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The extrusion was performed until the solution became transparent. At a higher cholesterol concentration, a larger number of passages were required for the solution to become transparent because of the cholesterol-induced rigidity of the bilayer membrane
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The extrusion was performed until the solution became transparent. At a higher cholesterol concentration, a larger number of passages were required for the solution to become transparent because of the cholesterol-induced rigidity of the bilayer membrane.
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It should be noted that here we show the NOE enhancement by (I(t m, I0]/I0, rather than Itm, I0, because the former represents the flux of intermolecular magnetization transfer between a specific pair of sites, whereas the latter, related to the cross relaxation rate, is proportional to the number of protons that receive magnetizations. The selective π pulse was applied for the choline group of PC. We do not explicitly take the intensity of the selectively inverted choline signal into account when we show the NOE enhancement, since the amount of PC was kept constant and the linewidth of the choline peak is not sensitive to the cholesterol content
-
0, because the former represents the flux of intermolecular magnetization transfer between a specific pair of sites, whereas the latter, related to the cross relaxation rate, is proportional to the number of protons that receive magnetizations. The selective π pulse was applied for the choline group of PC. We do not explicitly take the intensity of the selectively inverted choline signal into account when we show the NOE enhancement, since the amount of PC was kept constant and the linewidth of the choline peak is not sensitive to the cholesterol content.
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84906363736
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2O in pure solvent (ref 33).
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2O in pure solvent (ref 33).
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For the fwhh and chemical shift measurements at cholesterol concentrations of 1 mol%, a new probe for a sample tube of 20 mm diameter (JEOL ECA500W) was used.
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For the fwhh and chemical shift measurements at cholesterol concentrations of 1 mol%, a new probe for a sample tube of 20 mm diameter (JEOL ECA500W) was used.
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The interdigitation (for a review, see ref 46) or the interpenetration of lipids into the other leaflet of the bilayer may be another possible reason for the close hydrophilic/hydrophobic contact
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84906406741
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54 and cholesterol at 10 and 40% molar ratio; the relative fwhh is conserved by a factor of 3.
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54 and cholesterol at 10 and 40% molar ratio; the relative fwhh is conserved by a factor of 3.
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61
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84906363731
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In the case of 1D transient NOE experiment, the temperature is set at 60°C, where Ch26-27 is narrower in order to resolve Ch26-27 and PC14
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In the case of 1D transient NOE experiment, the temperature is set at 60°C, where Ch26-27 is narrower in order to resolve Ch26-27 and PC14.
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