Expression, refolding and characterization of human brain serine racemase in Escherichia coli with N-terminal His-tag

Protein and Peptide Letters

Volumn 12, Issue 5, 2005, Pages 487-490

  Nagayoshi, Chizuru ^a   Ishibashi, Matsujiro ^a   Kita, Yoshiko ^b   Matsuoka, Masaaki ^b   Nishimoto, Ikuo ^b   Tokunaga, Masao ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b KEIO UNIVERSITY   (Japan)

Author keywords

Cycloamylose; D Ser; Detergents; Human brain serine racemase; NMDA receptor; Refolding

Indexed keywords

DETERGENT; ISOMERASE; RECOMBINANT PROTEIN; SERINE RACEMASE;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; BRAIN; CELL INCLUSION; ENZYMOLOGY; ESCHERICHIA COLI; EXPRESSED SEQUENCE TAG; GEL CHROMATOGRAPHY; GENE EXPRESSION PROFILING; HUMAN; MOLECULAR GENETICS; PROTEIN FOLDING; SOLUBILITY;

AMINO ACID SEQUENCE; BRAIN; CHROMATOGRAPHY, AGAROSE; DETERGENTS; ESCHERICHIA COLI; EXPRESSED SEQUENCE TAGS; GENE EXPRESSION PROFILING; HUMANS; INCLUSION BODIES; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; RACEMASES AND EPIMERASES; RECOMBINANT PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 21444432685     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/0929866054395284     Document Type: Article

References (16)

1. Nagata, Y., Konno, R., Yasumura, Y. and Akino, T. (1989) Biochem. J. 257, 291-292.
2. Hashimoto, A., Nishikawa, T., Hayashi, T., Fujii, N., Harada, K., Oka, T. and Takahashi, K. (1992) FEBS Lett. 296, 33-36.
3. Wolosker, H., Blackshaw, S. and Snyder, S. H. (1999) Proc. Natl. Acad Sci. USA 96, 13409-13414.
4. Mothet, J-P., Parent, A. T., Wolosker, H., Brady, Jr. R. O., Linden, D. J., Ferris, C.D., Rogawski, M. A. and Snyder, S. H. (2000) Proc. Natl. Acad. Sci. USA 97, 4926-4931.
5. Snyder, S. H. and Ferris, C. D. (2000) Am. J. Psychiatry 157, 1738-1751.
6. Panizzutti, R., Miranda, J. D. Ribeiro, C. S. Engelender, S. and Wolosker, H. (2001) Proc. Natl. Acad. Sci. USA 98, 5294-5299.
7. Wolosker, H., Sheth, K. N., Takahashi, M., Mothet, J-P., Brady, Jr. R. O., Ferris, C. D. and Snyder, S. H. (1999) Proc. Natl. Acad. Sci. USA 96, 721-725.
8. Miranda, J. D., Santoro, A., Engelender, S. and Wolosker, H. (2000) Gene 256, 183-188.
9. Kaneko, I., Morimoto, K. and Kubo, T. (2001) Neuroscience 104, 1003-1011.
10. Machida, S., Ogawa, S., Xiaohua, S., Takaha, T., Fujii, K. and Hayashi, K. (2000) FEBS Lett. 486, 131-135.
11. Laemmli, U.K. (1970) Nature 227, 680-685.
12. Tokunaga, M., Ishibashi, M., Tatsuda, D. and Tokunaga, H. (1997) Yeast 13, 699-706.
13. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) J. Biol. Chem. 193, 265-215.
14. Neidle, A. and Dunlop, D. S. (2002) Neurochem. Res. 27, 1719-1724.
15. Cook, S. P., Galve-Roperh, I., del Pozo, A. M. and Rodriguez-Crespo, I. (2002) J. Biol. Chem., 277, 27782-27792.
16. Strisovsky, K., Jiraskova, J., Barinka, C., Majer, P., Rojas, C., Slusher, B. S. and Konvalinka, J. (2003) FEBS Lett. 535, 44-48.