Uricase production by a recombinant Hansenula polymorpha strain harboring Candida utilis uricase gene

Applied Microbiology and Biotechnology

Volumn 79, Issue 4, 2008, Pages 545-554

  Chen, Zhiyu ^a,b   Wang, Zhaoyue ^a   He, Xiuping ^a   Guo, Xuena ^a   Li, Weiwei ^a   Zhang, Borun ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Candida utilis; Hansenula polymorpha; pH control; Recombinant uricase

Indexed keywords

CELL CULTURE; FERMENTATION; METHANOL; PEPTIDES; PH EFFECTS; YEAST;

CANDIDA UTILIS; HANSENULA POLYMORPHA; MEDICAL ENZYMES; RECOMBINANT URICASE;

GENE EXPRESSION;

FUNGAL ENZYME; URATE OXIDASE;

CELL ORGANELLE; ENZYME ACTIVITY; GENE; METHANOL; PEPTIDE; PH; YEAST;

ACIDITY; ANALYTICAL PARAMETERS; ARTICLE; CANDIDA UTILIS; CELL DENSITY; ENZYME SYNTHESIS; FED BATCH FERMENTATION; FUNGAL GENE; FUNGAL STRAIN; FUNGUS CULTURE; GENE EXPRESSION SYSTEM; HANSENULA POLYMORPHA; HETEROLOGOUS EXPRESSION; NONHUMAN;

BIOMASS; CANDIDA; CELL CULTURE TECHNIQUES; CULTURE MEDIA; FERMENTATION; FUNGAL PROTEINS; GENE EXPRESSION; GENETIC ENGINEERING; MOLECULAR WEIGHT; PICHIA; RECOMBINANT PROTEINS; TIME FACTORS; URATE OXIDASE;

PICHIA ANGUSTA; PICHIA JADINII; SACCHAROMYCES CEREVISIAE;

EID: 44649168810     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1472-8     Document Type: Article

References (37)

