Analysis of carbon metabolism in Escherichia coli strains with an inactive phosphotransferase system by 13C labeling and NMR spectroscopy

Metabolic Engineering

Volumn 4, Issue 2, 2002, Pages 124-137

  Flores, S ^a   Gosset, G ^a   Flores, N ^a   De Graaf, A A ^b   Bolivar F ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Central carbon metabolism; Glucose transport; Metabolic engineering; NMR; Phosphoenolpyruvate

Indexed keywords

ESCHERICHIA COLI;

EID: 0036526245     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.2001.0209     Document Type: Article

References (36)

1. Determination of 3-deoxy-D-arabino-heptulosonate 7-phosphate productivity and yield from glucose in Escherichia coli devoid of the glucose phosphotransferase transport system
2. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system
3. Tricarboxylic acid cycle and glyoxylate bypass
4. Pathways of NADPH formation in Escherichia coli
5. Phosphorylation of d-glucose in Escherichia coli mutants defective in glucose phosphotransferase, mannose phosphotransferase, and glucokinase
6. From a soup or a seed? Pyritic metabolic complexes in the origin of life
7. 13C isotopomer distributions for metabolic flux analysis using heteronuclear spin echo difference NMR spectroscopy
8. 13C NMR flux ratio analysis of Escherichia coli central carbon metabolism in microaerobic bioprocesses
9. Pathway engineering for the production of aromatic compounds in Escherichia coli
10. Central metabolism
11. A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli
12. Multiple forms of Pseudomonas multivorans glucose-6-phosphate and 6-phosphogluconate dehydrogenases: Differences in size, pyridine nucleotide specificity, and susceptibility to inhibition by adenosine 5′-triphosphate
13. A kinetic study of glycolytic enzyme synthesis in yeast
14. + symport protein (GalP) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar
15. Why and when channelling can decrease pool size at constant net flux in a simple dynamic channel
16. Bidirectional reaction steps in metabolic networks. Part IV. Optimal design of isotopomer labeling experiments
17. Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli
18. Biosynthesis and fueling
19. Pathway engineering for production of aromatics in Escherichia coli: Confirmation of stoichiometric analysis by independent modulation of AroG, Tkt, and Pps activities
20. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum
21. Cloning of two pyruvate kinase isoenzymes structural genes from Escherichia coli: The relative role of these enzymes in pyruvate biosynthesis
22. Phosphoenolpyruvate: Carbohydrate phosphotransferase system
23. Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism
24. Detection of elementary flux nodes in biochemical networks: A promising tool for pathway analysis and metabolic engineering
25. Kinetic characterization in batch continuous culture of Escherichia coli mutants affected in phosphoenolpyruvate metabolism: Differences in acetic acid production
26. Substrate channeling
27. +-dependent malic enzyme in an Escherichia coli mutant
28. 13C-labeling of proteinogenic amino acids - An efficient analytical tool to investigate intermediary metabolism
29. 13C-NMR, MS and metabolic flux balancing in biotechnology research
30. Basic and applied aspects of metabolic diversity: The phosphoenolpyruvate node
31. Functions of the membrane-associated and cytoplasmic malate dehydrogenases in the citric acid cycle of Escherichia coli
32. Biochemical production capabilities of Escherichia coli
33. Alternative binding of two sequential glycolytic enzymes to microtubules: Molecular studies in phosphofructokinase/aldolase/microtubule system
34. Bidirectional reaction steps in metabolic networks. Part III. Explicit solution and analysis of isotopomer labelling systems