Optimization of the AT-content of codons immediately downstream of the initiation codon and evaluation of culture conditions for high-level expression of recombinant human G-CSF in Escherichia coli

Molecular Biotechnology

Volumn 38, Issue 3, 2008, Pages 221-232

  Krishna Rao, Dasari V ^a   Rao, Joginapally V ^b   Narasu, Mangamoori L ^b   Bhujanga Rao, Adibhatla Kali S ^a  

^a NATCO   (India)

^b JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY   (India)

Author keywords

AT rich codon; Codon optimization; High level expression; Recombinant human G CSF; Site directed mutagenesis; Specific product yield

Indexed keywords

CELL CULTURE; CELL GROWTH; CLONING; ESCHERICHIA COLI; GLUCOSE; GROWTH KINETICS; OPTIMIZATION; WATER POLLUTION;

AT-RICH CODON; CODON OPTIMIZATION; CULTURE CONDITIONS; E. COLI; GRANULOCYTE COLONY-STIMULATING FACTOR; HIGH-LEVEL EXPRESSION; HOST STRAIN; N-TERMINAL REGION; PROCESSING METHODS; RECOMBINANT HUMAN G-CSF; SILENT MUTATIONS; SITE-DIRECTED MUTAGENESIS; SPECIFIC PRODUCT YIELD;

ANIMAL CELL CULTURE;

CELL PROTEIN; GLUCOSE; ISOPROPYL THIOGALACTOSIDE; PLASMID VECTOR; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; RIFAMPICIN;

AMINO TERMINAL SEQUENCE; ARTICLE; AT RICH SEQUENCE; BACTERIAL CELL; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL GROWTH; CODON; CULTURE MEDIUM; ESCHERICHIA COLI; GENE MUTATION; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; BIOLOGICAL MODEL; DNA BASE COMPOSITION; DRUG EFFECT; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; PROTEIN SYNTHESIS; SITE DIRECTED MUTAGENESIS; START CODON;

ESCHERICHIA COLI;

BASE COMPOSITION; CODON; CODON, INITIATOR; ESCHERICHIA COLI; GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT; HUMANS; MODELS, GENETIC; MUTAGENESIS, SITE-DIRECTED; PROTEIN BIOSYNTHESIS; RIFAMPIN;

EID: 41549150394     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-9018-3     Document Type: Article

References (40)

