HIV-1 Tat-mediated protein transduction of Cu,Zn-superoxide dismutase into pancreatic β cells in vitro and in vivo

Free Radical Biology and Medicine

Volumn 37, Issue 3, 2004, Pages 339-349

  Eum, Won Sik ^a   Choung, In Soon ^a   Li, Ming Zhen ^a   Kang, Jung Hoon ^b   Kim, Dae Won ^a   Park, Jinseu ^a   Kwon, Hyeok Yil ^a   Choi, Soo Young ^a  

^a Hallym University   (South Korea)

^b Chongju University   (South Korea)

Author keywords

Cu,Zn SOD; Cu,Zn superoxide dismutase; Diabetes; Free radicals; HIV 1; HIV 1 Tat; human immunodeficiency virus type 1; IDDM; insulin dependent diabetes mellitus; Oxidative stress; PBS; Tat; transactivator of transcription; Transduction

Indexed keywords

COPPER ZINC SUPEROXIDE DISMUTASE; DNA FRAGMENT; HYBRID PROTEIN; INSULIN; NITRIC OXIDE; REACTIVE OXYGEN METABOLITE; SCAVENGER; STREPTOZOCIN; TRANSACTIVATOR PROTEIN; VIRUS PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL DEATH; CONTROLLED STUDY; DIABETES MELLITUS; ENZYME ACTIVITY; HUMAN IMMUNODEFICIENCY VIRUS 1; INSULINOMA; LIPID PEROXIDATION; MALE; MOUSE; NONHUMAN; OXIDATIVE STRESS; PANCREAS; PANCREAS ISLET BETA CELL; PANCREAS ISLET CELL; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN TRANSPORT; RAT;

ANIMALS; CELL DEATH; CELLS, CULTURED; DIABETES MELLITUS, EXPERIMENTAL; GENE PRODUCTS, TAT; HIV-1; INSULIN; ISLETS OF LANGERHANS; MICE; NITRIC OXIDE; OXIDATIVE STRESS; RATS; REACTIVE OXYGEN SPECIES; RECOMBINANT FUSION PROTEINS; SUPEROXIDE DISMUTASE; TRANSDUCTION, GENETIC;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 3042679656     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2004.04.036     Document Type: Article

References (49)

