Conversion of the maltogenic ±-amylase Novamyl into a CGTase

Protein Engineering

Volumn 13, Issue 7, 2000, Pages 509-513

  Beier, Lars ^a   Svendsen, Allan ^a   Andersen, Carsten ^a   Frandsen, Torben P ^a   Borchert, Torben V ^a   Cherry, Joel R ^a  

^a NOVO NORDISK A S   (Denmark)

Author keywords

Amylase; CGTase; Product specificity; Site directed mutagenesis; Structural homology

Indexed keywords

AMINO ACID; AMYLASE; CYCLODEXTRIN; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; MALTOSE; NOVAMYL; STARCH; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYCLIZATION; ENZYME STRUCTURE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; THERMOSTABILITY; X RAY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACILLUS; BACTERIAL PROTEINS; BETA-CYCLODEXTRINS; BINDING SITES; CYCLODEXTRINS; GLUCOSYLTRANSFERASES; GLYCOSIDE HYDROLASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SEQUENCE HOMOLOGY, AMINO ACID; STARCH;

EID: 0033867376     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Christophersen, C., Otzen, D.E., Norman, B.E., Christensen, S. and Schäfer, T. (1998) Starch/Stärke, 50, 39-45.
2. Coutinho, P.M. and Henrissat, B. (1999) Carbohydrate-Active Enzymes server at URL: http://afmb.cnrs-mrs.fr/pedro/CAZY/db.html.
3. Dauter, Z., Dauter, M., Brzozowski, A.M., Christensen, S., Borchert, T.V., Beier, L., Wilson, K.S. and Davies, G.J. (1999) Biochemistry, 38, 8385-8392.
4. Diderichsen, B. and Christiansen, L. (1988) FEMS Micrrobiol. Lett., 56, 53-60.
5. Henrissat, B. (1991) Biochem. J., 280, 309-316.
6. Henrissat, B. and Bairoch, A. (1993) Biochem. J., 316, 695-696.
7. Higuchi, R., Krummel, B. and Saiki, R.K. (1988) Nucleic Acids Res., 16, 7351-7367.
8. Ibuka, A., Tonozuka, T., Matsuzawa, H. and Sakai, H. (1998) J. Biochem. (Tokyo), 123, 275-282.
9. Kuroki, R., Weaver, L.H. and Matthews, B.W. (1995) Nature Struct. Biol., 2, 1007-1011
10. Lawson, C.L., van Montfort, R., Strokopytov, B., Rozeboom, H.J., Kalk, K.H., de Vries, G.E., Penninga, D., Dijkhuizen, L. and Dijkstra, B.W. (1994) J. Mol. Biol., 236, 590-600.
11. Outtrup, H. and Norman, B.E. (1984) Starch/Stärke, 12, 405-411.
12. Penninga, D., Strokopytov, B., Rozeboom, H.J., Lawson, C.L., Dijkstra, B.W., Bergsma, J. and Dijkhuizen, L. (1995) Biochemistry, 34, 3368-3376.
13. Svensson, B. (1994) Plant Mol. Biol., 25, 141-157.
14. Vikmon, M. (1982) in Szejtli, J. (ed.), First International Symposium on Cyclodextrins, Budapest. Reidel, Dordrecht, pp. 69-74.
15. Wind, R.D., Buitelaar, R.M. and Dijkhuizen, L. (1998) Eur. J. Biochem., 253, 598-605
16. Zhang, J.H., Dawes, G. and Stemmer, W.P. (1997) Proc. Natl Acad. Sci. USA, 94, 4504-4509.