Res. 19:405-406.
2. Bainbridge, B. W., C. L. Spreadbury, F. G. Scalise, and J. Cohen. 1990. Improved methods for the preparation of high molecular weight DNA from large and small scale cultures of filamentous fungi. FEMS Microbiol. Lett. 66:113-118.
3. Bernier, L., R. C. Hamelin, and G. B. Ouellette. 1994. Comparison of ribosomal DNA length and restriction site polymorphisms in Gremmeniella and Ascocalyx isolates. Appl. Environ. Microbiol. 60:1279-1286.
4. Chang, P.-K., D. Bhatnagar, T. E. Cleveland, and J. W. Bennett. 1995. Sequence variability in homologs of the aflatoxin pathway gene afIR distinguishes species in Aspergillus Section Flavi. Appl. Environ. Microbiol. 61:40-43.
5. Cotty, P. J., P. Bayman, D. S. Egel, and K. S. Elias. 1994. Agriculture, aflatoxins and Aspergillus, p. 1-27. In K. A. Powell, A. Renwick, and J. F. Peberdy (ed.). The genus Aspergillus: from taxonomy and genetics to industrial applications. Plenum Press, New York.
6. Egel, D. S., P. J. Cotty, and K. S. Elias. 1994. Relationships among isolates of Aspergillus sect. flavi that vary in aflatoxin production. Phytopathology 84:906-912.
7. Glavac, D., and M. Dean. 1993. Optimization of the single-strand conformation polymorphism (SSCP) technique for detection of point mutations. Hum. Mutat. 2:404-414.
8. Hibbett, D. S. 1992. Ribosomal RNA and fungal systematics. Trans. Mycol. Soc. Jpn 33:533-556.
9. Klich, M. A., and J. I. Pitt. 1988. Differentiation of Aspergillus flavus from A. parasiticus and other closely related species. Trans. Br. Mycol. Soc. 91:99-108.
10. Kurtzman, C. P., B. W. Horn, and C. W. Hesseltine. 1987. Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie Leeuwenhoek 53:147-158.
11. Kurtzman, C. P., M. J. Smiley, C. J. Robnett, and D. T. Wicklow. 1986. DNA relatedness among wild and domesticated species in the Aspergillus flavus group. Mycologia 78:955-959.
12. Li, K.-N., T. L. German, and D. I. Rouse. 1992. A 30 base pair region in the mitochondrial small subunit rRNA gene as a specific DNA probe for Verticillium dahliae. Phytopathology 82:1145.
13. McAlpin, C. E., and B. Mannarelli. 1995. Construction and characterization of a DNA probe for distinguishing strains of Aspergillus flavus. Appl. Environ. Microbiol. 61:1068-1072.
14. Messner, R., H. Prillinger, M. Ibl, and G. Himmler. 1995. Sequences of ribosomal genes and internal transcribed spacers move three plant parasitic fungi, Eremothecium ashbyi, Ashbya gossypii, and Nematospora coryli, toward Saccharomyces cerevisiae. J. Gen. Appl. Microbiol. 41:31-42.
15. Molina, F. I., L. M. Geletka, S.-C. Jong, and Y. Zhang. 1994. Use of a nested primer pair as control for PCR amplification of ribosomal DNA internal transcribed spacers in fungi. BioTechniques 16:998-1001.
16. Moody, S. F., and B. M. Tyler. 1990. Restriction enzyme analysis of mitochondrial DNA of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius. Appl. Environ. Microbiol. 56:2441-2452.
17. Moody, S. F., and B. M. Tyler. 1990. Use of nuclear DNA restriction fragment length polymorphisms to analyze the diversity of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius. Appl. Environ. Microbiol. 56:2453-2461.
18. Orita, M., H. Iwahana, H. Kanazawa, K. Hayashi, and T. Sekiya. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86:2766-2770.
19. Pinder, J. C., D. Z. Staynov, and W. B. Gratzer. 1974. Properties of RNA in formamide. Biochemistry 13:5367-5373.
20. Pinder, J. C., D. Z. Staynov, and W. B. Gratzer. 1974. Electrophoresis of RNA in formamide. Biochemistry 13:5373-5378.
21. Ravnik-Glavac, M., D. Glavac, and M. Dean. 1994, Sensitivity of single-strand conformation polymorphism and heteroduplex method for mutation detection in the cystic fibrosis gene. Hum. Mol. Genet. 3:801-807.
22. Rossetti, S., S. Corra, M. O. Biasi, A. E. Turco, and P. F. Pignatti. 1995. Comparison of heteroduplex and single-strand conformation analyses, followed by ethidium fluorescence visualization, for the detection of mutations in four human genes. Mol. Cell. Probes 9:195-200.
23. Saito, K. 1943. On the scientific name of aspergilli isolated in Japan. Nippon Jozo Kyokai Zasshi 38:412-414.
24. Soto, D., and S. Sukumar. 1992. Improved detection of mutations in the p53 gene in human tumors as single-stranded conformation polymorphs and double-stranded heteroduplex DNA. PCR Methods Applic. 2:96-98.
25. Sun, Y., D. Z. Skinner, G. H. Liang, and S. H. Hulbert. 1994. Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA. Theor. Appl. Genet. 89:26-32.
26. White, T. J., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, p. 315-322. In M. A. Innis, D. H. Gelfand, J. Sninsky, and T. J. White (ed.), PCR protocols, a guide to methods and applications. Academic Press, Inc., San Diego, Calif.
27. Woloshuk, C. P., K. R. Foutz, J. F. Brewer, D. Bhatnagar, T. E. Cleveland, and G. A. Payne. 1994. Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis. Appl. Environ. Microbiol. 60:2408-2414.
28. Yuan, G.-F., C.-S. Liu, and C.-C. Chen. 1995. Differentiation of Aspergillus parasiticus from Aspergillus sojae by random amplification of polymorphic DNA. Appl. Environ. Microbiol. 61:2384-2387.