1. Adams A, Gottschling DE, Kaiser CA, Stearns T (1997) Methods in yeast genetics. A Cold Spring Harbor laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
2. Bonnete F, Vivares D, Robert CH, Colloch N (2001) Interactions in solution and crystallization of Aspergillus flavus urate oxidase. J Cryst Growth 232:330-339
3. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 13:329-332
4. Fitzpatrick DA, McGeeney KF (1975) Antigenic independence of some microbial urate oxidases. Infect Immun 12:1237-1241
5. Gellissen G, Piontek M, Dahlems U, Jenzelewski V, Gavagan JE, DiCosimo R, Anton DL, Janowicz ZA (1996) Recombinant Hansenula polymorpha as a biocatalyst: coexpression of the spinach glycolate oxidase (GO) and the Saccharomyces cerevisiae catalase T (CTT1) gene. Appl Microbiol Biotechnol 46:46-54
6. Gellissen G (2000) Heterologous protein production in methylotrophic yeasts. Appl Microbiol Biotechnol 54:741-750
7. Gellissen G, Kunze G, Gaillardin C, Cregg JM, Berardi E, Veenhuis M, van der Klei I (2005) New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica-a comparison. FEMS Yeast Res 5:1079-1096
8. Gietl C, Faber KN, van der Klei IJ, Veenhuis M (1994) Mutational analysis of the N-terminal topogenic signal of watermelon glyoxysomal malate dehydrogenase using the heterologous host Hansenula polymorpha. Proc Natl Acad Sci USA 91:3151-3155
9. Inan M, Meagher MM (2001) Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris. J Biosci Bioeng 92:585-589
10. Jahic M, Gustavsson M, Jansen AK, Martinelle M, Enfors SO (2003) Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J Biotechnol 102:45-53
11. Kang HA, Kang W, Hong WK, Kim MW, Kim JY, Sohn J, Choi ES, Choe KB, Rhee SK (2001) Development of expression systems for the production of recombinant human serum albumin using the MOX promoter in Hansenula polymorpha DL-1. Biotechnol Bioeng 76:175-85
12. Klabunde J, Kleebank S, Piontek M, Hollenberg CP, Hellwig S, Degelmann A (2007) Increase of calnexin gene dosage boosts the secretion of heterologous proteins by Hansenula polymorpha. FEMS Yeast Res 7:1168-1180
13. Klose S, Stoltz M, Munz E, Portenhauser R (1978) Determination of uric acid on continuous-flow (AutoAnalyzer II and SMA) systems with a uricase/phenol/4-aminophenazone color test. Clin Chem 24:250-255
14. Koyama Y, Ichikawa T, Nakano E (1996) Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding the Candida utilis urate oxidase (uricase). J Biochem (Tokyo) 120:969-973
15. Laborde C, Chemardin P, Bigey F, Combarnous Y, Moulin G, Boze H (2004) Overexpression of ovine leptin in Pichia pastoris: physiological yeast response to leptin production and characterization of the recombinant hormone. Yeast 21:249-263
16. Ledeboer AM, Edens L, Maat J, Visser C, Bos JW, Verrips CT, Janowicz Z, Eckart M, Roggenkamp R, Hollenberg CP (1985) Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha. Nucleic Acids Res 13:3063-3082
17. Leplatois P, Le Douarin B, Loison G (1992) High-level production of a peroxisomal enzyme: Aspergillus flavus uricase accumulates intracellularly and is active in Saccharomyces cerevisiae. Gene 122:139-145
18. Li J, Chen Z, Hou L, Fan H, Weng S, Xu C, Ren J, Li B, Chen W (2006) High-level expression, purification, and characterization of non-tagged Aspergillus flavus urate oxidase in Escherichia coli. Protein Expr Purif 49:55-59
19. Linder S, Schliwa M, Kube-Granderath E (1996) Direct PCR screening of Pichia pastoris clones. Biotechniques 20:980-982
20. Liu J, Li G, Liu H, Zhou X (1994) Purification and properties of uricase from Candida sp. and its application in uric acid analysis in serum. Appl Biochem Biotechnol 47:57-63
21. Lotfy WA (2008) Production of a thermostable uricase by a novel Bacillus thermocatenulatus strain. Bioresour Technol 99:699-702
22. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22:249-270
23. Mayson BE, Kilburn DG, Zamost BL, Raymond CK, Lesnicki GJ (2003) Effects of methanol concentration on expression levels of recombinant protein in fed-batch cultures of Pichia methanolica. Biotechnol Bioeng 81:291-298
24. Moon H, Kim H, Rhee SK, Choi ES, Kim IH, Hong SI (2002) Optimal strategy of pH control in the production of recombinant human epidermal growth factor by Hansenula polymorpha. Process Biochem 38:487-495
25. Muller F, Tieke A, Waschk D, Muhle C, Muller F, Seigelchifer M, Pesce A, Jenzelewski V, Gellissen G (2002) Production of IFNa-2a in Hansenula polymorpha. Process Biochem 38:15-25
26. Nelson DL, Cox MM (2004) Lehninger's principles of biochemistry, 4th edn. Freeman, New York, pp 866-876
27. Oda M, Satta Y, Takenaka O, Takahata N (2002) Loss of urate oxidase activity in hominoids and its evolutionary implications. Mol Biol Evol 19:640-653
28. Oldfield V, Perry CM (2006) Rasburicase: a review of its use in the management of anticancer therapy-induced hyperuricaemia. Drugs 66:529-545
29. Sambrook J, Russell DW (2001) Molecular cloning, a laboratory manual, 3rd edn. Cold Spring Harbor, New York
30. Shi X, Karkut T, Chamankhah M, Alting-Mees M, Hemmingsen SM, Hegedus D (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expr Purif 28:321-330
31. Sinha J, Plantz BA, Zhang W, Gouthro M, Schlegel V, Liu C-P, Meagher MM (2003) Improved production of recombinant ovine interferon-t by Mutt strain of Pichia pastoris using an optimized methanol feed profile. Biotechnol Prog 19:794-802
32. Suzuki H, Verma DP (1991) Soybean nodule-specific uricase (Nodulin-35) is expressed and assembled into a functional tetrameric holoenzyme in Escherichia coli. Plant Physiol 95:384-389
33. Suzuki K, Sakasegawa S, Misaki H, Sugiyama M (2004) Molecular cloning and expression of uricase gene from Arthrobacter globiformis in Escherichia coli and characterization of the gene product. J Biosci Bioeng 98:153-158
34. van der Heide M, Hollenberg CP, van der Klei IJ, Veenhuis M (2002) Overproduction of BiP negatively affects the secretion of Aspergillus niger glucose oxidase by the yeast Hansenula polymorpha. Appl Microbiol Biotechnol 58:487-494
35. Vogt B (2005) Urate oxidase (rasburicase) for treatment of severe tophaceous gout. Nephrol Dial Transplant 20:431-433
36. Wu XW, Lee CC, Muzny DM, Caskey CT (1989) Urate oxidase: primary structure and evolutionary implications. Proc Natl Acad Sci USA 86:9412-9416
37. Yazdi MT, Zarrini G, Mohit E, Faramarzi MA, Setayesh N, Sedighi N, Mohseni FA (2006) Mucor hiemalis: a new source for uricase production. World J Microbiol Biotechnol 22:325-330