1. Bach J.F. Insulin-dependent diabetes mellitus as an autoimmune disease. Endocr. Rev. 15:1994;516-542
2. Atkinson M.A., Eisenbarth G.S. Type 1 diabetes: new perspectives on disease pathogenesis and treatment. Lancet. 358:2001;221-229
3. Jaeckel E., Manns M., Von Herrath M. Viruses and diabetes. Ann. N. Y. Acad. Sci. 958:2002;7-25
4. Rabinovitch A., Suarez-Pinzon W.L., Strynadka K., Lakey J.R., Rajotte R.V. Human pancreatic islet beta-cell destruction by cytokines involves oxygen free radicals and aldehyde production. J. Clin. Endocrinol. Metab. 81:1996;3197-3202
5. Kubisch H.M., Wang J., Bray T.M., Phillips J.P. Targeted overexpression of Cu/Zn superoxide dismutase protects pancreatic beta-cells against oxidative stress. Diabetes. 46:1997;1563-1566
6. Sjoholm A. Aspects of the involvement of interleukin-1 and nitric oxide in the pathogenesis of insulin-dependent diabetes mellitus. Cell Death Differ. 5:1998;461-468
7. Lakey J.R., Suarez-Pinzon W.L., Strynadka K., Korbutt G.S., Rajotte R.V., Mabley J.G., Szabo C., Rabinovitch A. Peroxynitrite is a mediator of cytokine-induced destruction of human pancreatic islet beta cells. Lab. Invest. 81:2001;1683-1692
8. Ho E., Chen G., Bray T.M. Supplementation of N-acetylcysteine inhibits NFkappaB activation and protects against alloxan-induced diabetes in CD-1 mice. FASEB J. 13:1999;1845-1854
9. Ho E., Bray T.M. Antioxidants, NFkappaB activation, and diabetogenesis. Proc. Soc. Exp. Biol. Med. 222:1999;205-213
10. Suarez-Pinzon W.L., Szabo C., Rabinovitch A. Development of autoimmune diabetes in NOD mice is associated with the formation of peroxynitrite in pancreatic islet beta-cells. Diabetes. 46:1997;907-911
11. Hensley K., Robinson K.A., Gabbita S.P., Salsman S., Floyd R.A. Reactive oxygen species, cell signaling, and cell injury. Free Radic. Biol. Med. 28:2000;1456-1462
12. Fridovich I. Superoxide radical and superoxide dismutases. Annu. Rev. Biochem. 64:1995;97-112
13. Lenzen S., Drinkgern J., Tiedge M. Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues. Free Radic. Biol. Med. 20:1996;463-466
14. Tiedge M., Lortz S., Drinkgern J., Lenzen S. Relation between antioxidant enzyme gene expression and antioxidative defense status of insulin-producing cells. Diabetes. 46:1997;1733-1742
15. Kubisch H.M., Wang J., Luche R., Carlson E., Bray T.M., Epstein C.J., Phillips J.P. Transgenic copper/zinc superoxide dismutase modulates susceptibility to type I diabetes. Proc. Natl. Acad. Sci. USA. 91:1994;9956-9959
16. Benhamou P.Y., Moriscot C., Richard M.J., Beatrix O., Badet L., Pattou F., Kerr-Conte J., Chroboczek J., Lemarchand P., Halimi S. Adenovirus-mediated catalase gene transfer reduces oxidant stress in human, porcine and rat pancreatic islets. Diabetologia. 41:1998;1093-1100
17. Tiedge M., Lortz S., Munday R., Lenzen S. Protection against the co-operative toxicity of nitric oxide and oxygen free radicals by overexpression of antioxidant enzymes in bioengineered insulin-producing RINm5F cells. Diabetologia. 42:1999;849-855
18. Lortz S., Tiedge M., Nachtwey T., Karlsen A.E., Nerup J., Lenzen S. Protection of insulin-producing RINm5F cells against cytokine-mediated toxicity through overexpression of antioxidant enzymes. Diabetes. 49:2000;1123-1130
19. Moriscot C., Pattou F., Kerr-Conte J., Richard M.J., Lemarchand P., Benhamou P.Y. Contribution of adenoviral-mediated superoxide dismutase gene transfer to the reduction in nitric oxide-induced cytotoxicity on human islets and INS-1 insulin-secreting cells. Diabetologia. 43:2000;625-631
20. Frankel A.D., Pabo C.O. Cellular uptake of the tat protein from human immunodeficiency virus. Cell. 55:1988;1189-1193
21. Derrosi D., Joliot A.H., Chassaing G., Prochiantz A. The third helix of the Antennapedia homeodomain translocates through biological membranes. J. Biol. Chem. 269:1994;10444-10450
22. Elliott G., O'Hare P. Intercellular trafficking and protein delivery by a herpesvirus structural protein. Cell. 88:1997;223-233
23. Derrosi D., Calvet S., Trembleau A., Brunissen A., Chassaing G., Prochiantz A. Cell internalization of the third helix of the antennapedia homeodomain is receptor-independent. J. Biol. Chem. 271:1996;18188-18193
24. Vivès E., Brodin P., Lebleu B. A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J. Biol. Chem. 272:1997;16010-16017
25. Park J., Ryu J., Kim K.A., Lee H.J., Bahn J.H., Han K.H., Choi E.Y., Lee K.S., Kwon H.Y., Choi S.Y. Mutational analysis of a human immunodeficiency virus type 1 Tat protein transduction domain which is required for delivery of an exogenous protein into mammalian cells. J. Gen. Virol. 83:2002;1173-1181
