Protein transduction of an antioxidant enzyme: Subcellular localization of superoxide dismutase fusion protein in cells

Journal of Biochemistry and Molecular Biology

Volumn 41, Issue 2, 2008, Pages 170-175

  Dae, Won Kim ^a   So, Young Kim ^a   Sun, Hwa Lee ^a   Yeum, Pyo Lee ^a   Min, Jung Lee ^a   Min, Seop Jeong ^a   Sang, Ho Jang ^a   Park, Jinseu ^a   Kil, Soo Lee ^a   Kang, Tae Cheon ^a   Moo, Ho Won ^a   Cho, Sung Woo ^b   Kwon, Oh Shin ^c   Won, Sik Eum ^a   Soo, Young Choi ^a  

^a Hallym University   (South Korea)

^b University of Ulsan College of Medicine   (South Korea)

^c Kyungpook National University   (South Korea)

Author keywords

MTS; NLS; Protein therapy; Protein transduction; Subcellular localization

Indexed keywords

ANTIOXIDANT; HERBICIDE; HYBRID PROTEIN; PARAQUAT; PEPTIDE FRAGMENT; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE;

ARTICLE; CELL FRACTIONATION; CELL NUCLEUS; CELL SURVIVAL; CYTOSOL; DRUG EFFECT; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC TRANSDUCTION; GENETICS; HELA CELL; HUMAN; INTRACELLULAR MEMBRANE; ISOLATION AND PURIFICATION; METABOLISM; NUCLEAR LOCALIZATION SIGNAL; OXIDATIVE STRESS; WESTERN BLOTTING;

ANTIOXIDANTS; BLOTTING, WESTERN; CELL NUCLEUS; CELL SURVIVAL; CYTOSOL; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELLS; HERBICIDES; HUMANS; INTRACELLULAR MEMBRANES; NUCLEAR LOCALIZATION SIGNALS; OXIDATIVE STRESS; PARAQUAT; PEPTIDE FRAGMENTS; REACTIVE OXYGEN SPECIES; RECOMBINANT FUSION PROTEINS; SUBCELLULAR FRACTIONS; SUPEROXIDE DISMUTASE; TRANSDUCTION, GENETIC;

MAMMALIA;

