An optimized lentivirus-mediated RNAi screen reveals kinase modulators of kinesin-5 inhibitor sensitivity

Assay and Drug Development Technologies

Volumn 6, Issue 1, 2008, Pages 105-119

  Klinghoffer, Richard A ^a   Roberts, Brian ^a   Annis, James ^a   Frazier, Jason ^a   Lewis, Patrick ^a   Linsley, Peter S ^a   Cleary, Michele A ^a  

^a ROSETTA INPHARMATICS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AURORA A KINASE; CYCLIN DEPENDENT KINASE 1; ENZYME INHIBITOR; KINESIN 5 INHIBITOR; LENTIVIRUS VECTOR; PHOSPHOTRANSFERASE; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ARTICLE; AUTOMATION; CELL DIVISION; CELL GROWTH; CELL LINE; CONTROLLED STUDY; DRUG SENSITIVITY; GENE SILENCING; GENE TARGETING; GENETIC SCREENING; GROWTH INHIBITION; HUMAN; HUMAN CELL; MITOSIS SPINDLE; RNA INTERFERENCE;

AUTOMATION; CELL CYCLE; CELL LINE; CELL SURVIVAL; DRUG EVALUATION, PRECLINICAL; GENE SILENCING; GENETIC VECTORS; HELA CELLS; HT29 CELLS; HUMANS; KINESIN; LENTIVIRUS; LENTIVIRUS INFECTIONS; MICROARRAY ANALYSIS; NUCLEIC ACID CONFORMATION; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, VIRAL; ROBOTICS; TRANSFECTION;

EID: 40749091246     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2007.106     Document Type: Article

References (41)

