Contruction of a chimeric thermostable pyrophosphatase to facilitate its purification and immobilization by using the choline-binding tag

Applied and Environmental Microbiology

Volumn 70, Issue 8, 2004, Pages 4642-4647

  Moldes, Cristina ^a,b   García, José L ^a   García, Pedro ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b IRFM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ASSAYS; ENZYME IMMOBILIZATION; ENZYMES; ESCHERICHIA COLI; HIGH TEMPERATURE EFFECTS;

CHOLINE; PYROPHOSPHATASE (PYR);

MICROBIOLOGY;

AUTOLYSIN; CELLULOSE; CHOLINE; CHOLINE DERIVATIVE; INORGANIC PYROPHOSPHATASE; LYT ANTIGEN; PROTEINASE; THROMBIN; HYBRID PROTEIN; IMMOBILIZED ENZYME; N ACETYLMURAMOYLALANINE AMIDASE;

MICROBIOLOGY;

ARTICLE; BIOTECHNOLOGY; CARBOXY TERMINAL SEQUENCE; CHIMERA; ENZYME PURIFICATION; ESCHERICHIA COLI; HIGH TEMPERATURE; NONHUMAN; PAPER; PROTEIN IMMOBILIZATION; SOLID; STREPTOCOCCUS PNEUMONIAE; THERMOSTABILITY; THERMUS THERMOPHILUS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HEAT; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; THERMUS;

ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS PNEUMONIAE; THERMUS; THERMUS THERMOPHILUS;

BASE SEQUENCE; BIOTECHNOLOGY; CHOLINE; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; HEAT; MOLECULAR SEQUENCE DATA; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; PYROPHOSPHATASES; RECOMBINANT FUSION PROTEINS; THERMUS;

EID: 4143138635     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.8.4642-4647.2004     Document Type: Article

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