EID: 40549119796     PISSN: 12258687     EISSN: 02191024     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Egleton, R. D. and Davis, T. P. (1997) Bioavailability and transport of peptides and peptide drugs into the brain. Peptides 18, 1431-1439.
2. Caron, N. J., Torrente, Y., Camirand, G., Bujold, M., Chapdelaine, P., Leriche, K., Bresolin, N. and Tremblay, J. P. (2001) Intracellular delivery of a Tat-eGFP fusion protein into muscle cells. Mol. Ther. 3, 310-318.
3. Ezhevsky, S. A., Ho, A., Becker-Hapak, M., Davis, P. K. and Dowdy, S. F. (2001) Differential regulation of retinoblastoma tumor suppressor protein by G(1) cyclin-dependent kinase complexes in vivo. Mol. Cell. Biol. 21, 4773-4783.
4. Kim, D., Jeon, C., Kim, J. H., Kim, M. S., Yoon, C. H., Choi, I. S., Kim, S. H. and Bae, Y. S. (2006) Cytoplasmic transduction peptide (CTP): new approach for the delivery of biomolecules into cytoplasm in vitro and in vivo. Exp. Cell Res. 312, 1277-1288.
5. 2 cytoplastomic domain. Am. J. Pathol. 159, 983-992.
6. Kwon, H. Y., Eum, W. S., Jang, H. W., Kang, J. H., Ryu, J. Y., Lee, B. R., Jin, L. H., Park, J. and Choi, S. Y. (2000) Transduction of Cu,Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into mammalian cells. FEBS Lett. 485, 163-167.
7. Kwon, J. H., Kim, J. B., Lee, K. H., Kang, S. M., Chung, N., Jang, Y. and Chung, J. H. (2007) Protective effect of heat shock protein 27 using protein transduction domain-mediated delivery on ischemia/reperfusion heart injury. Biochem. Biophys. Res. Commun. 363, 399-404.
8. Morris, M. C., Depollier, J., Mery, J., Heitz, F. and Divita, G. (2001) A peptide carrier for the delivery of biologically active proteins into mammalian cells. Nat. Biotechnol. 19, 1173-1176.
9. kip1 induces cell migration. Nat. Med. 4, 1449-1452.
10. Schwarze, S. R., Ho, A., Vocero-Akbani, A. and Dowdy, S. R. (1999) In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 285, 1569-1572.
11. Wadia, J. and Dowdy, S. F. (2002) Protein transduction technology. Curr. Opin. Biotechnol. 13, 52-56.
12. Wills, K. N., Atencio, I. A., Avanzini, J. B., Neuteboom, S., Phelan, A., Philopena, J., Sutjipto, S., Valilancourt, M. T., Wen, S. F., Ralston, R. O. and Johnson, D. E. (2001) Intratumoral spread and increased efficacy of a p53-VP22 fusion protein expressed by a recombinant adenovirus. J. Virol. 75, 8733-8741.
13. Fawell, S., Seery, J. and Daikh, Y. (1991) Tat-mediated delivery of heterologous proteins into cells. Proc. Natl. Acad. Sci. USA 91, 664-668.
14. Prochiantz, A. (2000) Messenger proteins: homeoproteins, TAT and others. Curr. Opin. Cell Biol. 12, 400-406.
15. Vives, E., Brodin, P. and Lebleu, B. (1997) A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J. Biol. Chem. 272, 16010-16017.
16. Eum, W. S., Choung, I. S., Li, M. Z., Kang, J. H., Kim, D. W., Park, J., Kwon, H. Y. and Choi, S. Y. (2004) HIV-1 Tat mediated protein transduction of Cu,Zn-superoxide dismutase into pancreatic β cells in vitro and in vivo. Free Radic. Biol. Med. 37, 339-349.
17. Choi, H. S., An, J. J., Kim, S. Y., Lee, S. H., Kim, D. W., Yoo, K. Y., Won, M. H., Kang, T. C., Kwon, H. J., Kang, J. H., Cho, S. W., Kwon, O. S., Park, J., Eum, W. S. and Choi, S. Y. (2006) PEP-1-SOD fusion protein efficiently protects against paraquat-induced dopaminergic neuron damage in a Parkinson disease mouse model. Free Radic. Biol. Med. 41, 1058-1068.
18. Ferrari, A., Pellegrini, V., Arcangeli, G., Fittipaldi, A., Giacca, M. and Beltram, F. (2003) Caveolae-mediated internalization of extracellular HIV-1 tat fusion proteins visualized in real time. Mol. Ther. 8, 284-294.
19. Sandgren, S., Cheng, F. and Belting, M. (2002) Nuclear targeting of macromolecular polyanions by an HIV-Tat derived peptide. Role for cell-surface proteoglycans. J. Biol. Chem. 277, 38877-38883.
20. Lin, Y. Z., Yao, S. Y., Veach, R. A., Torgerson, T. R. and Hawiger, J. (1995) Inhibition of nuclear translocation of transcription factor NF-κB by a synthetic peptide containing a cell membrane-permealbe motif and nuclear localization sequence. J. Biol. Chem. 270, 14255-14258.
21. Hawiger, J. (1999) Noninvasive intracellular delivery of functional peptides and proteins. Curr. Opin. Chem. Biol. 3, 89-94.
22. Eum, W. S., Kim D. W., Hwang, I. K., Yoo, K. Y., Kang, T. C., Jang, S. H., Choi, H. S., Choi, S. H., Kim, Y. H., Kim, S. Y., Kwon, H. Y., Kang, J. H., Kwon, O. S., Cho, S. W., Lee, K. S., Park, J., Won, M. H. and Choi, S. Y. (2004) In vivo protein transduction: biologically active intact PEP-1-superoxide dismutase fusion protein efficiently protects against ischemic insult. Free Radic. Biol. Med. 37, 1656-1669.
23. Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
24. MaCord, J. M. and Fridovich, I. (1969) Superoxide dismutase. J. Biol. Chem. 244, 6049-6055.
25. Kang, J. H. (2007) Salsolinol, a tetrahydroisoquinoline catechol neurotoxin, induces human Cu,Zn-superoxide dismutase modificaiton. BMB reports (formerly J. Biochem. Mol. Biol.) 40, 684-689.
26. Nare, B., Allocco, J. J., Kuningas, R., Galuska, S., Myers, R. W., Bednarek, M. A. and Schmatz, D. M. (1999) Development of a scintillation proximity assay for histone deacetylase using a biotinylated peptide derived from histone-H4. Anal. Biochem. 267, 390-396.
27. Park, J., Ryu, J., Kim, K. A., Lee, H. J., Bahn, J. H., Han, K., Choi, E. Y., Lee, K. S., Kwon, H. Y. and Choi, S. Y. (2002) Mutational analysis of a human immunodeficiency virus type 1 Tat protein transduction domain which is required for delivery of an exogenous protein into mammalian cells. J. Gen. Virol. 83, 1173-1181.
28. Halliwell, B. and Gutteridge, J. M. C. (1999) Free radicals in biology and medicine, Oxford University Press, Oxford.