Evidence for importin α independent nuclear translocation of glucocorticoid receptors in Xenopus laevis Oocytes

Cellular Physiology and Biochemistry

Volumn 14, Issue 4-6, 2004, Pages 343-350

  Albermann, Lars ^a   Shahin, Victor ^a   Ludwig, Yvonne ^a   Schäfer, Claudia ^a   Schillers, Hermann ^a   Oberleithner, Hans ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Atomic force microscopy; Nuclear envelope; Nuclear localization signal; Nuclear pore complex

Indexed keywords

CHAPERONE; GLUCOCORTICOID; GLUCOCORTICOID RECEPTOR; KARYOPHERIN ALPHA; KARYOPHERIN BETA; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; ATOMIC FORCE MICROSCOPY; CELL NUCLEUS MEMBRANE; CELL STIMULATION; CONTROLLED STUDY; CYTOPLASM; FEMALE; MACROMOLECULE; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; OOCYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; WESTERN BLOTTING; XENOPUS LAEVIS;

XENOPUS LAEVIS;

EID: 4444356292     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000080344     Document Type: Article

References (39)

1. Hache RJ, Savory JG, Lefebvre YA: Nucleocytoplasmic trafficking and glucocorticoid receptor function. Gene Ther Mol Biol 1999;4:99-107.
2. Evans RM: The steroid and thyroid hormone receptor superfamily. Science 1988;240:889-895.
3. Carson-Jurica MA, Schrader WT, O'Malley BW: Steroid receptor family: structure and functions. Endocr Rev 1990;11:201-220.
4. Pratt WB and Toft DO: Steroid receptor interactions with heat shock protein and immunophilin chaperones. Endocr Rev 1997;18:306-360.
5. Yang J and Defranco DB: Assessment of glucocorticoid receptor-heat shock protein 90 interactions in vivo during nucleocytoplasmic trafficking. Mol Endocrinol 1996;10:3-13.
6. Pratt WB and Toft DO: Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery. Exp Biol Med (Maywood) 2003;228:111-133.
7. Galigniana MD, Scruggs JL, Herrington J, Welsh MJ, Carter-Su C, Housley PR, Pratt WB: Heat shock protein 90-dependent (geldanamycin-inhibited) movement of the glucocorticoid receptor through the cytoplasm to the nucleus requires intact cytoskeleton. Mol Endocrinol 1998;12:1903-1913.
8. Htun H, Barsony J, Renyi I, Gould DL, Hager GL: Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera. Proc Natl Acad Sci U S A 1996;93:4845-4850.
9. Defranco DB: Role of molecular chaperones in subnuclear trafficking of glucocorticoid receptors. Kidney Int 2000;57:1241-1249.
10. Görlich D: Transport into and out of the cell nucleus. EMBO J 1998;17:2721-2727.
11. Panté N and Kann M: Nuclear pore complex is able to transport macromolecules with diameters of about 39 nm. Mol Biol Cell 2002;13:425-434.
12. Görlich D and Kutay U: Transport between the cell nucleus and the cytoplasm. Annu Rev Cell Dev Biol 1999;15:607-660.
13. Xu L and Massague J: Nucleocytoplasmic shuttling of signal transducers. Nat Rev Mol Cell Biol 2004;5:209-219.
14. Görlich D, Kostka S, Kraft R, Dingwall C, Laskey RA, Hartmann E, Prehn S: Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope. Curr Biol 1995;5:383-392.
15. Moore JD, Kornbluth S, Hunt T: Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. Mol Biol Cell 2002;13:4388-4400.
16. Görlich D, Mattaj IW: Nucleocytoplasmic transport. Science 1996;271:1513-1518.
17. Tang Y, Ramakrishnan C, Thomas J, Defranco DB: A role for HDJ-2/HSDJ in correcting subnuclear trafficking, transactivation, and transrepression defects of a glucocorticoid receptor zinc finger mutant. Mol Biol Cell 1997;8:795-809.
18. Savory JG, Hsu B, Laquian IR, Giffin W, Reich T, Hache RJ, Lefebvre YA: Discrimination between NL1- and NL2-mediated nuclear localization of the glucocorticoid receptor. Mol Cell Biol 1999;19:1025-1037.
