Biosynthetic burden and plasmid burden limit expression of chromosomally integrated heterologous genes (pdc, adhB) in Escherichia coli

Biotechnology Progress

Volumn 15, Issue 5, 1999, Pages 891-897

  Martinez, Alfredo ^a,b   York, Sean W ^a   Yomano, Lorraine P ^a   Van Pineda, L ^a   Davis, Francis G ^a   Shelton, John C ^a   Ingram, Lonnie O ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; BIOSYNTHESIS; GENE EXPRESSION; GENE TRANSFER; PLASMID;

BIOCATALYSTS; BIOCONVERSION; BIOSYNTHESIS; DNA; ENZYMES; ESCHERICHIA COLI; ETHANOL; FERMENTATION; PRODUCTION ENGINEERING;

ETHANOLOGENIC RECOMBINANTS; PYRUVATE DECARBOXYLASE; ZYMOMONAS MOBILIS;

GENES;

CHROMOSOMES, BACTERIAL; CULTURE MEDIA; ESCHERICHIA COLI; ETHANOL; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, BACTERIAL; GENETIC ENGINEERING; PLASMIDS; PYRUVATE DECARBOXYLASE; ZYMOMONAS;

EID: 0033199419     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp990103p     Document Type: Article

References (37)

1. Alterthum, F.; Ingram, L. O. Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli. Appl. Environ. Microbiol. 1989, 55, 1943-1948.
2. Asghari, A.; Bothast, R. J.; Doran, J. B.; Ingram, L. O. Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineered Escherichia coli strain KO11. J. Ind. Microbiol. 1996, 16, 42-47.
3. Atlas, R. M., Parks, L. C., Eds. Handbook of Microbiological Media; CRC Press: Boca Raton, FL, 1993.
4. Beall, D. S.; Ohta, K.; Ingram; L. O. Parametric studies of ethanol production from xylose and other sugars by recombinant Escherichia coli. Biotechnol. Bioeng. 1991, 38, 296-303.
5. Benson, D. A.; Boguski, M. S.; Lipman, D. J.; Ostell, J.; Ouellette, B. F. GenBank. Nucleic Acids Res. 1998, 26, 1-7.
6. Conway, T.; Osman, Y. A.; Konnan, J. L; Hoffmann, E. M.; Ingram, L. O. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 1987, 169, 949-954.
7. Conway, T.; Sewell, G. W.; Osman, Y. A.; Ingram, L. O. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 1987, 169, 2591-2597.
8. Devereux, J.; Haeberli, P.; Smithies, O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984, 12, 387-395.
9. Drapal, N.; Sawers, G. Promoter 7 of the Escherichia coli pfl operon is a major determinant in the anaerobic regulation of expression by ArcA. J. Bacteriol. 1995, 177, 5338-5341.
10. Helmann, J. D.; Chamberlin, M. J. Structure and function of bacterial sigma factors. Annu. Rev. Biochem. 1988, 57, 839-872.
11. Holms, H. Flux analysis and control of the central metabolic pathways in Escherichia coli. FEMS Microbiol. Rev. 1996, 19, 85-116.
12. Hoppner, T. C.; Doelle, H. W. Purification and kinetic characteristics of pyruvate decarboxylase and ethanol dehydrogenase from Zymomonas mobilis in relation to ethanol production. Eur. J. Appl. Microbiol. Biotechnol. 1983, 17, 152-157.
13. Ingram, L. O.; Conway, T. Expression of different levels of ethanologenic enzymes of Zymomonas mobilis in recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 1988, 54, 397-404.
14. Ingram, L. O.; Gomez, P. F.; Lai, X.; Moniruzzaman, M.; Wood, B. E.; Yomano L. P.; York, S. W. Metabolic engineering of bacteria for ethanol production. Biotechnol. Bioeng. 1998, 58, 204-214.
15. Jensen, K. F.; Pedersen, S. Metabolic growth rate control in Escherichia coli may be a consequence of subsaturation of the macromolecular biosynthetic apparatus with substrates and catalytic components. Microbiol. Rev. 1990, 54, 89-100.
16. Jishage, M.; Iwata, A.; Ueda, S.; Ishihama, A. Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: Intracellular levels of four species of sigma subunit under various growth conditions. J. Bacteriol. 1996, 178, 5447-5451.
