A tyrosine substitution in the cavity wall of a K channel induces an inverted inactivation

Biophysical Journal

Volumn 94, Issue 8, 2008, Pages 3014-3022

  Klement, Göran ^a   Nilsson, Johanna ^a   Århem, Peter ^a   Elinder, Fredrik ^b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b LINKÖPING UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

KCNH2 PROTEIN, HUMAN; POTASSIUM CHANNEL HERG; TYROSINE;

AMINO ACID SUBSTITUTION; ANIMAL; ARTICLE; BIOLOGICAL MODEL; CELL CULTURE; CHANNEL GATING; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; METABOLISM; OOCYTE; PHYSIOLOGY; POROSITY; PROTEIN CONFORMATION; ULTRASTRUCTURE; XENOPUS LAEVIS;

AMINO ACID SUBSTITUTION; ANIMALS; CELLS, CULTURED; COMPUTER SIMULATION; ETHER-A-GO-GO POTASSIUM CHANNELS; ION CHANNEL GATING; MODELS, BIOLOGICAL; MODELS, CHEMICAL; MODELS, MOLECULAR; OOCYTES; POROSITY; PROTEIN CONFORMATION; TYROSINE; XENOPUS LAEVIS;

EID: 43149112334     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1529/biophysj.107.119842     Document Type: Article

References (35)

1. Armstrong, C. M. 1971. Interaction of tetraethylammonium ion derivatives with the potassium channels of giant axons. J. Gen. Physiol. 58:413-437.
2. + conduction and selectivity. Science. 280:69-77.
3. Melishchuk, A., and C. M. Armstrong. 2001. Mechanism underlying slow kinetics of the OFF gating current in Shaker potassium channel. Biophys. J. 80:2167-2175.
4. + channels: evidence for a trap door mechanism of activation gating. J. Gen. Physiol. 109:527-535.
5. + channel. J. Gen. Physiol. 119:521-532.
6. + channel structure and its implications for neuronal channels. Curr. Opin. Neurobiol. 9:267-273.
7. Sanguinetti, M. C., C. Jiang, M. E. Curran, and M. T. Keating. 1995. A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel. Cell. 81:299-307.
8. Smith, P. L., T. Baukrowitz, and G. Yellen. 1996. The inward rectification mechanism of the HERG cardiac potassium channel. Nature. 379:833-836.
9. Spector, P. S., M. E. Curran, A. Zou, M. T. Keating, and M. C. Sanguinetti. 1996. Fast inactivation causes rectification of the IKr channel. J. Gen. Physiol. 107:611-619.
10. Schonherr, R., and S. H. Heinemann. 1996. Molecular determinants for activation and inactivation of HERG, a human inward rectifier potassium channel. J. Physiol. 493:635-642.
11. Chen, J., G. Seebohm, and M. C. Sanguinetti. 2002. Position of aromatic residues in the S6 domain, not inactivation, dictates cisapride sensitivity of HERG and eag potassium channels. Proc. Natl. Acad. Sci. USA. 99:12461-12466.
12. Jiang, Y., A. Lee, J. Chen, M. Cadene, B. T. Chait, and R. MacKinnon. 2002. Crystal structure and mechanism of a calcium-gated potassium channel. Nature. 417:515-522.
13. Chanda, B., O. K. Asamoah, R. Blunck, B. Roux, and F. Bezanilla. 2005. Gating charge displacement in voltage-gated ion channels involves limited transmembrane movement. Nature. 436:852-856.
14. Kamb, A., L. E. Iverson, and M. A. Tanouye. 1987. Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel. Cell. 50:405-413.
15. Hoshi, T., W. N. Zagotta, and R. W. Aldrich. 1990. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 250:533-538.
16. + channel. J. Gen. Physiol. 112:377-389.
17. Elinder, F., R. Männikkö, and H. P. Larsson. 2001. S4 charges move close to residues in the pore domain during activation in a K channel. J. Gen. Physiol. 118:1-10.
18. Klemic, K. G., G. E. Kirsch, and S. W. Jones. 2001. U-type inactivation of Kv3.1 and Shaker potassium channels. Biophys. J. 81:814-826.
19. Timpe, L. C., T. L. Schwarz, B. L. Tempel, D. M. Papazian, Y. N. Jan, and L. Y. Jan. 1988. Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes. Nature. 331:143-145.
20. Hodgkin, A. L., and A. F. Huxley. 1952. The dual effect of membrane potential on sodium conductance in the giant axon of Loligo. J. Physiol. 116:497-506.
21. Kurata, H. T., and D. Fedida. 2006. A structural interpretation of voltage-gated potassium channel inactivation. Prog. Biophys. Mol. Biol. 92:185-208.
22. + channels in human T lymphocytes. Biophys. J. 55:203-206.
23. Lopez-Barneo, J., T. Hoshi, S. H. Heinemann, and R. W. Aldrich. 1993. Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels. Receptors Channels. 1:61-71.
24. + channels. Proc. Natl. Acad. Sci. USA. 88:5092-5095.
25. Schoppa, N. E., K. McCormack, M. A. Tanouye, and F. J. Sigworth. 1992. The size of gating charge in wild-type and mutant Shaker potassium channels. Science. 255:1712-1715.
26. Zhou, M., J. H. Morais-Cabral, S. Mann, and R. MacKinnon. 2001. Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors. Nature. 411:657-661.
27. + channels: effects of alterations in the carboxyterminal region. Neuron. 7:547-556.
28. Cordero-Morales, J. F., L. G. Cuello, Y. Zhao, V. Jogini, D. M. Cortes, B. Roux, and E. Perozo. 2006. Molecular determinants of gating at the potassium-channel selectivity filter. Nat. Struct. Mol. Biol. 13:311-318.
29. Klemic, K. G., C. C. Shieh, G. E. Kirsch, and S. W. Jones. 1998. Inactivation of Kv2.1 potassium channels. Biophys. J. 74:1779-1789.
30. + channels. J. Biol. Chem. 277:29045-29053.
31. Olcese, R., D. Sigg, R. Latorre, F. Bezanilla, and E. Stefani. 2001. A conducting state with properties of a slow inactivated state in a Shaker K(+) channel mutant. J. Gen. Physiol. 117:149-163.
32. + solutions: role of the voltage sensor. Biophys. J. 75:1828-1835.
33. + ions: a remarkably stable non-conducting state produced by membrane depolarizations. J. Physiol. 499:3-15.
34. +. Biophys. J. 80:2704-2714.
35. + channels. J. Mol. Biol. 354:272-288.