Isolation and purification of an acidic pullulanase type II from newly isolated Bacillus sp. US149

Enzyme and Microbial Technology

Volumn 33, Issue 5, 2003, Pages 720-724

  Roy, Amitava ^a   Messaoud, E Ben ^a   Bejar, S ^a  

^a CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

Author keywords

16S rRNA; Bacillus; Isopullulanase; Protein purification

Indexed keywords

CELL CULTURE; HYDROLYSIS; ION EXCHANGE; SIZE EXCLUSION CHROMATOGRAPHY; SOILS;

MOLECULAR MASS;

ENZYMES;

ALPHA GLUCOSIDASE; AMYLOSE; GLUCOSE; HEPTOSE; ISOMALTOSE; PULLULAN; PULLULAN 4 GLUCANOHYDROLASE; PULLULANASE; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; BACILLUS; BACILLUS NAGANOENSIS; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATION; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; GEL PERMEATION CHROMATOGRAPHY; GRAM POSITIVE BACTERIUM; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOIL; SUPERNATANT;

BACILLUS; BACILLUS NAGANOENSIS; BACILLUS SP.;

EID: 0041379426     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00212-6     Document Type: Article

References (22)

1. Sakano Y., Masuda N., Kobayashi T. Hydrolysis of pullulan by a novel nzyme from Aspergillus niger. Agric. Biol. Chem. 35:1971;971-973.
2. Bertoldo C., Duffner F., Jorgensen P.L., Antranikian G. Pullulanase type I from Fervidobacterium pennavorans ven: cloning, sequencing and expression of the gene and biochemical characterization of the recombinant enzyme. Appl. Environ. Microbiol. 65:1999;2084-2091.
3. Rudiger A., Jorgensen P.L., Antranikian G. Isolation and characterization of a heat stable Pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in E. coli. Appl. Environ. Microbiol. 61:1995;567-575.
4. Koch R., Canganella F., Hippem H., Jahnke K.D.A., Antranikian G. Purification and properties of a thermostable Pullulanase from a newly isolated thermophilic anaerobic bacterium Fervidobacterium pennavorans Ven5. Appl. Environ. Microbiol. 63:1997;1088-1094.
5. Ben Messaoud E., Ben Ammar Y., Mellouli L., Bejar S. Thermostable pullulanase type I from new isolated Bacillus thermoleovorans US105; cloning, sequencing and expression of the gene in E. coli. Enzyme Microb. Technol. 31:2002;827-832.
6. Erra-Pujada M., Chang-Pi-Hin F., Debeire P., Duchiron F., O'Donohue M.J. Purification and properties of the catalytic domain of the thermostable pullulanase type II from Thermococcus hydrothermalis. Biotechnol. Lett. 23:2001;1273-1277.
7. Dong G., Vielle C., Zeikus J.G. Cloning, sequencing and expression of the gene encoding amylopullulanase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme. Appl. Environ. Microbiol. 63:1997;3577-3584.
8. Kim T.J., Kim M.J., Kim B.C., Kim J.C., Cheong T.K., Kim J.W.et al. Modes of the action of acarbose hydrolysis and transglycolysation catalysed by a thermostable maltogenic amylase, the gene of which was cloned from a Thermus strain. Appl. Environ. Microbiol. 56:1999;1644-1651.
9. Imanaka T., Kuriki T. Pattern of action of Bacillus stearothermophilus Neopullulanase on pullulan. J. Bacteriol. 171:1989;369-371.
10. Duffner F., Bertoldo C., Andersen J.T., Wagner K., Antranikian G. A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification and characterization of the recombinant enzyme after expression in Bacillus subtilis. J. Bacteriol. 182:2000;6331-6338.
11. Aoki H., Sakano Y. A classification of dextran-hydrolyzing enzyme based on amino-acid sequence similarities. Biochem. J. 323:1997;859-861.
12. Sakano Y., Taniguchi H., Okada G., Ohnishi M. Isopullulanse from Aspergillus niger. Tampakushits Kakusan Koso. 30(10):1985;1130-1134.
13. Aoki H., Sakano Y. Molecular cloning and heterologous expression of the isopullulanse gene from Aspergillus niger ATCC 9642. Biochem. J. 323:1997;757-764.
14. Voller G., Vilma A.S. New approaches to typing and identification of bacteria using the 16S-23S rDNA space region. Microbiology. 142:1996;3-16.
15. Mellouli L., Ghorbel R., Kammoun A., Mezghani M., Bejar S. Characterization of molecular cloning of the thermostable alpha-amylase from Streptomyces sp. T01. Biotechnol. Lett. 18:1996;809-814.
16. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. New York: Cold Spring Harbour Laboratory Press; 1989.
17. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74:1977;5463-5467.
18. Laemmeli U.K. Cleavage of structure protein during assembly of head of bacteriophage T4. Nature. 227:1970;680-685.
19. Miller G.L. Use of dinitrosalycilic acid reagent for determination of reducing sugar. Anal. Chem. 31:1959;426-428.
20. Goto K., Omura T., Hara Y., Sadaie Y. Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus. J. Gen. Appl. Microbiol. 46:2000;1-8.
21. Tomimura E., Zeman N.W., Frankiewicz J.R., Teague W.M. Description of Bacillus naganoensis sp. nov. Int. J. Syst. Bacteriol. 40:1990;123-125.
22. Aoki H., Yopi Padmajanti A., Sakano Y. Two components of cell-bound isopullulanase from Aspergillus niger ATCC 9642 - their purification and enzymatic properties. Biosci. Biotech. Biochem. 60:1996;1795-1798.