A reducing and denaturing step maximizes the immunoprecipitations of m-calpain and I-2PP2A/SET: An approach toward antibodies that do not work well in immunoprecipitation

Journal of Biochemical and Biophysical Methods

Volumn 68, Issue 1, 2006, Pages 65-68

  Chen, Wei ^a   Nishikimi, Akihiko ^b   Kamata, Tohru ^a   Adachi, Yoshifumi ^a,b  

^a SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Antibody; I 2PP2A SET; Immunoprecipitation; m Calpain

Indexed keywords

ANTIBODY; CALPAIN; ONCOPROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DENATURATION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; IMMUNOREACTIVITY; NONHUMAN; PRIORITY JOURNAL;

ANTIBODIES; CALPAIN; CELL LINE, TUMOR; HUMANS; IMMUNOPRECIPITATION;

EID: 33745008602     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2006.02.012     Document Type: Article

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