Quantitative real-time RT-PCR measurement of cytokine mRNA expression in the skin of normal cats and cats with allergic skin disease

Veterinary Immunology and Immunopathology

Volumn 122, Issue 3-4, 2008, Pages 216-230

  Taglinger, K ^a   Van Nguyen, N ^b   Helps, C R ^b   Day, M J ^b   Foster, A P ^c  

^a University of Bristol ^*  (Austria)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c VETERINARY LABORATORIES AGENCY   (United Kingdom)

Author keywords

Feline; Real time RT PCR; Skin; Taq man probes

Indexed keywords

COMPLEMENTARY DNA; CYTOKINE; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 5; INTERLEUKIN 6; MESSENGER RNA; PROTEIN P35; PROTEIN P40; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL TISSUE; ARTICLE; CAT; EXON; FEMALE; GENE EXPRESSION; GENE PROBE; GENETIC TRANSCRIPTION; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; SKIN; SKIN ALLERGY; SKIN BIOPSY;

ANIMALS; CASE-CONTROL STUDIES; CAT DISEASES; CATS; CYTOKINES; DERMATITIS; FEMALE; GENE EXPRESSION REGULATION; MALE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SKIN;

FELIDAE;

EID: 40949138864     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetimm.2007.11.014     Document Type: Article

References (59)

