Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9°N-7 and mutations affecting 3′-5′ exonuclease activity

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 11, 1996, Pages 5281-5285

  Southworth, M W ^a   Kong, H ^a   Kucera, R B ^a   Ware, J ^a   Jannasch, H W ^b   Perler, F B ^a  

^a NEW ENGLAND BIOLABS   (United States)

^b WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

Author keywords

Intein; Mutation; Protein splicing; Purification

Indexed keywords

ARCHAEA; ESCHERICHIA COLI; THERMOCOCCUS LITORALIS; THERMOCOCCUS SP.; BACTERIA (MICROORGANISMS);

DNA POLYMERASE;

AMINO ACID SUBSTITUTION; ARCHAEBACTERIUM; ARTICLE; BACTERIUM MUTANT; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE MUTATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ARCHAEA; BASE SEQUENCE; CLONING, MOLECULAR; DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; ESCHERICHIA COLI; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; RECOMBINANT PROTEINS;

EID: 0030014622     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.11.5281     Document Type: Article

References (24)

1. Perler, F. B., Comb, D. G., Jack, W. E., Moran, L. S., Qiang, B., Kucera, R. B., Benner, J., Slatko, B. E., Nwankwo, D. O., Hempstead, S. K., Carlow, C. K. S. & Jannasch, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5577-5581.
2. Uemori, T., Ishino, Y., Toh, H., Asada, K. & Kato, I. (1993) Nucleic Acids Res. 21, 259-265.
3. Braithwaite, D. K. & Ito, J. (1993) Nucleic Acids Res. 21, 787-802.
4. Perler, F. B., Kumar, S. & Kong, H. (1996) in Enzymes and Proteins from Hyperthermophilic Microorganisms: Advances in Protein Chemistry, ed. Adams, M. W. W. (Academic, New York), Vol. 48, in press.
5. Perler, F. B., Davis, E. O., Dean, G. E., Gimble, F. S., Jack, W. E., Neff, N., Noren, C. J., Thorner, J. & Belfort, M. (1994) Nucleic Acids Res. 22, 1125-1127.
6. Clyman, J. (1995) Am. Soc. Microbiol. News 61, 344-347.
7. Cooper, A. A. & Stevens, T. H. Trends Biochem. Sci. 20, 351-357.
8. Blanco, L., Bernad, A., Blasco, M. A. & Salas, M. (1991) Gene 100, 27-38.
9. Morrison, A., Bell, J. B., Kunkel, T. A. & Sugino, A. (1991) Proc. Natl. Acad. Sci. USA 88, 9473-9477.
10. Derbyshire, V., Pinsonneault, J. K. & Joyce, C. M. (1995) Methods Enzymol. 262, 363-388.
11. Jannasch, H. W. (1990) in Bioprocessing and Biotreatment of Coal, ed. Wise, D. L. (Dekker, New York), pp. 417-428.
12. Jannasch, H. W., Nelson, D. C. & Wirsen, C. O. (1989) Nature (London) 342, 834-836.
13. Nelson, D. C., Wirsen, C. O. & Jannasch, H. W. (1989) Appl. Environ. Microbiol. 55, 2909-2917.
14. Muyzer, G., Teske, A. P., Wirsen, C. O. & Jannasch, H. W. (1995) Arch. Microbiol. 164, 165-172.
15. Jannasch, H. W., Wirsen, C. O., Molyneaux, S. J. & Langworthy, T. A. (1992) Appl. Environ. Microbiol. 58, 3472-3481.
16. Perler, F. B., Southworth, M. W., Wilbur, D. G. & Wallace, D. (1995) in Archaea: A Laboratory Manual, eds. Robb, F. T. & Place, A. R. (Cold Spring Harbor Lab. Press, Plainview, NY), Vol. 3, pp. 139-147.
17. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), 2nd Ed.
18. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990) Methods Enzymol. 185, 60-89.
19. Kunkel, R. A., Roberts, J. D. & Zakour, R. A. (1987) Methods Enzymol. 154, 367-382.
20. Kong, H., Kucera, R. B. & Jack, W. E. (1993) J. Biol. Chem. 268, 1965-1975.
21. Gutman, P. D. & Minton, K. W. (1993) Nucleic Acids Res. 21, 4406-4407.
22. Davis, E. O., Thangaraj, J. S., Brooks, P. C. & Colston, M. J. (1994) EMBO J. 13, 699-703.
23. Lambowitz, A. M. & Belfort, M. (1993) Annu. Rev. Biochem. 62, 587-622.
24. Gimble, F. S. & Thorner, J. (1992) Nature (London) 357, 301-306.