High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains - Selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG

Glycoconjugate Journal

Volumn 25, Issue 3, 2008, Pages 245-257

  Blanchard, Véronique ^a,c   Gadkari, Rupali A ^b   George, Albert V E ^a   Roy, Satarupa ^b   Gerwig, Gerrit J ^a   Leeflang, Bas R ^a   Dighe, Rajan R ^b   Boelens, Rolf ^a   Kamerling, Johannis P ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b INDIAN INSTITUTE OF SCIENCE   (India)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

15N Labeling; Glycosylation; Human chorionic gonadotropin; Human follicle stimulating hormone; Hypermannosylation; Pichia pastoris GS115; Pichia pastoris X 33

Indexed keywords

AMMONIA; CARBON; CHORIONIC GONADOTROPIN; FOLLITROPIN; GLYCEROL; GLYCOPROTEIN; MANNOSE; METHANOL; NITROGEN;

ANIMAL CELL; ARTICLE; CONFORMATION; CONTROLLED STUDY; FUNGAL STRAIN; GLYCOSYLATION; HORMONE RELEASE; MOUSE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PICHIA PASTORIS; PRIORITY JOURNAL; RADIOIMMUNOASSAY; RADIORECEPTOR ASSAY;

ANIMALS; CELLS, CULTURED; CHORIONIC GONADOTROPIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; GENE EXPRESSION; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MICE; PICHIA; POLYSACCHARIDES; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

PICHIA PASTORIS;

EID: 40949094021     PISSN: 02820080     EISSN: 15734986     Source Type: Journal    
DOI: 10.1007/s10719-007-9082-8     Document Type: Article

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