1. Akdis C.A., Akdis M., Simon D., Dibbert B., Weber M., Gratzl S., Kreyden O., Disch R., Wuthrich B., Blaser K., and Simon H.U. T cells and T cell-derived cytokines as pathogenic factors in the nonallergic form of atopic dermatitis. J. Invest. Dermatol. 113 (1999) 628-634
2. Akdis M., Trautmann A., Blaser K., and Akdis C.A. T cells and effector mechanisms in the pathogenesis of atopic dermatitis. Curr. Allergy Asthma Rep. 2 (2002) 1-3
3. Arkwright P.D., Chase J.M., Babbage S., Pravica V., David T.J., and Hutchinson I.V. Atopic dermatitis is associated with a low-producer transforming growth factor beta(1) cytokine genotype. J. Allergy Clin. Immunol. 108 (2001) 281-284
4. Atkins M.B., Robertson M.J., Gordon M., Lotze M.T., DeCoste M., DuBois J.S., Ritz J., Sandler A.B., Edington H.D., Garzone P.D., Mier J.W., Canning C.M., Battiato L., Tahara H., and Sherman M.L. Phase I evaluation of intravenous recombinant human interleukin 12 in patients with advanced malignancies. Clin. Cancer Res. 3 (1997) 409-417
5. Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25 (2000) 169-193
6. Campbell D.J., Rawlings J.M., Koelsch S., Wallace J., Strain J.J., and Hannigan B.M. Age-related differences in parameters of feline immune status. Vet. Immunol. Immunopathol. 100 (2004) 73-80
7. Dinarello C.A. IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J. Allergy Clin. Immunol. 103 (1999) 11-24
8. Foster A.P. A study of the number and distribution of cutaneous mast cells in cats with diseases not affecting the skin. Vet. Dermatol. 5 (1994) 17-20
9. Gaffen S.L., and Liu K.D. Overview of interleukin-2 function, production and clinical applications. Cytokine 28 (2004) 109-123
10. Gately M.K., Desai B.B., Wolitzky A.G., Quinn P.M., Dwyer C.M., Podlaski F.J., Familletti P.C., Sinigaglia F., Chizonnite R., and Gubler U. Regulation of human lymphocyte proliferation by a heterodimeric cytokine, IL-12 (cytotoxic lymphocyte maturation factor). J. Immunol. 147 (1991) 874-882
11. Grewe M., Bruijnzeel-Koomen C.A., Schopf E., Thepen T., Langeveld-Wildschut A.G., Ruzicka T., and Krutmann J. A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis. Immunol. Today 19 (1998) 359-361
12. Hamid Q., Boguniewicz M., and Leung D.Y. Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis. J. Clin. Invest. 94 (1994) 870-876
13. Hamid Q., Naseer T., Minshall E.M., Song Y.L., Boguniewicz M., and Leung D.Y. In vivo expression of IL-12 and IL-13 in atopic dermatitis. J. Allergy Clin. Immunol. 98 (1996) 225-231
14. Heijink I.H., Vellenga E., Borger P., Postma D.S., de Monchy J.G., and Kaufman H.F. Interleukin-6 promotes the production of interleukin-4 and interleukin-5 by interleukin-2-dependent and -independent mechanisms in freshly isolated human T cells. Immunology 107 (2002) 316-324
15. Heufler C., Koch F., Stanzl U., Topar G., Wysocka M., Trinchieri G., Enk A., Steinman R.M., Romani N., and Schuler G. Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells. Eur. J. Immunol. 26 (1996) 659-668
16. Higashi N., Gesser B., Kawana S., and Thestrup-Pedersen K. Expression of IL-18 mRNA and secretion of IL-18 are reduced in monocytes from patients with atopic dermatitis. J. Allergy Clin. Immunol. 108 (2001) 607-614
17. Horsmanheimo L., Harvima I.T., Jarvikallio A., Harvima R.J., Naukkarinen A., and Horsmanheimo M. Mast cells are one major source of interleukin-4 in atopic dermatitis. Brit. J. Dermatol. 131 (1994) 348-353
18. Janeway C., Travers P., Walport M., and Shlomchik M. Immunobiology: The Immune System in Health and Disease (2001), Garland Publishing, New York
19. Jeong C.W., Ahn K.S., and Rho N.K. Differential in vivo cytokine mRNA expression in lesional skin of intrinsic vs, extrinsic atopic dermatitis patients using semiquantitative RT-PCR. Clin. Exp. Allergy 33 (2003) 1717-1724
20. Junghans V., Gutgesell C., Jung T., and Neumann C. Epidermal cytokines IL-1beta, TNF-alpha and IL-12 in patients with atopic dermatitis: response to applications of house dust mite antigens. J. Invest. Dermatol. 111 (1998) 1184-1188