19. Tanaka M, Nishi M, Morimoto M, Sugimoto T, Kawata M: Yellow fluorescent protein-tagged and cyan fluorescent protein-tagged imaging analysis of glucocorticoid receptor and importins in single living cells. Endocrinology 2003;144:4070-4079.
20. Freedman ND, Yamamoto KR: Importin 7 and Importin alpha/Importin beta Are Nuclear Import Receptors for the Glucocorticoid Receptor. Mol Biol Cell 2004;15:2276-2286.
21. Schäfer C, Shahin V, Albermann L, Hug MJ, Reinhardt J, Schillers H, Schneider SW, Oberleithner H: Aldosterone signaling pathway across the nuclear envelope. Proc Natl Acad Sci U S A 2002;99:7154-7159.
22. Belikov S, Gelius B, Almouzni G, Wrange O: Hormone activation induces nucleosome positioning in vivo. EMBO J 2000;19:1023-1033.
23. Rakowska A, Danker T, Schneider SW, Oberleithner H: ATP-Induced shape change of nuclear pores visualized with the atomic force microscope. J Membr Biol 1998;163:129-136.
24. Görlich D, Vogel F, Mills AD, Hartmann E, Laskey RA: Distinct functions for the two importin subunits in nuclear protein import. Nature 1995;377:246-248.
25. Schillers H, Shahin V, Albermann L, Schäfer C, Oberleithner H: Imaging CFTR: A Tail to Tail Dimer with a Central Pore. Cell Physiol Biochem 2004;14:1-10.
26. Belikov S, Gelius B, Wrange O: Hormone-induced nucleosome positioning in the MMTV promoter is reversible. EMBO J 2001;20:2802-2811.
27. Oberleithner H, Brinckmann E, Giebisch G, Geibel J: Visualizing life on biomembranes by atomic force microscopy. Kidney Int 1995;48:923-929.
28. Henderson RM, Oberleithner H: Pushing, pulling, dragging, and vibrating renal epithelia by using atomic force microscopy. Am J Physiol Renal Physiol 2000;278:F689-F701.
29. Schillers H, Danker T, Madeja M, Oberleithner H: Plasma membrane protein clusters appear in CFTR-expressing Xenopus laevis oocytes after cAMP stimulation. J Membr Biol 2001;180:205-212.
30. Oberleithner H, Schäfer C, Shahin V, Albermann L: Route of steroid-activated macromolecules through nuclear pores imaged with atomic force microscopy. Biochem Soc Trans 2003;31:71-75.
31. Howell GM, Gustafsson JA, Lefebvre YA: Glucocorticoid receptor identified on nuclear envelopes of male rat livers by affinity labeling and immunochemistry. Endocrinology 1990;127:1087-1096.
32. Gelius B, Wrange O: Glucocorticoid hormone-induced receptor localization to the chromatin fibers formed on injected DNA in Xenopus oocytes. Exp Cell Res 2001;265:319-328.
33. Shahin V, Albermann L, Schillers H, Kastrup L, Schäfer C, Ludwig Y, Stock C, Oberleithner, H.: Steroids dilate nuclear pores imaged with atomic force microscopy. J Cell Physiol 2004; in press.
34. Ribbeck K and Görlich D: Kinetic analysis of translocation through nuclear pore complexes. EMBO J 2001;20:1320-1330.
35. Keminer O, Siebrasse JP, Zerf K, Peters R: Optical recording of signal-mediated protein transport through single nuclear pore complexes. Proc Natl Acad Sci U S A 1999;96:11842-11847.
36. Danker T, Schillers H, Storck J, Shahin V, Kramer B, Wilhelmi M, Oberleithner H: Nuclear hourglass technique: an approach that detects electrically open nuclear pores in Xenopus laevis oocyte. Proc Natl Acad Sci U S A 1999;96:13530-13535.
37. Danker T, Shahin V, Schlune A, Schäfer C, Oberleithner H: Electrophoretic plugging of nuclear pores by using the nuclear hourglass technique. J Membr Biol 2001;184:91-99.
38. Kutay U, Bischoff FR, Kostka S, Kraft R, Görlich D: Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell 1997;90:1061-1071.
39. Picard D and Yamamoto KR: Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor. EMBO J 1987;6:3333-3340.