Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling: A critical role for aspartate 153

European Journal of Biochemistry

Volumn 269, Issue 22, 2002, Pages 5522-5526

  Salas, Mónica ^a   Rodríguez, Rolando ^b   López, Nelia ^b   Uribe, Elena ^a   López, Vasthi ^a   Carvajal, Nelson ^a  

^a UNIVERSITY OF CONCEPCIÓN   (Chile)

^b CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Agmatinase; Asp153; E. coli; Homology modelling; Site directed mutagenesis

Indexed keywords

AGMANTINASE; AGMATINE; ASPARAGINE; ASPARTIC ACID; CARBON; GUANIDINE; HYDROXIDE; METAL ION; PUTRESCINE; TRYPTOPHAN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; FLUORESCENCE; HYDROGEN BOND; MICHAELIS CONSTANT; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN STRUCTURE; REGULATORY MECHANISM; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; ARGINASE; ASPARTIC ACID; CIRCULAR DICHROISM; EDETIC ACID; ESCHERICHIA COLI; KINETICS; LIVER; MANGANESE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RATS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, FLUORESCENCE; UREOHYDROLASES;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036436957     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03255.x     Document Type: Article

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