Expression in Escherichia coli of the thermostable inorganic pyrophosphatase from the Aquifex aeolicus and purification and characterization of the recombinant enzyme

Protein Expression and Purification

Volumn 23, Issue 2, 2001, Pages 242-248

  Hoe, Hyang Sook ^a   Kim, Hyun Kyu ^a   Kwon, Suk Tae ^a  

^a Sungkyunkwan University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; COLUMN CHROMATOGRAPHY; DIVALENT CATION; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE OVEREXPRESSION; INORGANIC PYROPHOSPHATASE; MAGNESIUM ION; MOLECULAR WEIGHT; PH; PLASMID; POLYMERASE CHAIN REACTION; RECOMBINANT ENZYME; THERMOSTABILITY;

AQUIFEX AEOLICUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034756698     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1498     Document Type: Article

References (19)

1. On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions
2. Pyrophosphatase is essential for growth of Escherichia coli
3. Yeast PPA2 gene encodes a mitochondrial inorganic pyrophosphatase that is essential for mitochondrial function
4. Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12
5. Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae
6. Conservation of functional residues between yeast and Escherichia coli inorganic pyrophosphatase
7. Evolutionary conservation of the active site of soluble inorganic pyrophosphatase
8. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase: Effect of pyrophosphorysis and metal ions
9. Thermo Sequenase DNA polymerase and T. acidophilum pyrophosphatase: New thermostable enzyme for DNA sequencing
10. Molecular cloning, expression, and site-directed mutagenesis of inorganic pyrophosphatase from Thermus thermophilus HB27
11. Purification, cloning, and sequencing of archaebacterial pyrophosphatase from the extreme thermoacidophile Sulfolobus acidocaldarius
12. The complete genome of the hyperthermophilic bacterium Aquifex aeolicus
13. Cloning and analysis of DNA polymerase-encoding gene from Thermus caldophilus GK24
14. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase
15. Protein measurement with the Folin - Phenol reagent
16. Analytical gel chromatography of proteins
17. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel
18. Purification, properties, and multiple forms of a manganese-activated inorganic pyrophosphatase from Bacillus subtilis