17. Lai, X.; Davis, F. C.; Hespell, R. B.; Ingram, L. O. Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Appl. Environ. Microbiol. 1997, 63, 355-363.
18. Lawford, H. G.; Rousseau, J. D. Studies on nutrient requirements and cost-effective supplements for ethanol production by recombinant E. coli. Appl. Biochem. Biotechnol. 1996, 57/58, 307-326.
19. Lenski, R. E.; Souza, V.; Doung, L. P.; Plan, Q. G.; Nguyen, T. N. M.; Bertrand, K. P. Epistatic effects of promoter and represser functions of the Tn10 tetracycline-resistance operon on the fitness of Escherichia coli. Mol. Ecol. 1994, 3, 127-135.
20. Mackenzie, K. F.; Eddy, C. K.; Ingram, L. O. Modulation of alcohol dehydrogenase isoenzyme levels in Zymomonas mobilis by iron and zinc. J. Bacteriol. 1989, 171, 1063-1067.
21. Makrides, S. C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 1996, 60, 512-538.
22. Marr, A. G. Growth rate of Escherichia coli. Microbiol. Rev. 1991, 55, 316-333.
23. Neale, A. D.; Scopes, R. K.; Kelly, J. M.; Wettenhall, R. E. H. The two alcohol dehydrogenases of Zymomonas mobilis. Purification by differential dye ligand chromatography, molecular characterisation and physiological roles. Eur. J. Biochem. 1986, 154, 119-124.
24. Ohta, K.; Alterthum, F.; Ingram; L. O. Effects of environmental conditions on xylose fermentation by recombinant Escherichia coli. Appl. Environ. Microbiol. 1990, 56, 463-465.
25. Ohta, K.; Beall, D. S.; Shanmugam, K. T.; Ingram; L. O. Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl. Environ. Microbiol. 1991, 57, 893-900.
26. Rasmussen, L. J.; Moeller, P. L.; Atlung, T. Carbon metabolism regulates expression of the pfl (pyruvate-formatelyase) gene in Escherichia coli. J. Bacteriol. 1991, 173, 6390-6397.
27. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular cloning: a laboratory manual, 2nd ed; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
28. Satyagal, V. N.; Agrawal, P. Cellular plasmid content and cloned-gene expression: some useful equations. Biotechnol. Bioeng. 1990, 35, 23-30.
29. 70-dependent transcription in vitro and heterologous expression in Pseudomonas putida in vivo. J. Bacteriol. 1989, 171, 4930-4937.
30. Sirko, A; Zehelein, E.; Freundlich, M.; Sawers, G. Integration host factor is required for anaerobic pyruvate induction of pfl operon expression in Escherichia coli. J. Bacteriol. 1993, 175, 5769-5777.
31. Snoep, J. L.; Yomano, L. P.; Westerhoff, H. V.; Ingram, L. O. Protein burden in Zymomonas mobilis: Negative flux and growth control due to overproduction of glycolytic enzymes. Microbiology 1995, 141, 2329-2337.
32. Togna, A. P.; Shuler, M. L.; Wilson, D. B. Effects of plasmid copy number and runaway plasmid replication on overproduction and excretion of beta-lactamase from Escherichia coli. Biotechnol. Prog. 1993, 9, 31-39.
33. 8. J. Bacteriol. 1996, 178, 2785-2793.
34. Yomano, L. P.; Scopes, R. K.; Ingram, L. O. Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli. J. Bacteriol. 1993, 175, 3926-3933.
35. Yomano, L. P.; York, S. W.; Ingram, L. O. Isolation and characterization of ethanol tolerant mutants of Escherichia coli KO11 for fuel ethanol production. J. Ind. Microbiol. 1998, 20, 132-138.
36. York, S. W.; Ingram, L. O. Ethanol production by recombinant Escherichia coli KO11 using crude yeast autolysate as a nutrient supplement. Biotechnol. Lett. 1996, 18, 683-688.
37. York, S. W.; Ingram, L. O. Soy-based medium for ethanol production by Escherichia coli KO11. J. Ind. Microbiol. 1996, 16, 374-376.