A homogeneous scintillation proximity format for monitoring the activity of recombinant human long-chain-fatty-acyl-CoA synthetase 5

Assay and Drug Development Technologies

Volumn 2, Issue 3, 2004, Pages 300-307

  Bembenek, Michael E ^a   Roy, Rebecca ^a   Li, Ping ^a   Chee, Linda ^b   Jain, Sadhana ^c   Parsons, Thomas ^a  

^a MILLENNIUM PHARMACEUTICALS INC   (United States)

^b Wyeth Research ^*  (United States)

^c PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ION; LIPID; LONG CHAIN FATTY ACID COENZYME A LIGASE; LONG CHAIN FATTY ACID COENZYME A LIGASE 5; RECOMBINANT ENZYME; SILICON; UNCLASSIFIED DRUG; YTTRIUM;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DATA ANALYSIS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; HIGH THROUGHPUT SCREENING; HUMAN; LIPID METABOLISM; MEASUREMENT; MONITORING; NONHUMAN; RADIOMETRY; SCINTILLATION;

EID: 4043085004     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/1540658041410597     Document Type: Article

References (19)

1. Coleman RA, Lewin TM, Van Horn CG, Gonzalez-Baro MR: Do long-chain acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways? J Nutr 2002;132:2123-2126.
2. Prentki M, Corkey BE: Are the beta-cell signaling molecules malonyl-CoA and cystolic long-chain acyl-CoA implicated in multiple tissue defects of obesity and NIDDM? Diabetes 1996;45:273-283.
3. Faergeman NJ, Knudsen J: Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling. Biochem J 1997;323:1-12.
4. Stahl A: A current review of fatty acid transport proteins (SLC27). Pflugers Arch. 2004;447:722-727.
5. Iijima H, Fujino T, Minekura H, Suzuki H, Kang MJ, Yamamoto T: Biochemical studies of two rat acyl-CoA synthetases, ACS1 and ACS2. Eur J Biochem 1996;242: 186-190.
6. Fujino T, Kang MJ, Suzuki H, Iijima H, Yamamoto T: Molecular characterization and expression of rat acyl-CoA synthetase 3. J Biol Chem 1996;271:16748-16752.
7. Oikawa E, Iijima H, Suzuki T, Sasano H, Sato H, Kamataki A, Nagura H, Kang MJ, Fujino T, Suzuki H, Yamamoto TT: A novel acyl-CoA synthetase, ACS5, expressed in intestinal epithelial cells and proliferating preadipocytes. J Biochem (Tokyo) 1998;124:679-685.
8. Kim JH, Lewin TM, Coleman RA: Expression and characterization of recombinant rat acyl-CoA synthetases 1, 4, and 5. Selective inhibition by triacsin C and thiazolidinediones. J Biol Chem 2001;276:24667-24673.
9. Malhotra KT, Malhotra K, Lubin BH, Kuypers FA: Identification and molecular characterization of acyl-CoA synthetase in human erythrocytes and erythroid precursors. Biochem J 1999;344:135-143.
10. Yamashita Y, Kumabe T, Cho YY, Watanabe M, Kawagishi J, Yoshimoto T, Fujino T, Kang MJ, Yamamoto TT: Fatty acid induced glioma cell growth is mediated by the acyl-CoA synthetase 5 gene located on chromosome 10q25.1-q25.2, a region frequently deleted in malignant gliomas. Oncogene 2000;19:5919-5925.
11. Gassler N, Schneider A, Kopitz J, Schnolzer M, Obermuller N, Kartenbeck J, Otto HF, Autschbach F: Impaired expression of acyl-CoA-synthetase 5 in epithelial tumors of the small intestine. Hum Pathol 2003;34: 1048-1052.
12. Vanhoof G, Berry J, Harvey M, Price-Jones M, Hughes K: A high throughput screening assay for thiamin pyrophosphate kinase activity using SPA. Available at: http://www1.amershambiosciences.com/aptrix/upp00919.nsf/Content/ DrugScr%20SPA%201ntroduction%5CDrugScr%20SPA%20Publications%5CSPA%20 Posters?OpenDocument&hometitle=DrugScr. Last accessed Spring, 2004.
13. Brandish PE, Hill LA, Zheng W, Scolnick EM: Scintillation proximity assay of inositol phosphates in cell extracts: high-throughput measurement of G-protein-coupled receptor activation. Anal Biochem 2003;313:311-318.
14. Macarron R, Mensah L, Cid C, Carranza C, Benson N, Pope AJ, Diez E: A homogeneous method to measure aminoacyl-tRNA synthetase aminoacylation activity using scintillation proximity assay technology. Anal Biochem 2000;284:183-190.
15. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72: 248-254.
16. Juguelin H, Cassagne C: Assay of long-chain acyl-CoAs in a complex reaction mixture. Anal Biochem 1984; 142:329-335.
17. Spector AA, John K, Fletcher JE: Binding of long-chain fatty acids to bovine serum albumin. J Lipid Res 1969; 10:56-67.
18. Mayorek N, Bar-Tana J: Inhibition of diacylglycerol acyltransferase by 2-bromooctanoate in cultured rat hepatocytes. J Biol Chem 1985;260:6528-6532.
19. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999; 4:67-73.