Kinetic studies and site-directed mutagenesis of Escherichia coli agmatinase. a role for Glu274 in binding and correct positioning of the substrate guanidinium group

Archives of Biochemistry and Biophysics

Volumn 430, Issue 2, 2004, Pages 185-190

  Carvajal, Nelson ^a   Orellana, María S ^a   Salas, Mónica ^a   Enríquez, Paula ^a   Alarcón, Ricardo ^a   Uribe, Elena ^a   López, Vasthi ^a  

^a UNIVERSITY OF CONCEPCIÓN   (Chile)

Author keywords

Agmatinase; E. coli; Glu274; Kinetic mechanism; Site directed mutagenesis

Indexed keywords

AGMATINE; AMINO ACID DERIVATIVE; BACTERIAL ENZYME; GUANIDINE; HYDROLASE; PUTRESCINE; TRYPTOPHAN;

AMINO ACID SEQUENCE; CATALYSIS; CIRCULAR DICHROISM; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME BINDING; ESCHERICHIA COLI; LINEAR SYSTEM; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STATISTICAL SIGNIFICANCE; STRUCTURE ANALYSIS; WILD TYPE;

BINDING SITES; CATALYSIS; ESCHERICHIA COLI; GLUTAMINE; GUANIDINE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; UREOHYDROLASES; VARIATION (GENETICS);

ESCHERICHIA COLI;

EID: 4444281756     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.abb.2004.07.005     Document Type: Conference Paper

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