Specific ethanol production rate in ethanologenic Escherichia coli strain KO11 is limited by pyruvate decarboxylase

Journal of Molecular Microbiology and Biotechnology

Volumn 15, Issue 1, 2008, Pages 55-64

  Huerta Beristain, Gerardo ^a   Utrilla, José ^a   Hernández Chávez, Georgina ^a   Bolívar, Francisco ^a   Gosset, Guillermo ^a   Martinez, Alfredo ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

Escherichia coli; Fuel ethanol; Glycolytic flux; Metabolic control; Metabolic engineering; Phosphofructokinase; Pyruvate decarboxylase; Pyruvate kinase

Indexed keywords

6 PHOSPHOFRUCTOKINASE; ACETIC ACID; ADENOSINE TRIPHOSPHATE; ALCOHOL; CARBON; GALACTOSE; GLUCOSE; PYRUVATE DECARBOXYLASE; PYRUVATE KINASE; XYLOSE;

ALCOHOL PRODUCTION; ARTICLE; BACTERIAL STRAIN; CARBON METABOLISM; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; ZYMOMONAS MOBILIS;

CULTURE MEDIA; ESCHERICHIA COLI; ETHANOL; FERMENTATION; GLUCOSE; INDUSTRIAL MICROBIOLOGY; PHOSPHOFRUCTOKINASE-1; PYRUVATE DECARBOXYLASE; RECOMBINANT PROTEINS; XYLOSE;

ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; ZYMOMONAS MOBILIS;

EID: 40949137363     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000111993     Document Type: Article

References (41)