1. M. Tamura K. Hattori H. Nomura M. Oheda N. Kubota I. Imazeki M. Ono Y. Ueyama S. Nagata N. Shirafuji 1987 Induction of neutrophilic granulocytosis in mice by administration of purified human native granulocyte colony stimulating factor (G-CSF) Biochemical and Biophysical Research Communication 142 454 460
2. S. H. Nagata M. Tsuchiya S. Asano O. Yamamato Y. Hirata N. Kubota M. Oheda H. Nomura T. Yamazaki 1986 The chromosomal gene structure and two mRNAs for human granulocyte colony stimulating factor EMBO Journal 5 575 581
3. S. H. Nagata R. Fukunaga 1991 Granulocyte colony stimulating factor and itsreceptor Progress in Growth Factor Research 3 131 141
4. G. Morstyn L. Campbell L. M. Souza N. K. Alton J. Keech M. Green W. Sheridhan D. Metcalf R. Fox 1988 Effect of granulocyte colony stimulating factor on neutropenia induced by cytotoxic chemotherapy Lancet 1 667 672
5. L. M. Souza T. C. Boone J. Gabrilove P. H. Lai K. M. Zsebo D. C. Murdock V. R. Chazin J. Bruszewski H. Lu K. K. Chen 1986 Recombinant human granulocyte colony stimulating factor; effects on normal and leukemia myloid cells Science 232 61 65
6. H. Teshima J. Ishikawa H. Kitayama T. Yamagami A. Hiraoka H. Nakamura H. Shibata T. Masaoka F. Takaku 1989 Clinical effects of recombinant human granulocyte colony stimulating factor in leukemia patients: a phase I/II study Experimental Hematology 17 853 858
7. M. Hoglund 1998 Glycosylated and non-glycosylated recombinant hG-CSF, What is the difference? Medical Oncology 15 229 233
8. S. Nagata M. Tsuchiya S. Asano Y. Kaziro T. Yamazaki O. Yamamoto Y. Hitata N. Kubota M. Oheda H. Nomura M. Ono 1986 Molecular cloning and expression of cDNA for human granulocyte colony stimulating factor Nature 319 415 418
9. Y. Komastu T. Matsumoto T. Kuga T. Nishi S. Sekine A. Satio M. Okabe M. Morimoto S. Itoh T. Okabe 1987 cloning of granulocyte colony stimulating factor cDNA and its expression in Escherichia coli Japanese Journal of Cancer Research 78 1179 1181
10. M. J. Weickert D. H. Doherty E. A. Best P. O. Olins 1996 Optimization of heterologous protein production in Escherichia coli Current Opinion in Biotechnology 7 494 499
11. F. Baneyx 1999 Recombinant protein expression in Escherichia coli Current Opinion in Biotechnology 10 411 421
12. G. Hannig S. C. Makrides 1998 Strategies for optimizing heterologous protein expression in Escherichia coli Trends in Biotechnology 16 54 60
13. J. F. Kane 1995 Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli Current Opinion in Biotechnology 6 494 500
14. G. T. Chen M. Inouye 1994 Role of AGA/AGG codons the rarest codons in global gene expression in Escherichia coli Genes and Development 8 2641 2652
15. R. S. Hale G. Thompson 1998 Codon optimization of the gene encoding a domain from the human type 1 neurofibromin protein results in three fold improvement in expression level in Escherichia coli Protein Expression and Purification 12 185 188
16. X. Hu Q. Shi T. Yang G. Jackowski 1996 Specific replacement of consecutive AGG codons results in high level expression of human cardiac troponin T in Escherichia coli Protein Expression and Purification 7 289 293
17. P. E. Devlin R. J. Drummond P. Toy D. F. Mark K. W. K. Watt J. J. Devlin 1988 Alteration of amino-terminal codons of human granulocyte-colony-stimulating factor increases expression levels and allows efficient processing by methionine aminopeptidase in Escherichia coli Gene 65 13 22
18. Z. H. Shu Y. Qinong 1998 Expression of cDNA for rhG-CSF in E. coli and characterization of the protein Chinese Journal of Cancer Research 10 256 259
19. S. H. Kang K. H. Na J. H. Park C. Park S. Y. Lee Y. I. Lee 1995 High-level expression and simple purification of recombinant human granulocyte colony stimulating factor in E. coli Biotechnology Letter 17 687 692
20. M. J. Fallah B. Akbari A. R. Saeedinia M. Karimi M. vaez M. Zeinoddini M. Soleimani M. Maghsoudi 2003 Over expression of recombinant human granulocyte colony stimulating factor in E. coli I J M S 28 131 134
21. A. Saeedinia M. Sadeghizadeh N. Maghsoudi B. Akbari J. Fallah M. Karimi 2002 Construction and cloning of humangranulocyte cology stimulating factor (hG-CSF) cDNA Modarres J M S Spring-Summer 5 55 64
22. A. Jacobson 1987 Purification and fractionation of Poly A + RNA Methods in Enzymology 152 254 261
23. K. Weber J. R. Pringle M. Osborn 1972 Measurement of molecular weights by electrophoresis on SDS-acrylamide gel Methods in Enzymology 26 3 27
24. Helmut, B., Hildburg, B., & Gross (1987). Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophorisis, 8, 93-99.
25. M. Kubo T. Imanaka 1989 mRNA secondary structure in an open reading frame reduces translation efficiency in Bacillus subtilis Journal of Bacteriology 171 4080 4082
26. S. A. Liebhaber F. Cash S. S. Eshleman 1992 Translation inhibition by an mRNA coding region secondary structure is determined by its proximity to the AUG initiation codon Journal of Molecular Biology 226 609 621
27. D. Voges M. Watzele C. Nemetz S. Wizemann B. Buchberger 2004 Analyzing and enhancing mRNA translational efficiency in an Escherichia coli in vitro expression system Biochemical and Biophysical Research Communication 318 601 614
28. G. Qing B. Xia M. Inoute 2003 Enhancement of translation initiation by AT-rich sequences downstream of the initiation codon in Escherichia coli Journal of Molecular Microbiology and Biotechnology 6 133 144
29. P. Srivastava P. Bhattacharaya G. Pandey K. J. Mukarjee 2005 Over expression and purification of recombinant human interferon alpha2b in Escherichia coli Protein Expression and Purification 41 313 322
30. F. W. Studier 2005 Protein production by auto induction in high density shaking cultures Protein Expression and Purificaion 41 207 234
31. T. H. Grossman E. S. Kawasaki 1998 Spontaneous cAMP dependent de repression of gene expression in stationary phase plays a role in recombinant expression instability Gene 209 95 103
32. H. Holms 1996 Flux analysis and control of the central metabolic pathways in Escherichia coli FEMS Microbial Review 19 85 116
33. S Pan B. A. Malcolm 2000 Reduced background expression and improved plasmid stability with PET vectors in BL 21 (DE3) Biotechniques 29 1234 1238
34. R. Khalilzadeh S. A. Shojaosadati A. Bahrami N. Maghsoudi 2003 Over expression of recombinant human interferon-gamma in high cell density fermentation of Escherichia coli Biotechnology Letter 25 1989 1992
35. A. A. Travers R. R. Burges 1969 Cyclic reuse of RNA polymerase sigma factor Nature 222 537 540
36. A. Kuderova E Nanak M. Truksa B. Brzobohaty 1999 Use of Rifampicin in T7 RNA polymerase driven expression of a Plant enzyme: Rifampicin improves yield and assembly Protein Expression and Purification 16 405 409
37. J. G. Thomas F. Baneyx 1996 Production of misfolding and induction formation in recombinant Ecsherichia coli cells overexpressing heat shock protein Journal of Biological Chemistry 271 11141 11147
38. F. Hoffmann U. Rinas 2004 Role of heat shock protein in the production of recombinant proteins in Escherichia coli Advances in Biochemical Engineering and Biotecnology 89 143 161
39. M. K. Oh J. C. Liao 2000 DNA microarray detection of metabolic responces to protein over production in Escherichia coli Metabolic Engineering 2 201 209
40. R. Gill M. Delisa J. Valdes W. Bentlay 2000 Genomic analysis of high cell density recombinant Escherichia coli fermentation and "cell conducting" for improved recombinant protein yield Biotechnology and.Bioengineering 72 85 95