Improvement in the suspension-culture production of recombinant adeno-associated virus-LacZ in HEK-293 cells using polyethyleneimine-DNA complexes in combination with hypothermic treatment

Biotechnology and Applied Biochemistry

Volumn 50, Issue 2, 2008, Pages 121-132

  Feng, Lei ^a   Guo, Meijin ^a   Zhang, Shuxiang ^a   Chu, Ju ^a   Zhuang, Yingping ^a   Zhang, Siliang ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Human embryonic kidney 293 cells (HEK 293 cells); Hypothermia; Polyethylenimine (PEI); Recombinant adeno associated virus (rAAV); Transfection complexes; Transient transfection

Indexed keywords

CELLS; DNA; GENES; GENETIC ENGINEERING; HYPOTHERMIA; MICROFLUIDICS; NUCLEIC ACIDS; ORGANIC ACIDS; RHENIUM; SUSPENSIONS (COMPONENTS); VIRUSES;

ADENO-ASSOCIATED VIRUS (AAV); CELL CYCLE ARREST; CELL DENSITIES; DELIVERY VECTOR; DNA COMPLEXES; GALACTOSIDASE; GENE THERAPIES; HEK 293 CELLS; HUMAN EMBRYONIC KIDNEY (HEK); HYPOTHERMIC TREATMENT; KEY FACTORS; POLYETHYLENE IMINE (PEI); REPORTER GENES; SUSPENSION-CULTURED; TRANSFECTION EFFICIENCY;

CELL CULTURE;

BETA GALACTOSIDASE; POLYETHYLENEIMINE; VIRUS VECTOR; DNA; DRUG CARRIER; RECOMBINANT PROTEIN;

ADENOVIRUS; ARTICLE; CELL CULTURE; CELL CYCLE ARREST; CELL STRAIN HEK293; CONTROLLED STUDY; HUMAN; HUMAN CELL; REPORTER GENE; CELL LINE; CHEMISTRY; CULTURE TECHNIQUE; GENETIC TRANSFECTION; GENETICS; HEAT; KIDNEY; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN ENGINEERING;

ADENO-ASSOCIATED VIRUS;

ADENOVIRIDAE; BETA-GALACTOSIDASE; CELL CULTURE TECHNIQUES; CELL LINE; DNA; DRUG CARRIERS; HEAT; HUMANS; KIDNEY; POLYETHYLENEIMINE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 44849094187     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070081     Document Type: Article

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