21. Kawase Y., Hoshino T., Yokota K., Kuzuhara A., Kirii Y., Nishiwaki E., Maeda Y., Takeda J., Okamoto M., Kato S., Imaizumi T., Aizawa H., and Yoshino K. Exacerbated and prolonged allergic and non-allergic inflammatory cutaneous reaction in mice with targeted interleukin-18 expression in the skin. J. Invest. Dermatol. 121 (2003) 502-509
22. Kirman J., and Le Gros G. Which is the true regulator of TH2 cell development in allergic immune responses?. Clin. Exp. Allergy 28 (1998) 908-910
23. Koning H., Neijens H.J., Baert M.R., Oranje A.P., and Savelkoul H.F. T cell subsets and cytokines in allergic and non-allergic children. I. Analysis of IL-4, IFN-gamma and IL-13 mRNA expression and protein production. Cytokine 9 (1997) 416-426
24. Koning H., Neijens H.J., Baert M.R., Oranje A.P., and Savelkoul H.F. T cell subsets and cytokines in allergic and non-allergic children. II. Analysis and Il-5 and IL-10 mRNA expression and protein production. Cytokine 9 (1997) 427-436
25. Leung D.Y. Atopic dermatitis: new insights and opportunities for therapeutic intervention. J. Allergy Clin. Immunol. 105 (2000) 860-876
26. Leung D.Y., and Bieber T. Atopic dermatitis. Lancet 361 (2003) 151-160
27. Leung D.Y., Jain N., and Leo H.L. New concepts in the pathogenesis of atopic dermatitis. Curr. Opin. Immunol. 15 (2003) 634-638
28. Ling E., and Robinson D.S. Transforming growth factor-beta1: its anti-inflammatory and pro-fibrotic effects. Clin. Exp. Allergy 32 (2000) 175-178
29. Maeda S., Fujiwara S., and Omori K. Lesional expression of thymus and activation-regulated chemokine in canine atopic dermatitis. Vet. Immunol. Immunopathol. 88 (2002) 79-87
30. Mills K.H., and McGuirk P. Antigen-specific regulatory T cells-their induction and role in infection. Sem. Immunol. 16 (2004) 107-117
31. Moore K.W., de Waal Malefyt R., Coffman R.L., and O'Garra A. Interleukin-10 and the interleukin-10 receptor. Annu. Rev. Immunol. 19 (2001) 683-765
32. Nguyen Van N., Taglinger K., Helps C.R., Tasker S., Gruffydd-Jones T.J., and Day M.J. Measurement of cytokine mRNA expression in intestinal biopsies of cats with inflammatory enteropathy using quantitative real-time RT-PCR. Vet. Immunol. Immunopathol. 113 (2006) 404-414
33. Nomura I., Gao B., Boguniewicz M., Darst M.A., Travers J.B., and Leung D.Y. Distinct patterns of gene expression in the skin lesions of atopic dermatitis and psoriasis: a gene microarray analysis. J. Allergy Clin. Immunol. 112 (2003) 1195-1202
34. Nomura I., Goleva E., and Howell M.D. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J. Immunol. 171 (2003) 3262-3269
35. Nuttall T.J., Knight P.A., McAleese S.M., Lamb J.R., and Hill P.B. Expression of Th1, Th2 and immunosuppressive cytokine gene transcripts in canine atopic dermatitis. Clin. Exp. Allergy 32 (2002) 789-795
36. Nuttall T.J., Knight P.A., McAleese S.M., Lamb J.R., and Hill P.B. T-helper 1, T-helper 2 and immunosuppressive cytokines in canine atopic dermatitis. Vet. Immunol. Immunopathol. 87 (2002) 379-384
37. Ohmen J.D., Hanifin J.M., Nickoloff B.J., Rea T.H., Wyzykowaki R., Kim J., Jullien D., McHugh T., Nassif A.S., and Chan S.C. Overexpression of IL-10 in atopic dermatitis. Contrasting cytokine patterns with delayed-type hypersensitivity reactions. J. Immunol. 154 (1995) 1956-1963
38. Olivry T., Dean G.A., Tompkins M.B., Dow J.L., and Moore P.F. Toward a canine model of atopic dermatitis: amplification of cytokine-gene transcripts in the skin of atopic dogs. Exp. Dermatol. 8 (1999) 204-211
39. Overbergh L., Giulietti A., Valckx D., Decallonne R., Bouillon R., and Mathieu C. The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression. J. Biomol. Tech. 14 (2003) 33-43
40. Peters, I.R., 2003. A study of gastrointestinal IgA secretion in German Shepherd Dogs. PhD Thesis, University of Bristol.