1. Pelkmans L, Fava E, Grabner H, Hannus M, Habermann B, Krausz E, et al.: Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis. Nature 2005;436:78-86.
2. Llanos S, Efeyan A, Monsech J, Dominguez O, Serrano M: A high-throughput loss-of-function screening identifies novel p53 regulators. Cell Cycle 2006;5:1880-1885.
3. Bartz SR, Zhang Z, Burchard J, Imakura M, Martin M, Palmieri A, et al.: Small interfering RNA screens reveal enhanced cisplatin cytotoxicity in tumor cells having both BRCA network and TP53 disruptions. Mol Cell Biol 2006;26:9377-9386.
4. Kittler R, Putz G, Pelletier L, Poser I, Heninger AK, Drechsel D, et al.: An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 2004;432:1036-1040.
5. Abbas-Terki T, Blanco-Bose W, Deglon N, Pralong W, Aebischer P: Lentiviral-mediated RNA interference. Hum Gene Ther 2002;13:2197-2201.
6. Brummelkamp TR, Bernards R, Agami R: Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell 2002;2:243-247.
7. Paddison PJ, Caudy AA, Hannon GJ: Stable suppression of gene expression by RNAi in mammalian cells. Proc Natl Acad Sci U S A 2002;99:1443-1448.
8. Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, et al.: Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA 2003;9:493-501.
9. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, et al.: A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 2006;124:1283-1298.
10. Vale RD, Fletterick RJ: The design plan of kinesin motors. Annu Rev Cell Dev Biol 1997;13:745-777.
11. Dagenbach EM, Endow SA: A new kinesin tree. J Cell Sci 2004;117:3-7.
12. Enos AP, Morris NR: Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans. Cell 1990;60:1019-1027.
13. Blangy A, Lane HA, d'Herin P, Harper M, Kress M, Nigg EA: Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 1995;83:1159-1169.
14. Sawin KE, Mitchison TJ: Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. Proc Natl Acad Sci U S A 1995;92:4289-4293.
15. Tao W, South VJ, Zhang Y, Davide JP, Farrell L, Kohl NE, et al.: Induction of apoptosis by an inhibitor of the mitotic kinesin KSP requires both activation of the spindle assembly checkpoint and mitotic slippage. Cancer Cell 2005;8:49-59.
16. Mayer TU, Kapoor TM, Haggarty SJ, King RW, Schreiber SL, Mitchison TJ: Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science 1999;286:971-974.
17. Kapoor TM, Mayer TU, Coughlin ML, Mitchison TJ: Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J Cell Biol 2000;150:975-988.
18. Sakowicz R, Finer JT, Beraud C, Crompton A, Lewis E, Fritsch A, et al.: Antitumor activity of a kinesin inhibitor. Cancer Res 2004;64:3276-3280.
19. Rowinsky EK, Chaudhry V, Cornblath DR, Donehower RC: Neurotoxicity of taxol. J Natl Cancer Inst Monogr 1993;107-115.
20. Higashikawa F, Chang L: Kinetic analyses of stability of simple and complex retroviral vectors. Virology 2001;280:124-131.
21. Naldini L, Blomer U, Gage FH, Trono D, Verma IM: Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci U S A 1996;93:11382-11388.
22. Zufferey R, Nagy D, Mandel RJ, Naldini L, Trono D: Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat Biotechnol 1997;15:871-875.
23. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, et al.: A third-generation lentivirus vector with a conditional packaging system. J Virol 1998;72:8463-8471.
24. Butler SL, Hansen MS, Bushman FD: A quantitative assay for HIV DNA integration in vivo. Nat Med 2001;7:631-634.
25. Jackson AL, Bartz SR, Schelter J, Kobayashi SV, Burchard J, Mao M, et al.: Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol 2003;21:635-637.
26. van 't Veer LJ, Dai H, van de Vijver MJ, He YD, Hart AA, Mao M, et al.: Gene expression profiling predicts clinical outcome of breast cancer. Nature 2002;415:530-536.
27. Logan AC, Nightingale SJ, Haas DL, Cho GJ, Pepper KA, Kohn DB: Factors influencing the titer and infectivity of lentiviral vectors. Hum Gene Ther 2004;15:976-988.
28. Gasmi M, Glynn J, Jin MJ, Jolly DJ, Yee JK, Chen ST: Requirements for efficient production and transduction of human immunodeficiency virus type 1-based vectors. J Virol 1999;73:1828-1834.
29. Sakoda T, Kasahara N, Hamamori Y, Kedes L: A high-titer lentiviral production system mediates efficient transduction of differentiated cells including beating cardiac myocytes. J Mol Cell Cardiol 1999;31:2037-2047.
30. Karolewski BA, Watson DJ, Parente MK, Wolfe JH: Comparison of transfection conditions for a lentivirus vector produced in large volumes. Hum Gene Ther 2003;14:1287-1296.
31. Gray D, Jubb AM, Hogue D, Dowd P, Kljavin N, Yi S, et al.: Maternal embryonic leucine zipper kinase/murine protein serine-threonine kinase 38 is a promising therapeutic target for multiple cancers. Cancer Res 2005;65:9751-9761.
32. Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, Castle J, et al.: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 2005;433:769-773.
33. Jackson AL, Burchard J, Schelter J, Chau BN, Cleary M, Lim L, et al.: Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity. RNA 2006;12:1179-1187.
34. Vassilev LT, Tovar C, Chen S, Knezevic D, Zhao X, Sun H, et al.: Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc Natl Acad Sci U S A 2006;103:10660-10665.
35. Bridge AJ, Pebernard S, Ducraux A, Nicoulaz AL, Iggo R: Induction of an interferon response by RNAi vectors in mammalian cells. Nat Genet 2003;34:263-264.
36. Klampfer L, Huang J, Corner G, Mariadason J, Arango D, Sasazuki T, et al.: Oncogenic Ki-ras inhibits the expression of interferon-responsive genes through inhibition of STAT1 and STAT2 expression. J Biol Chem 2003;278:46278-46287.
37. Pebernard S, Iggo RD: Determinants of interferon-stimulated gene induction by RNAi vectors. Differentiation 2004;72:103-111.
38. Fish RJ, Kruithof EK: Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. BMC Mol Biol 2004;5:9.
39. Stojdl DF, Lichty B, Knowles S, Marius R, Atkins H, Sonenberg N, et al.: Exploiting tumor-specific defects in the interferon pathway with a previously unknown oncolytic virus. Nat Med 2000;6:821-825.
40. MacKeigan JP, Murphy LO, Blenis J: Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance. Nat Cell Biol 2005;7:591-600.
41. O'Connor DS, Wall NR, Porter AC, Altieri DC: A p34(cdc2) survival checkpoint in cancer. Cancer Cell 2002;2:43-54.