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Volumn 112, Issue 9, 2008, Pages 2622-2628

Cholesterol location and orientation in aqueous suspension of large unilamellar vesicles of phospholipid revealed by intermolecular nuclear overhauser effect

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEAR MAGNETIC RESONANCE; PHOSPHOLIPIDS; PROTONS; SUSPENSIONS (FLUIDS);

EID: 41549138448     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp0760713     Document Type: Article
Times cited : (14)

References (61)
  • 32
    • 84906363735 scopus 로고    scopus 로고
    • The extrusion was performed until the solution became transparent. At a higher cholesterol concentration, a larger number of passages were required for the solution to become transparent because of the cholesterol-induced rigidity of the bilayer membrane
    • The extrusion was performed until the solution became transparent. At a higher cholesterol concentration, a larger number of passages were required for the solution to become transparent because of the cholesterol-induced rigidity of the bilayer membrane.
  • 34
    • 84906391989 scopus 로고    scopus 로고
    • It should be noted that here we show the NOE enhancement by (I(t m, I0]/I0, rather than Itm, I0, because the former represents the flux of intermolecular magnetization transfer between a specific pair of sites, whereas the latter, related to the cross relaxation rate, is proportional to the number of protons that receive magnetizations. The selective π pulse was applied for the choline group of PC. We do not explicitly take the intensity of the selectively inverted choline signal into account when we show the NOE enhancement, since the amount of PC was kept constant and the linewidth of the choline peak is not sensitive to the cholesterol content
    • 0, because the former represents the flux of intermolecular magnetization transfer between a specific pair of sites, whereas the latter, related to the cross relaxation rate, is proportional to the number of protons that receive magnetizations. The selective π pulse was applied for the choline group of PC. We do not explicitly take the intensity of the selectively inverted choline signal into account when we show the NOE enhancement, since the amount of PC was kept constant and the linewidth of the choline peak is not sensitive to the cholesterol content.
  • 35
    • 84906363736 scopus 로고    scopus 로고
    • 2O in pure solvent (ref 33).
    • 2O in pure solvent (ref 33).
  • 37
    • 84906363737 scopus 로고    scopus 로고
    • For the fwhh and chemical shift measurements at cholesterol concentrations of 1 mol%, a new probe for a sample tube of 20 mm diameter (JEOL ECA500W) was used.
    • For the fwhh and chemical shift measurements at cholesterol concentrations of 1 mol%, a new probe for a sample tube of 20 mm diameter (JEOL ECA500W) was used.
  • 49
    • 84906391987 scopus 로고    scopus 로고
    • The interdigitation (for a review, see ref 46) or the interpenetration of lipids into the other leaflet of the bilayer may be another possible reason for the close hydrophilic/hydrophobic contact
    • The interdigitation (for a review, see ref 46) or the interpenetration of lipids into the other leaflet of the bilayer may be another possible reason for the close hydrophilic/hydrophobic contact.
  • 60
    • 84906406741 scopus 로고    scopus 로고
    • 54 and cholesterol at 10 and 40% molar ratio; the relative fwhh is conserved by a factor of 3.
    • 54 and cholesterol at 10 and 40% molar ratio; the relative fwhh is conserved by a factor of 3.
  • 61
    • 84906363731 scopus 로고    scopus 로고
    • In the case of 1D transient NOE experiment, the temperature is set at 60°C, where Ch26-27 is narrower in order to resolve Ch26-27 and PC14
    • In the case of 1D transient NOE experiment, the temperature is set at 60°C, where Ch26-27 is narrower in order to resolve Ch26-27 and PC14.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.