41. Peters I.R., Helps C.R., Hall E.J., and Day M.J. Real-time RT-PCR: considerations for efficient and sensitive assay design. J. Immunol. Methods 286 (2004) 203-217
42. Plackett T.P., Schilling E.M., Faunce D.E., Choudry M.A., Witte P.L., and Kovacs E.J. Aging enhances lymphocyte cytokine defects after injury. FASEB J. 17 (2003) 688-689
43. Rincon M., Anguita J., Nakamura T., Fikrig E., and Flavell R.A. Interleukin (IL0-6 directs the differentiation of IL-4-producing CD4+ T cells. J. Exp. Med. 185 (1997) 461-469
44. Roosje P.J., Dean G.A., Willemse T., Rutten V., and Thepen T. Interleukin 4-producing CD4+ T cells in the skin of cats with allergic dermatitis. Vet. Pathol. 39 (2002) 228-233
45. Roosje P.J., Thepen T., Rutten V.P., Van Den Brom W.E., Bruijnzeel-Koomen C.A., and Willemse T. Immunophenotyping of the cutaneous cellular infiltrate after atopy patch testing in cats with atopic dermatitis. Vet. Immunol. Immunopathol. 101 (2004) 143-151
46. Roosje P.J., Koeman J.P., Thepen T., and Willemse T. Mast cells and eosinophils in feline allergic dermatitis: a qualitative and quantitative analysis. J. Comp. Pathol. 131 (2004) 61-69
47. Sanderson C.J. Interleukin-5, eosinophils and disease. Blood 79 (1992) 3101-3109
48. Scott D.W., Miller W.H., and Griffin C.E. Skin immune system and allergic diseases. Muller and Kirk's Small Animal Dermatology (2001), WB Saunders, Philadelphia pp. 543-666
49. Stoll S., Muller G., Kurimoto M., Saloga J., Tanimoto T., Yamauchi H., Okamura H., Knop J., and Enk A.H. Production of IL-18 (IFN-gamma-inducing factor) messenger RNA and functional protein by murine keratinocytes. J. Immunol. 159 (1997) 298-302
50. Sumiyoshi K., Nakao A., Ushio H., Mitsuishi K., Okumura K., Tsuboi R., Ra C., and Ogawa H. Transforming growth factor-beta1 suppresses atopic dermatitis-like skin lesions in NC/Nga mice. Clin. Exp. Allergy 32 (2002) 309-314
51. Tanaka Y., Delaporte E., Dubucquoi A.S., Porchet E., Capron A., and Capron M. Interleukin-5 messenger RNA and immunoreactive protein expression by activated eosinophils in lesional atopic dermatitis skin. J. Invest. Dermatol. 103 (1994) 589-592
52. Tanaka T., Tsutsui H., and Yoshimoto T. Interleukin-18 is elevated in the sera from patients with atopic dermatitis and from atopic dermatitis model mice, NC/Nga. Intern. Arch. Allergy Immunol. 125 (2001) 236-240
53. Tang M., Kemp A., and Varigos G. Il-4 and interferon-gamma production in children with atopic disease. Clin. Exp. Immunol. 92 (1993) 120-124
54. Tazawa T., Sugiura H., Sugiura Y., and Uehara M. relative importance of IL-4 and IL-13 in lesional skin of atopic dermatitis. Arch. Dermatol. Res. 295 (2004) 459-464
55. Thepen T., Langeveld-Wildschut E.G., Bihari I.C., van Wichen D.F., van Reijsen F.C., Mudde G.C., and Bruijnzeel-Koomen C.A. Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: an immunocytochemical study. J. Allergy Clin. Immunol. 97 (1996) 828-837
56. Van der Heijden F.L., Wierenga E.A., Bos J.D., and Kapsenberg M.L. High frequency of IL-4-producing CD4+ allergen-specific T lymphocytes in atopic dermatitis lesional skin. J. Invest. Dermatol. 97 (1991) 389-394
57. Van Reijsen F.C., Bruijnzeel-Koomen C.A., Kalthoff F.S., Maggi E., Romagnani S., Westland J.K., and Mudde G.C. Skin-derived aeroallergen-specific T-cell clones of Th2 phenotype in patients with atopic dermatitis. J. Allergy Clin. Immunol. 90 (1992) 184-193
58. Werfel T., Morita A., Grewe M., Renz H., Wahn U., Krutmann J., and Kapp A. Allergen specificity of skin-infiltrating T cells is not restricted to a type-2 cytokine pattern in chronic skin lesions of atopic dermatitis. J. Invest. Dermatol. 107 (1996) 871-876
59. Ying S., Durham S.R., Corrigan C.J., Hamid Q., and Kay A.B. Phenotype of cells expressing mRNA for TH2-type (interleukin 4 and interleukin 5) and TH1-type (interleukin 2 and interferon gamma) cytokines in bronchoalveolar lavage and bronchial biopsies from atopic asthmatic and normal control subjects. Am. J. Resp. Cell Mol. Biol. 12 (1995) 477-487