A protocol for immunoaffinity separation of the accumulated ubiquitin-protein conjugates solubilized with sodium dodecyl sulfate

Analytical Biochemistry

Volumn 377, Issue 1, 2008, Pages 77-82

  Shimada, Yohta ^a   Fukuda, Takahiro ^b   Aoki, Katsuhiko ^a   Yukawa, Toyokazu ^a   Iwamuro, Shawichi ^c   Ohkawa, Kiyoshi ^a   Takada, Koji ^a  

^a JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b Research Center for Medical Science   (Japan)

^c TOHO UNIVERSITY   (Japan)

Author keywords

Cycloamylose; Flotillin 1; Heat shock; Niemann Pick type C disease; NPC; SDS; Sodium dodecyl sulfate; Tween 20; Ubiquitin; Ubiquitin protein conjugate

Indexed keywords

ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; CYCLODEXTRINS; HISTOLOGY; NEURODEGENERATIVE DISEASES; SEPARATION; SULFUR COMPOUNDS; TISSUE;

FLOTILLIN-1; HEAT-SHOCK; NIEMANN-PICK TYPE C; PROTEIN CONJUGATES; TWEEN-20; UBIQUITIN;

SODIUM DODECYL SULFATE;

BRAIN EXTRACT; DODECYL SULFATE SODIUM; FLOTILLIN 1; PROTEIN; UBIQUITIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL STRAIN K 562; FEMALE; HUMAN; HUMAN CELL; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOPRECIPITATION; LIPID RAFT; MALE; MOUSE; NIEMANN PICK DISEASE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN RENATURATION; QUANTITATIVE ANALYSIS; SOLUBILIZATION;

EID: 42649095634     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.02.031     Document Type: Article

References (32)