1. Aristidou A, Penttilä M: Metabolic engineering applications to renewable resource utilization. Curr Opin Biotechnol 2000;11:187-198.
2. Beall DS, Ohta K, Ingram LO: Parametric studies of ethanol production from xylose and other sugars by recombinant Escherichia coli. Biotechnol Bioeng 1991;38:296-303.
3. Böck A, Sawers G: Fermentation; in Neidhardt FC (ed): Escherichia coli and Salmonella. Cellular and Molecular Biology. Washington, American Society for Microbiology Press, 1996, vol 1, pp 262-282.
4. Conway T, Osman YA, Konnan JI, Hoffmann EM, Ingram LO: Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J Bacteriol 1987a;169:949-954.
5. Conway T, Sewell GW, Osman YA, Ingram LO: Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J Bacteriol 1987b;169:2591-2597.
6. Emmerling M, Bailey JE, Sauer U: Glucose catabolism of Escherichia coli strains with increased activity and altered regulation of key glycolytic enzymes. Metab Eng 1999;1:117-127.
7. Emmerling M, Bailey JE, Sauer U: Altered regulation of pyruvate kinase or co-overexpression of phosphofructokinase increases glycolytic fluxes in resting Escherichia coli. Biotechnol Bioeng 2000;67:623-627.
8. 13C labeling and NMR spectroscopy. Metab Eng 2002;4:124-137.
9. Garay-Arroyo A, Covarrubias AA, Clark I, Niño I, Gosset G. Martinez A: Response to different environmental stress conditions of industrial and laboratory Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol 2004;63:734-741.
10. Gonzalez R, Tao H, Purvis JE, York SW, Shanmugam KT, Ingram LO: Gene array-based identification of changes that contribute to ethanol tolerance in ethanologenic Escherichia coli: comparison of KO11 (Parent) to LY01 (Resistant Mutant). Biotechnol Prog 2003;19:612-623.
11. Gonzalez R, Tao H, Shanmugam KT, York SW, Ingram LO: Global gene expression differences associated with changes in glycolytic flux and growth rate in Escherichia coli during the fermentation of glucose and xylose. Biotechnol Prog 2002;18:6-20.
12. Hauf J, Zimmermann FK, Muller S: Simultaneous genomic overexpression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Enzyme Microbiol Technol 2000;26:688-698.
13. Hernández-Montalvo V, Martinez A, Hernández-Chávez G, Bolívar F, Valle F, Gosset G: Expression of galP and glk in a Escherichia coli PTS mutant restores glucose transport and increases glycolytic flux to fermentation products. Biotechnol Bioeng 2003;83:687-694.
14. Hernández-Montalvo V, Valle F, Bolívar F, Gosset G: Characterization of sugar mixtures utilization by an Escherichia coli mutant devoid of the phosphotransferase system. Appl Microbiol Biotechnol 2001;57:186-191.
15. Himmel ME, Adney WS, Baker JO, Elander R, McMillan JD, Nieves RA, Sheehan JJ, Thomas SR, Vinzant TB, Zhang M: Advanced bioethanol production technologies: a perspective. ACS Symp Ser 1997;666:2-45.
16. Hoppner TC, Doelle HW: Purification and kinetic characteristics of pyruvate decarboxylase and ethanol dehydrogenase from Zymomonas mobilis in relation to ethanol production. Eur J Appl Microbiol Biotechnol 1983;17:152-157.
17. Ingram LO, Gomez PF, Lai X, Moniruzzaman M, Wood BE, Yomano LP, York SW: Metabolic engineering of bacteria for ethanol production. Biotechnol Bioeng 1998;58:204-214.
18. Ingram LO, Aldrich HC, Borges ACC, Causey TB, Martinez A, Morales F, Saleh A, Underwood SA, Yomano LP, York SW, Zaldivar J, Zhou S: Enteric bacterial catalyst for fuel ethanol production. Biotechnol Prog 1999;15:855-866.
19. Koebmann BJ, Andersen HW, Solem C, Jensen PR: Experimental determination of control of glycolysis in Lactococcus lactis. Antonie van Leeuwenhoek 2002a;82:237-248.
20. Koebmann BJ, Westerhoff HV, Snoep JL, Nilsson D, Jensen PR: The glycolytic flux in Escherichia coli is controlled by the demand for ATP. J Bacteriol 2002b;184:3909-3916.
21. Lagunas R, Dominguez C, Busturia A, Saez MJ: Mechanisms of appearance of the Pasteur effect in Saccharomyces cerevisiae: inactivation of sugar transport systems. J Bacteriol 1982;152:19-25.
22. Larsson C, Pahlman I-L, Gustafsson L: The importance of ATP as a regulator of glycolytic flux in Saccharomyces cerevisiae. Yeast 2000;16:797-809.
23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-275.
24. Maitra PK, Lobo Z: A kinetic study of glycolytic enzyme synthesis in yeast. J Biol Chem 1971;246:475-488.
25. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 1982.
26. Martinez A, Rodriguez ME, Wells ML, York SW, Preston JF, Ingram LO: Detoxification of dilute acid hydrolysates of lignocellulose with lime. Biotechnol Prog 2001;17:287-293.
27. Martinez A, York SW, Yomano LP, Pineda VL, Davis FC, Shelton JC, Ingram LO: Biosynthetic burden and plasmid burden limit expression of chromosomally integrated heterologous genes (pdc, adhB) in Escherichia coli. Biotechnol Prog 1999;15:891-897.
28. Nelleman LJ, Holm F, Atlung T, Hansen FG: Cloning and characterization of the Escherichia coli phosphoglycerate kinase (pgk) gene. Gene 1989;77:185-191.
29. Ohta K, Alterthum F, Ingram LO: Effects of environmental conditions on xylose fermentation by recombinant Escherichia coli. Appl Environ Microbiol 1990;56:463-465.
30. Ohta K, Beall DS, Shanmugam KT, Ingram LO: Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl Environ Microbiol 1991;57:893-900.
31. Rogers PL, Lee KJ, Tribe DE: Kinetics of alcohol production by Zymomonas mobilis at high sugar concentrations. Biotechnol Lett 1979;1:165-170.
32. Sakai H, Susuki K, Imahori K: Purification and properties of pyruvate kinase from Bacillus stearothermophilus. J Biochem 1986;99:1157-1167.
33. Schaaff I, Heinisch J, Zimmermann FK: Overproduction of glycolytic enzymes in yeast. Yeast 1989;5:285-290.
34. Snoep JL, Yomano LP, Westerhoff HV, Ingram LO: Protein burden in Zymomonas mobilis: negative flux and growth control due to overproduction of glycolytic enzymes. Microbiology 1995;141:2329-2337.
35. Stephanopoulos G: Metabolic fluxes and metabolic engineering. Metab Eng 1999;1:1-11.
36. Tao H, Gonzalez R, Martinez A, Rodriguez M, Ingram LO, Preston JF, Shanmugam KT: Engineering a homo-ethanol pathway in Escherichia coli: increased glycolytic flux and levels of expression of glycolytic genes during xylose fermentation. J Bacteriol 2001;183:2979-2988.
37. Tarmy EM, Kaplan NO: Kinetics of Escherichia coli B D-Lactate dehydrogenase and evidence for pyruvate-controlled change in conformation. J Biol Chem 1968;243:2587-2596.
38. Underwood SA, Buszko ML, Shanmugam KT, Ingram LO: Flux through citrate synthase limits the growth of ethanologenic Escherichia coli KO11 during xylose fermentation. Appl Environ Microbiol. 2002a;68:1071-1081.
39. Underwood SA, Zhou S, Causey TB, Yomano LP, Shanmugam KT, Ingram LO: Genetic changes to optimize carbon partitioning between ethanol and biosynthesis in ethanologenic Escherichia coli. Appl Environ Microbiol 2002b;68:6263-6272.
40. Vellanoweth RL, Rabinowitz JC: The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Mol Microbiol 1992;6:1105-1114.
41. Waygood EB, Sanwal BD: The control of pyruvate kinases of Escherichia coli. I. Physicochemical and regulatory properties of the enzymes activated by fructose 1,6-diphosphate. J Biol Chem 1974;249:265-274.