Scintillation proximity assay of inositol phosphates in cell extracts: High-throughput measurement of G-protein-coupled receptor activation

Analytical Biochemistry

Volumn 313, Issue 2, 2003, Pages 311-318

  Brandish, Philip E ^a   Hill, Lorraine A ^a   Zheng, Wei ^a   Scolnick, Edward M ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

GPCR assay; High throughput screening; Inositol phosphate; PI turnover assay; Scintillation proximity assay

Indexed keywords

ALCOHOLS; CHEMICAL ACTIVATION; CHROMATOGRAPHY; LITHIUM COMPOUNDS; PHOSPHATES; RECOMBINANT PROTEINS; SCINTILLATION; SILICATES; SUGARS; YTTRIUM COMPOUNDS;

ANION EXCHANGE CHROMATOGRAPHY; G PROTEIN COUPLED RECEPTORS; HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT MEASUREMENTS; INOSITOL PHOSPHATE; MUSCARINIC ACETYLCHOLINE RECEPTORS; PHOSPHATIDYLINOSITOL; PHOSPHATIDYLINOSITOL 4 ,5-BISPHOSPHATE;

POLYOLS;

ATROPINE; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; INOSITOL; INOSITOL PHOSPHATE; MUSCARINIC RECEPTOR; MUSCARINIC RECEPTOR BLOCKING AGENT; NEUROPEPTIDE RECEPTOR; PIRENZEPINE; TRITIUM; YTTRIUM;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; AUTOMATION; CELL LABELING; CONTROLLED STUDY; DEPHOSPHORYLATION; DRUG RECEPTOR BINDING; HUMAN; HUMAN CELL; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN METABOLISM; RECEPTOR UPREGULATION; SCINTILLATION; SEPARATION TECHNIQUE; VALIDATION PROCESS;

EID: 0037442467     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00630-9     Document Type: Article

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