Optimal strategy of pH control in the production of recombinant human epidermal growth factor by Hansenula polymorpha

Process Biochemistry

Volumn 38, Issue 4, 2002, Pages 487-495

  Moon, H ^a   Kim, H ^a   Rhee, S K ^b   Choi, E S ^b   Kim, I H ^a   Hong, S I ^a  

^a Korea University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology   (United States)

Author keywords

Culture pH; Degradation; Hansenula polymorpha; Human epidermal growth factor; Modeling; pH control

Indexed keywords

BACTERIA (MICROORGANISMS); PICHIA; PICHIA ANGUSTA;

EID: 0037010798     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0032-9592(02)00172-3     Document Type: Article

References (26)

1. Gellissen G, Janowicz ZA, Merckelbach A, Piontek M, Keup P, Weydemann U, et al. Heterologous gene expression in Hansenula polymorpha: Efficient secretion of glucoamylase. Bio/Technology 1991;9:291-5.
2. Gellissen G, Hollenberg CP, Janowicz ZA. Gene Expression in Methylotrophic Yeasts. Gene Expression in Recombinant Microorganisms. New York: Marcel Dekker, 1994:195-239.
3. Carpenter G, Cohen S. Epidermal growth factor. Ann Rev Biochem 1979;48:193-216.
4. Brierley RA, Siegel RS, Bussineau CM, Craig WS, Holtz GC, Davis GR, et al. Mixed Feed Recombinant Yeast Fermentation. US Patent, WO 9003431, 1990.
5. Coppella ST, Dhurjati P. α-Factor directed expression of the human epidermal growth factor in Saccharomyces cerevisiae. Biotechnol Bioeng 1989;33:976-83.
6. Ebisu S, Takagi H, Kodowaki K, Yamagata H, Udaka S. The efficient production of human epidermal growth factor by Bacillus brevis. Ann NY Acad Sci 1996;782:115-22.
7. Hamsa PV, Kachroo P, Chattoo BB. Production and secretion of biologically active human epidermal growth factor in Yarrowia lipolytica. Curr Genet 1998;33:231-7.
8. Sivakesava S, Xu ZN, Chen YH, Hachett J, Huang RC, Lam E, et al. Production of excreted human epidermal growth ractor (hEGF) by an efficient recombinant Echerichia coli system. Proc Biochem 1999;34:893-900.
9. Choi ES, Rhee SK, Sohn JH, Kang HA, Bae JH. Hansenula Polymorpha Gene and Strain Lacking of the Same. Korean Patent Pending, File No. 99-03095, 1999.
10. Rhee SK, Choi ES, Kim CH, Sohn JH, Kang HA, Kim HY. Autonomously Replicating Sequences, GADPH Gene and Promoter Derived from Hansenula Polymorpha, Expression Vectors Containing Same and Method for the Selection of Transformants. US Patent 5,935,789, 1999.
11. Chiruvolu V, Eskridge K, Cregg J, Meagher M. Effects of glycerol concentration and pH on growth of recombinant Pichia pastoris Yeast. Appl Biochem Biotechnol 1999;75:163-73.
12. Roubin MR, Cailas MD, Shen SH, Groleau D. Influence of pH, nitrogen and phosphorous sources on the production of hepatitis B virus pre-S2 antigen by Hansenula polymorpha. Appl Microbiol Biotechnol 1992;38:158-64.
13. Bae CS, Yang DS, Lee J, Park YH. Improved process for production of recombinant yeast-derived monomeric human G-CSF. Appl Microbiol Biotechnol 1999;52:338-44.
14. Clare JJ, Romanos MA, Rayment FB, Rowedder JE, Smith MA, Payne MM, et al. Production of mouse epidermal growth factor in yeast: High-level secreting using Pichia pastoris strains containing multiple gene copies. Gene 1991;105:205-12.
15. Werten MWT, Bosch TJ, Wind RD, Mooibroek H, Wolf FA. High yield secretion of recombinant of recombinant gelatins by Pichia pastoris. Yeast 1999;15:1087-96.
16. Inan M, Chiruvolu V, Eskridge KM, Vlasuk GP, Dickerson K, Brown S, et al. Optimization of temperature - Glycerol - pH conditions for a fed-batch fermentation process for recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (AcAP-5) production by Pichia pastoris. Enzyme Microb Technol 1999;24:438-45.
17. Song GY, Chung BH. Use of high culture pH for the decreased proteolysis of human parathyroid hormone secreted by Saccharomyces cerevisiae. Biotechnol Lett 1999;21:57-60.
18. Kim CH, Sohn JH, Choi ES, Rhee SK. Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha. Biotechnol Lett 1996;18:417-22.
19. Chung BH, Park KS. Simple approach to reducing proteolysis during secretary production of human parathyroid hormone in Saccharomyces cerevisiae. Biotechnol Bioeng 1998;22:245-9.
20. Kang HA, Kim SJ, Choi ES, Rhee SK, Chung BH. Efficient production of intact human parathyroid hormone in a Saccharomyces cerevisiae mutant deficient in yeast aspartic protease 3 (YAP3). Appl Microbiol Biotechnol 1998;50:187-92.
21. Siegel RS, Brierley RA. Use of a cell recycle reactor to increase production of a proteolysis-susceptible peptide secreted from recombinant Saccharomyces cerevisiae. Bio/Technology 1990;8:639-43.
22. Park S, Ramirez WF. Optimal production of secreted protein in fed-batch reactors. AIChE 1988;34:1550-8.
23. Voller A, Bidwell DE, Bartlet A. The Enzyme Linked Immunosorbent Assay (ELISA), a Guide with Abstracts of Microplate Application. Dynatech Laboratories, INC, 1979:10-43.
24. Brierley RA, Davis GR, Holtz RG. Production of Insulin-like Growth Factor-1 in Methylotrophic Yeast Cells. US Patent 5,324,639, 1994.
25. Siegel RS, Buckholtz RG, Thill GP, Wondrack LM. Production of Epidermal Growth Factor in Methylotrophic Yeast Cells. US Patent WO 90/10697 1990.
26. Yamane T, Shimizu S. Fed-batch techniques in microbial process. Adv Biochem Eng 1984;30:147-94.