26. Han K.H., Jeon M.J., Kim K.A., Park J., Choi S.Y. Efficient intracellular delivery of GFP by homeodomains of Drosophila fushi-tarazu and engrailed proteins. Mol. Cells. 10:2000;727-731
27. Han K.H., Jeon M.J., Kim S.H., Ki D., Bahn J.H., Lee K.S., Park J., Choi S.Y. Efficient intracellular delivery of an exogenous protein GFP with genetically fused basic oligopeptides. Mol. Cells. 12:2001;267-271
28. Kwon H.Y., Eum W.S., Jang H.W., Kang J.H., Ryu J., Lee B.R., Jin L.H., Park J., Choi S.Y. Transduction of Cu, Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into mammalian cells. FEBS Lett. 485:2000;163-167
29. Eum W.S., Choung I.S., Kim A.Y., Lee Y.J., Kang J.H., Park J., Lee K.S., Kwon H.Y., Choi S.Y. Transduction efficacy of Tat-Cu, Zn-superoxide dismutase is enhanced by copper ion recovery of the fusion protein. Mol. Cells. 13:2002;334-340
30. Park J., Ryu J., Jin L.H., Bahn J.H., Kim J.A., Yoon C.S., Kim D.W., Han K.H., Eum W.S., Kwon H.Y., Kang T.C., Won M.H., Kang J.H., Cho S.W., Choi S.Y. 9-Polylysine protein transduction domain: enhanced penetration efficiency of superoxide dismutase into mammalian cells and skin. Mol. Cells. 13:2002;202-208
31. Jin L.H., Bahn J.H., Eum W.S., Kwon H.Y., Jang S.H., Han K.H., Kang T.C., Won M.H., Kang J.H., Cho S.W., Park J., Choi S.Y. Transduction of human catalase mediated by an HIV-1 Tat protein basic domain and arginine-rich peptides into mammalian cells. Free Radic. Biol. Med. 31:2001;1509-1519
32. Embury J., Klein D., Pileggi A., Ribeiro M., Jayaraman S., Molano R.D., Fraker C., Kenyon N., Ricordi C., Inverardi L., Pastori R.L. Proteins linked to a protein transduction domain efficiently transduce pancreatic islets. Diabetes. 50:2001;1706-1713
33. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol. Res. 50:2001;536-546
34. Kwon N.S., Lee S.H., Choi C.H., Kho T., Lee H.S. Nitric oxide generation from streptozotocin. FASEB J. 8:1994;529-536
35. Suk K., Kim S., Kim Y.H., Kim K.A., Chang I., Yagita H., Shong M., Lee M.S. IFN-gamma/TNF-alpha synergism as the final effector in autoimmune diabetes: a key role for STAT1/IFN regulatory factor-1 pathway in pancreatic beta cell death. J. Immunol. 166:2001;4481-4489
36. McCord J.M., Fridovich I. Superoxide dismutase. J. Biol. Chem. 244:1969;6049-6055
37. Misko T.P., Schilling R.J., Salvemini D., Moore W.M., Currie M.G. A fluorometric assay for the measurement of nitrite in biological samples. Anal. Biochem. 214:1993;11-16
38. Buege J.A., Aust S.D. Microsomal lipid peroxidation. Methods Enzymol. 52:1978;302-310
39. Iwahashi H., Hanafusa T., Eguchi Y., Nakajima H., Miyagawa J., Itoh N., Tomita K., Namba M., Kuwajima M., Noguchi T., Tsujimoto Y., Matsuzawa Y. Cytokine-induced apoptotic cell death in a mouse pancreatic beta-cell line: inhibition by Bcl-2. Diabetologia. 39:1996;530-536
40. Sardas S., Yilmaz M., Oztok U., Cakir N., Karakaya A.E. Assessment of DNA strand breakage by comet assay in diabetic patients and the role of antioxidant supplementation. Mutat. Res. 490:2001;123-129
41. Mandrup-Poulsen T. Beta-cell apoptosis: stimuli and signaling. Diabetes. 50:2001;S58-S63
42. Verma I.M., Somia N. Gene therapy-promises, problems and prospects. Nature. 89:1997;239-242
43. Schwarze S.R., Ho A., Vocero-Akbani A., Dowdy S.F. In vivo protein transduction: delivery of a biologically active protein into the mouse. Science. 285:1999;1569-1572
44. Caron N.J., Torrente Y., Camirand G., Bujold M., Chapdelaine P., Leriche K., Bresolin N., Tremblay J.P. Intracellular delivery of a Tat-eGFP fusion protein into muscle cells. Mol. Ther. 3:2001;310-318
45. Gandy S.E., Buse M.G., Crouch R.K. Protective role of superoxide dismutase against diabetogenic drugs. J. Clin. Invest. 70:1982;650-658
46. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol. Res. 50:2001;537-546
47. Richard J.P., Melikov K., Vives E., Ramos C., Verbeure B., Gait M.J., Chernomordik L.V., Lebleu B. Cell-penetrating peptides: a reevaluation of the mechanism of cellular uptake. J. Biol. Chem. 278:2003;585-590
48. Cashman S.M., Morris D.J., Kumar-Singh R. Evidence of protein transduction but not intracellular transport by protein fused to HIV Tat in retinal cell culture and in vivo. Mol. Ther. 8:2003;130-142
49. Brewis N., Phelan A., Webb J., Drew J., Elliott G., O'Hare P. Evaluation of VP22 spread in tissue culture. J. Viol. 74:2000;1051-1056