Improved normalization of real-time reverse transcriptase polymerase chain reaction data using an external RNA control

Analytical Biochemistry

Volumn 376, Issue 1, 2008, Pages 83-93

  Ellefsen, Stian ^a   Stensløkken, Kåre Olav ^b   Sandvik, Guro K ^a   Kristensen, Tom A ^a   Nilsson, Göran E ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

External RNA control; HSC70; HSP30; Internal RNA control; Normalization; Real time RT PCR quantitative PCR; Reference gene

Indexed keywords

GENES; PROTEINS; RNA;

HSC70; HSP30; NORMALIZATION; REAL TIME RT-PCR; REFERENCE GENES;

POLYMERASE CHAIN REACTION;

BETA ACTIN; COMPLEMENTARY DNA; CYCLOPHILIN A; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HEAT SHOCK COGNATE PROTEIN 70; HEAT SHOCK PROTEIN 30; RNA;

ANIMAL TISSUE; ARTICLE; CARP; CONTROLLED STUDY; DNA SYNTHESIS; GENE; GENE EXPRESSION PROFILING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SEQUENCE ANALYSIS; STATISTICAL ANALYSIS; STRESS;

CARASSIUS; CARASSIUS CARASSIUS;

EID: 41449100793     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.01.028     Document Type: Article

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