1. M. Azarkan J. Huet D. Baeyens-Volant Y. Looze G. Vandenbussche Affinity chromatography: a useful tool in proteomics studies J. Chromatogr. B Anal. Technol. Biomed. Life. Sci. 849 2007 81 90
2. E. Harlow D. Lane Antibodies: A Laboratory Manual Cold 1988 Spring Harbor Laboratory Press New York
3. K. Takada T. Hirakawa H. Yokosawa Y. Okawa H. Taguchi K. Ohkawa Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques Biochem. J. 356 2001 199 206
4. T. Usuba Y. Ishibashi Y. Okawa T. Hirakawa K. Takada K. Ohkawa Purification and identification of monoubiquitin-phosphoglycerate mutase B complex from human colorectal cancer tissues Int. J. Cancer 94 2001 662 668
5. Y. Okawa K. Takada J. Minami K. Aoki H. Shibayama K. Ohkawa Purification of N-terminally truncated histone H2A-monoubiquitin conjugates from leukemic cell nuclei: probable proteolytic products of ubiquitinated H2A Int. J. Biochem. Cell. Biol. 35 2003 1588 1600
6. M. Matsumoto S. Hatakeyama K. Oyamada Y. Oda T. Nishimura K.I. Nakayama Large-scale analysis of the human ubiquitin-related proteome Proteomics 5 2005 4145 4151
7. J. Vasilescu J.C. Smith M. Ethier D. Figeys Proteomic analysis of ubiquitinated proteins from human MCF-7 breast cancer cells by immunoaffinity purification and mass spectrometry J. Proteome Res. 4 2005 2192 2200
8. M. Morishima-Kawashima M. Hasegawa K. Takio M. Suzuki K. Titani Y. Ihara Ubiquitin is conjugated with amino-terminally processed tau in paired helical filaments Neuron 10 1993 1151 1160
9. D.W. Dickson W. Liu J. Hardy M. Farrer N. Mehta R. Uitti M. Mark T. Zimmerman L. Golbe J. Sage A. Sima C. D’Amato R. Albin S. Gilman S.H. Yen Widespread alterations of alpha-synuclein in multiple system atrophy Am. J. Pathol. 155 1999 1241 1251
10. W.G. Johnson Late-onset neurodegenerative diseases--the role of protein insolubility J. Anat. 196 Pt 4 2000 609 616
11. H. Mori J. Kondo Y. Ihara Ubiquitin is a component of paired helical filaments in Alzheimer’s disease Science 235 1987 1641 1644
12. M.T. Vanier G. Millat Niemann-Pick disease type C Clin. Genet. 64 2003 269 281
13. S. Love L.R. Bridges C.P. Case Neurofibrillary tangles in Niemann-Pick disease type C Brain 118 Pt 1 1995 119 129
14. Y. Higashi S. Murayama P.G. Pentchev K. Suzuki Cerebellar degeneration in the Niemann-Pick type C mouse Acta Neuropathol. (Berl) 85 1993 175 184
15. H. Suzuki T. Sakiyama N. Harada M. Abe M. Tadokoro Pathologic changes of glial cells in murine model of Niemann-Pick disease type C: immunohistochemical, lectin-histochemical and ultrastructural observations Pediatr. Int. 45 2003 1 4
16. M. Fujimuro H. Sawada H. Yokosawa Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins FEBS Lett. 349 1994 173 180
17. S.K. Loftus J.A. Morris E.D. Carstea J.Z. Gu C. Cummings A. Brown J. Ellison K. Ohno M.A. Rosenfeld D.A. Tagle P.G. Pentchev W.J. Pavan Murine model of Niemann-Pick C disease: mutation in a cholesterol homeostasis gene Science 277 1997 232 235
18. S. Machida S. Ogawa S. Xiaohua T. Takaha K. Fujii K. Hayashi Cycloamylose as an efficient artificial chaperone for protein refolding FEBS Lett. 486 2000 131 135
19. J. Minami K. Takada K. Aoki Y. Shimada Y. Okawa N. Usui K. Ohkawa Purification and characterization of C-terminal truncated forms of histone H2A in monocytic THP-1 cells Int. J. Biochem. Cell Biol. 39 2007 171 180
20. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
21. J. Kyhse-Andersen Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose J. Biochem. Biophys. Methods 10 1984 203 209
22. P.S. Swerdlow D. Finley A. Varshavsky Enhancement of immunoblot sensitivity by heating of hydrated filters Anal. Biochem. 156 1986 147 153
23. K. Takada H. Nasu N. Hibi Y. Tsukada K. Ohkawa M. Fujimuro H. Sawada H. Yokosawa Immunoassay for the quantification of intracellular multi-ubiquitin chains Eur. J. Biochem. 233 1995 42 47
24. F. Rusconi E. Valton R. Nguyen E. Dufourc Quantification of sodium dodecyl sulfate in microliter-volume biochemical samples by visible light spectroscopy Anal. Biochem. 295 2001 31 37
25. P.E. Bickel P.E. Scherer J.E. Schnitzer P. Oh M.P. Lisanti H.F. Lodish Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins J. Biol. Chem. 272 1997 13793 13802
26. S.J. Lee U. Liyanage P.E. Bickel W. Xia P.T. Lansbury Jr. K.S. Kosik A detergent-insoluble membrane compartment contains A beta in vivo Nat. Med. 4 1998 730 734
27. Y. Tomidokoro T. Lashley A. Rostagno T.A. Neubert M. Bojsen-Moller H. Braendgaard G. Plant J. Holton B. Frangione T. Revesz J. Ghiso Familial Danish dementia: co-existence of Danish and Alzheimer amyloid subunits (ADan AND A{beta}) in the absence of compact plaques J. Biol. Chem. 280 2005 36883 36894
28. R. Tomazin J. Boname N.R. Hegde D.M. Lewinsohn Y. Altschuler T.R. Jones P. Cresswell J.A. Nelson S.R. Riddell D.C. Johnson Cytomegalovirus US2 destroys two components of the MHC class II pathway, preventing recognition by CD4+ T cells Nat. Med. 5 1999 1039 1043
29. C. Nagayoshi M. Ishibashi Y. Kita M. Matsuoka I. Nishimoto M. Tokunaga Expression, refolding and characterization of human brain serine racemase in Escherichia coli with N-terminal His-tag Protein Pept. Lett. 12 2005 487 490
30. E.T. Parkin A.J. Turner N.M. Hooper Amyloid precursor protein, although partially detergent-insoluble in mouse cerebral cortex, behaves as an atypical lipid raft protein Biochem. J. 344 Pt 1 1999 23 30
31. R.H. Ganderton K.K. Stanley C.E. Field M.P. Coghlan M.A. Soos K. Siddle A monoclonal anti-peptide antibody reacting with the insulin receptor beta-subunit. Characterization of the antibody and its epitope and use in immunoaffinity purification of intact receptors Biochem. J. 288 Pt 1 1992 195 205
32. W. Chen A. Nishikimi T. Kamata Y. Adachi A reducing and denaturing step maximizes the immunoprecipitations of m-calpain and I-2(PP2A)/SET: an approach toward antibodies that do not work well in immunoprecipitation J. Biochem. Biophys. Methods 68 2006 65 68